Yıl 2019, Cilt 3, Sayı 1, Sayfalar 1 - 6 2019-05-01

Sucul bir bitki olan Pogostemon erectus (Dalzell) Kuntze’un In Vitro hızlı çoğaltımı
In Vitro rapid propagation of an aquatic plant Pogostemon erectus (Dalzell) Kuntze

Muhammet DOĞAN [1]

19 82

Bu çalışmanın amacı, Pogostemon erectus (Dalzell) Kuntze'un doku kültürü teknikleri ile çoklu ve hızlı üretimini araştırmaktır. P. erectus'un sürgün ucu ve nodal eksplantları izole edilmiş ve daha sonra farklı kombinasyonlarda thidiazuron (TDZ) ve 2,4-Diklorofenoksiasetik asit (2,4-D) içeren Murashige ve Skoog (1962) (MS) besin ortamında kültüre alınmıştır. Eksplantlardaki ilk sürgün oluşumu 15. günde gözlenmiştir. Eksplant başına sürgün sayısı, sürgün ucu eksplantlarında 2.85 ila 29.39 ve nodal eksplantlarında 2.72 ila 32.24 arasında sıralanmıştır. Eksplant başına maksimum sürgün sayısı sürgün ucu eksplantı için (29.39) 0.20 mg/L TDZ + 0.10 mg/L 2,4-D içeren MS ortamında ve nodal eksplant için 0.10 mg/L TDZ + 0.10 mg/L 2,4-D içeren MS ortamında (32.24) elde edilmiştir. En uzun sürgünler, sürgün ucu (1.76 cm) ve nodal (1.64 cm) eksplantlarında 0.10 mg/L TDZ + 0.10 mg/L 2,4-D ile desteklenmiş MS ortamında belirlenmiştir. Rejenere sürgünler, 0.25 mg/L Indol-3-asetik asit (IAA) ile takviye edilmiş MS ortamında köklendirilmiş ve ardından akvaryum koşullarına alıştırılmıştır.

The aim of this study is to investigate the multiple and rapid production of Pogostemon erectus (Dalzell) Kuntze by tissue culture techniques. The shoot tip and nodal explants of P. erectus were isolated and then cultured in Murashige and Skoog (1962) (MS) nutrient media containing thidiazuron (TDZ) and 2,4-Dichlorophenoxyacetic acid (2,4-D) in different combinations. The first shoot formation on the explants was observed on day 15. The number of shoots per explant ranged from 2.85 to 29.39 in the shoot tip explants and from 2.72 to 32.24 in the nodal explants. The maximum number of shoots per explant was obtained in the MS medium containing 0.20 mg/L TDZ + 0.10 mg/L 2,4-D for shoot tip explant (29.39) and in the MS medium containing 0.10 mg/L TDZ + 0.10 mg/L 2,4-D for nodal explant (32.24). The longest shoots in the shoot tip (1.76 cm) and nodal (1.64 cm) explants were determined in MS medium supplemented with 0.10 mg/L TDZ + 0.10 mg/L 2,4-D. Regenerated shoots were rooted in MS medium supplemented with 0.25 mg/L indol-3yl acetic acid (IAA), and then acclimatized to aquarium conditions.
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Birincil Dil en
Konular Biyoloji
Yayımlanma Tarihi 2019
Dergi Bölümü Articles
Yazarlar

Orcid: 0000-0003-3138-5903
Yazar: Muhammet DOĞAN (Sorumlu Yazar)
Kurum: KARAMANOĞLU MEHMETBEY ÜNİVERSİTESİ, KAMİL ÖZDAĞ FEN FAKÜLTESİ
Ülke: Turkey


Bibtex @araştırma makalesi { ajb441197, journal = {Anatolian Journal of Botany}, issn = {}, eissn = {2602-2818}, address = {Abdullah KAYA}, year = {2019}, volume = {3}, pages = {1 - 6}, doi = {10.30616/ajb.441197}, title = {In Vitro rapid propagation of an aquatic plant Pogostemon erectus (Dalzell) Kuntze}, key = {cite}, author = {DOĞAN, Muhammet} }
APA DOĞAN, M . (2019). In Vitro rapid propagation of an aquatic plant Pogostemon erectus (Dalzell) Kuntze. Anatolian Journal of Botany, 3 (1), 1-6. DOI: 10.30616/ajb.441197
MLA DOĞAN, M . "In Vitro rapid propagation of an aquatic plant Pogostemon erectus (Dalzell) Kuntze". Anatolian Journal of Botany 3 (2019): 1-6 <http://dergipark.gov.tr/ajb/issue/40746/441197>
Chicago DOĞAN, M . "In Vitro rapid propagation of an aquatic plant Pogostemon erectus (Dalzell) Kuntze". Anatolian Journal of Botany 3 (2019): 1-6
RIS TY - