Yıl 2018, Cilt 4, Sayı 3, Sayfalar 1083 - 1093 2018-12-15

The effects of transfer day in intracytoplasmic sperm injection (ICSI) pregnancies on first trimester screening test results
The effects of transfer day in intracytoplasmic sperm injection (ICSI) pregnancies on first trimester screening test results

Nur Dokuzeylül Güngör [1] , Tuğba Gürbüz [2] , Aynur Erşahin [3]

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Objects: To compare first trimesterscrening (FTS) serum markers free β-human chorionic gonodotropin (free β-hCG and pregnancy associated plasma protein A (PAPP-A)  of blastocyst versus cleavage stage embryo transfers after assisted reproductive technologies (ART).

Methods: Retrospective examinations of 123 women who conceived with ART were subjected to FTS from January 2017 to December 2017 at Bahçeşehir University Göztepe Medical Park Hospital In Vitro Fertilization (IVF) Unit.The age,gestational week,ultrasound (US) markers and biochemistry markers(PAPP-A,free β-hCG) were collected.Outcomes were regarded as meaningful when the p < 0.05. Statistical Package For Social Sciences (SPSS) v.17.0 for Windows was preferred to make statistical analysis.

Results: PAPP-A amounts were remarkably low in day-3 (cleavage stage embryos) transfer intracytoplasmic sperm injection (ICSI) pregnancies compared to day-5 (blastocyst stage embryos) transfer ICSI pregnancies.Free β-hCG amounts weren’t significantly different in two groups (p=0.371). NT was unaffected by the transferenceday (p=0.21). Also CRL wasunaffected by the transferenceday (p=0.693). No differences appeared between 3rd and 5th day embryo transference according to maternal age (p=0.616), weight (p=0.693) and gestational age (p=0.742) at sampling.

Conclusion: Today, the datas about the effects of ART over the ingredients of combined FTS for chromosomal analysis are contentious yet.Day-3 ICSI pregnancies had meaningfully low PAPP-A amounts which support the requirement to properly set the combined FTS risks algoritm. These outcomes are likely to be because of changings in the placenta of ART pregnancies.

Objects

To compare first trimester screning serum markers free β-human chorionic gonodotropin (free β-hCG and pregnancy associated plasma protein A (PAPP-A)  of blastocyst versus cleavage stage embryo transfers after assisted reproductive technologies (ART) .

Methods

Retrospective analysis of 123 women who conceived with ART undergone first-trimester  screening between January 2017 and December 2017 at Bahçeşehir University Göztepe Medical Park Hospital  IVF Unit. The age, gestational week, ultrasound markers and biochemistry  markers (PAPP- A,free β-hCG) were collected. Results were considered significant when the p value was less than 0.05. Statistical Package For Social Sciences (SPSS) version 17.0 for Windows was used for statistical analysis.

Results

PAPP-A levels were significantly lower in day-3 (cleavage stage embryos) transfer intracytoplasmic sperm injection (ICSI) pregnancies compared to day-5 (blastocyst stage embryos) transfer ICSI pregnancies (p<0.05). Free β-hCG levels weren’t significantly differ in two groups (p=0.371). NT was unaffected by the day of transfer (p=0.21). Also CRL wasn’t affected by the day of transfer (p=0.693). No differences appeared between day-3 and day-5 embryo transfer with respect to maternal age ,(p=0.616) weight (p=0.693) and gestational age (p=0.742)  at sampling.

 

Conclusion

Today, the data on the effect of ART on the components of first trimester combined screening

for chromosomal analysis are still controversial. Day-3  ICSI  pregnancies  had significantly

lower PAPP-A levels supporting the need to appropriately adjust the combined first trimester

screening (cFTS) risk algoritm. These results may be due to alterations in the placentation of ART pregnancies.

  • 1.Mangalraj AM, Muthukumar K, Aleyamma T, Kamath MS, George K. Blastocyst stage transfer vs cleavage stage embryo transfer. J Hum Reprod Sci 2009;2:23–6.
  • 2.Karaki RZ, Samarraie SS, Younis NA, Lahloub TM, Ibrahim MH. Blastocyst culture andtransfer: a step toward improved in vitro fertilization outcome. Fertil Steril 2002;77:114-18.
  • 3.Edwards RG, Beard HK. Blastocyst stage transfer: pitfalls and benefits. Hum Reprod. 1999;14:1–4.
  • 4.Gardner DK, Lane M. Culture and selection of viable blastocysts: a feasible proposition for human IVF? Hum Reprod Update. 1997;3:367–82.
  • 5.Pool TB, Atiee SR, Martin JE. Oocyte and embryo culture: basic concepts and recent advances. Infert Reprod Med Clinics N Amer. 1998;9:181–203.
  • 6.Tsirigotis M. Blastocyst stage transfer: pitfalls and benefits. Too soon to abandon current practice? Hum Reprod. 1998;13:3285–89.
  • 7.Gardner DK, Schoolcraft WB. No longer neglected: the human blastocyst. Hum Reprod. 1998;13:3289–92.
  • 8.Gardner DK, Balaban B. Choosing between day 3 and day 5 embryo transfers. Clin Obstet Gynecol. 2006;49:85–92.
  • 9.Nicolaides KH. Screening for chromosomal defects. Ultrasound Obstet Gynecol. 2003;21:313–21.
  • 10.Royal Australian and New Zealand College of Obstetricians and Gynaecologists and Human Genetics Society of Australasia. Prenatal screening tests for trisomy 21 (Down syndrome), trisomy18 (Edwards syndrome) and neural tube defects. Victoria: RoyalAustralian and New Zealand College of Obstetricians andGynaecologists; 2007.
  • 11.Nicolaides KH. A model for a new pyramid of prenatal care based on the 11 to 13 weeks' assessment. Prenat Diagn 2011; 31: 3-6.
  • 12.Sonek J, Nicolaides KH. Additional first-trimester ultrasound markers. Clin Lab Med 2010; 30: 573-92.
  • 13.Kagan KO, Wright D, Spencer K et al. First-trimester screening for trisomy 21 by free beta-human chorionic gonadotropin and pregnancy-associated plasma protein-A: impact of maternal and pregnancy characteristics. Ultrasound Obstet Gynecol 2008; 31: 493-502.
  • 14.Chitayat DS, Langlois S, Wilson RD. Prenatal screening for fetal aneuploidy in singleton pregnancies. J Obstet Gynaecol Can 2011;33:736-50.
  • 15.Spencer K, Staboulidou I, Nicolaides KH. First trimester aneuploidy screening in the presence of a vanishing twin: implications for maternal serum markers. Prenat Diagn 2010; 30: 235-40.
  • 16.Klemetti R, Gissler M, Hemminki E. Comparison of perinatal health of children born from IVF in Finland in the early and late 1990s. Hum Reprod. 2002;17(8):2192–8.
  • 17.Wright VC, Chang J, Jeng G, Macaluso M. Centers for disease C, prevention. Assisted reproductive technology surveillance—United States, 2005. MMWR Surveill Summ. 2008;57(5):1–23.
  • 18.Chen H, Egan JO, Ohlu JF. Regulation and activities of alpha-fetoprotein, Crit Rev Eukaryot Gene Expr 1997; 7: 11-41.
  • 19.Frendo JL, Vidaud M, Guibourdenche J, Luton D, Muller F, Bellet D, et al. Defect of villous cytotrophoblast differentiation into syncytiotrophoblast in Down's syndrome, J Clin Endocrinol Metab 2000; 85: 3700-07.
  • 20.Krantz DA, Larsen JW, Buchanan PD, Macri JN. First-trimester Down syndrome screening: free beta-human chorionic gonadotropin and pregnancy-associated plasma protein A, Am J Obstet Gynecol 1996;174: 612-16.
  • 21.Spencer K. Evaluation of an assay of the free beta-subunit of choriogonadotropin andits potential value in screening for Down's syndrome. Clin Chem 1991;37:809-14.
  • 22.Almog B, Al-Shalaty J, Sheizaf B, Shehata F, Son WY, Tan SL, et al. Difference between serum beta-human chorionic gonadotropin levels in pregnancies after in vitro maturation and in vitro fertilization treatments. Fertil Steril 2011; 95: 85-8.
  • 23.Cole LA. Immunoassay of human gonadotropin, its free subunits, and metabolites, Clin Chem 1997;43:2233-43.
  • 24.Spencer K. Second-trimester prenatal screening for Down syndrome and the relationship of maternal serum biochemical markers to pregnancy complications with adverse outcome, Prenat Diagn 2000;20:652-56.
  • 25.Dugoff L, Hobbins JC, Malone FD, Vidaver J, Sullivan L, Canick JA, et al. Quad screen as a predictor of adverse pregnancy outcome. Obstet Gynecol 2005;106:260-67.
  • 26.Spencer K, Crossley JA, Aitken DA, Nix AB, Dunstan FD, Williams K. The effect of temporal variation in biochemical markers of trisomy 21 across the first and second trimesters of pregnancy on the estimation of individual patient-specific risks and detection rates for Down's syndrome. Ann Clin Biochem 2003;40:219-31.
  • 27.Zegers-Hochschild F, Altieri E, Fabres C, Fernandez E, Mackenna A, Orihuela P. Predictive value of human chorionic gonadotrophin in the outcome of early pregnancy after in-vitro fertilization and spontaneous conception. Hum Reprod 1994; 9:1550-55.
  • 28.Barkai G, Goldman B, Ries L, Chaki R, Dor J, Cuckle H. Down's syndrome screening marker levels following assisted reproduction Prenat Diagn 1996;16: 1111-14.
  • 29.Wald NJ, White N, Morris JK, Huttly WJ, Canick JA. Serum markers for Down's syndrome in women who have had in vitro fertilisation: implications for antenatal screening. Br J Obstet Gynaecol 1999;106:1304-06.
  • 30.Bar-Hava I, Yitzhak M, Krissi H, Shohat M, Shalev J, Czitron B, et al. Triple-test screening in in vitro fertilization pregnancies. J Assist Reprod Genet 2001;18:226-9.
  • 31.Giudice LC, Conover CA, Bale L, Faessen GH, Ilg K, Sun I, et al. Identification and regulation of the IGFBP-4 protease and its physiological inhibitor in human trophoblasts and endometrial stroma: evidence for paracrine regulation of IGF-II bioavailability in the placental bed during human implantation. J Clin Endocrinol Metab 2002;87:2359-66.
  • 32.Santolaya-Forgas J, De Leon JA, Cullen HR, Castracane VD, Kauffman RP, Sifuentes GA. Low pregnancy-associated plasma protein-a at 10 (+1) to 14(+6) weeks of gestation and a possible mechanism leading to miscarriage. Fetal Diagn Ther 2004;19: 456-61.
  • 33.Shih W, Rushford DD, Bourne H, Garrett C, McBain JC, Healy DL, et al. Factors affecting low birthweight after assisted reproduction technology: difference between transfer of fresh and cryopreserved embryos suggests an adverse effect of oocyte collection. Hum Reprod 2008;23: 1644-53.
  • 34.Pinborg A, Loft A, Aaris Henningsen AK, Rasmussen S, Andersen AN. Infant outcome of 957 singletons born after frozen embryo replacement: the Danish National Cohort Study 1995–2006. Fertil Steril 2010;94:1320-27.
  • 35.Dumoulin JC, Land JA, Van Montfoort AP, Nelissen EC, Coonen E, Derhaag JG, et al. Effect of in vitro culture of human embryos on birthweight of newborns. Hum Reprod 2010;25:605-12.
  • 36.Ranta JK, Raatikainen K, Romppanen J, Pulkki K, Heinonen S. Increased time-to-pregnancy and first trimester Down's syndrome screening. Hum Reprod 2010;25:412–7.
  • 37.Amor DJ, Xu JX, Halliday JL, Francis I, Healy DL, Breheny S, et al. Pregnancies conceived using assisted reproductive technologies (ART) have low levels of pregnancy-associated plasma protein-A (PAPP-A) leading to a high rate of false-positive results in first trimester screening for Down syndrome. Hum Reprod 2009;24:1330–8.
  • 38.Anderson UD, Olsson MG, Kristensen KH, Akerstr€om B, Hansson SR.Review: biochemical markers to predict preeclampsia. Placenta 2012;33:42–7.
  • 39.Talaulikar VS, Arulkumaran S. Reproductive outcomes after assisted conception.Obstet Gynecol Sury 2012;67:566–83.
  • 40.Bender F, Hecken J, Reinsberg J, Berg C, Van der Ven H, Gembruch U, et al. Altered first-trimester screening markers after IVF/ICSI: no relationship withsmall-for-gestational-age and number of embryos transferred. Reprod BiomedOnline 2010;20:516–22.
Birincil Dil en
Konular Sağlık Bilimleri ve Hizmetleri
Dergi Bölümü Araştırma/Research
Yazarlar

Yazar: Nur Dokuzeylül Güngör (Sorumlu Yazar)
Kurum: Göztepe Medikal Park Hastanesi
Ülke: Turkey


Yazar: Tuğba Gürbüz
Kurum: Göztepe Medikal park Hastanesi
Ülke: Turkey


Yazar: Aynur Erşahin
Kurum: Göztepe medikal park hastanesi
Ülke: Turkey


Bibtex @araştırma makalesi { adiyamansaglik440359, journal = {Adıyaman Üniversitesi Sağlık Bilimleri Dergisi}, issn = {}, eissn = {2458-9179}, address = {Adıyaman Üniversitesi}, year = {2018}, volume = {4}, pages = {1083 - 1093}, doi = {10.30569/adiyamansaglik.440359}, title = {The effects of transfer day in intracytoplasmic sperm injection (ICSI) pregnancies on first trimester screening test results}, key = {cite}, author = {Dokuzeylül Güngör, Nur and Gürbüz, Tuğba and Erşahin, Aynur} }
APA Dokuzeylül Güngör, N , Gürbüz, T , Erşahin, A . (2018). The effects of transfer day in intracytoplasmic sperm injection (ICSI) pregnancies on first trimester screening test results. Adıyaman Üniversitesi Sağlık Bilimleri Dergisi, 4 (3), 1083-1093. DOI: 10.30569/adiyamansaglik.440359
MLA Dokuzeylül Güngör, N , Gürbüz, T , Erşahin, A . "The effects of transfer day in intracytoplasmic sperm injection (ICSI) pregnancies on first trimester screening test results". Adıyaman Üniversitesi Sağlık Bilimleri Dergisi 4 (2018): 1083-1093 <http://dergipark.gov.tr/adiyamansaglik/issue/40184/440359>
Chicago Dokuzeylül Güngör, N , Gürbüz, T , Erşahin, A . "The effects of transfer day in intracytoplasmic sperm injection (ICSI) pregnancies on first trimester screening test results". Adıyaman Üniversitesi Sağlık Bilimleri Dergisi 4 (2018): 1083-1093
RIS TY - JOUR T1 - The effects of transfer day in intracytoplasmic sperm injection (ICSI) pregnancies on first trimester screening test results AU - Nur Dokuzeylül Güngör , Tuğba Gürbüz , Aynur Erşahin Y1 - 2018 PY - 2018 N1 - doi: 10.30569/adiyamansaglik.440359 DO - 10.30569/adiyamansaglik.440359 T2 - Adıyaman Üniversitesi Sağlık Bilimleri Dergisi JF - Journal JO - JOR SP - 1083 EP - 1093 VL - 4 IS - 3 SN - -2458-9179 M3 - doi: 10.30569/adiyamansaglik.440359 UR - http://dx.doi.org/10.30569/adiyamansaglik.440359 Y2 - 2018 ER -
EndNote %0 Adıyaman Üniversitesi Sağlık Bilimleri Dergisi The effects of transfer day in intracytoplasmic sperm injection (ICSI) pregnancies on first trimester screening test results %A Nur Dokuzeylül Güngör , Tuğba Gürbüz , Aynur Erşahin %T The effects of transfer day in intracytoplasmic sperm injection (ICSI) pregnancies on first trimester screening test results %D 2018 %J Adıyaman Üniversitesi Sağlık Bilimleri Dergisi %P -2458-9179 %V 4 %N 3 %R doi: 10.30569/adiyamansaglik.440359 %U 10.30569/adiyamansaglik.440359
ISNAD Dokuzeylül Güngör, Nur , Gürbüz, Tuğba , Erşahin, Aynur . "The effects of transfer day in intracytoplasmic sperm injection (ICSI) pregnancies on first trimester screening test results". Adıyaman Üniversitesi Sağlık Bilimleri Dergisi 4 / 3 (Aralık 2018): 1083-1093. http://dx.doi.org/10.30569/adiyamansaglik.440359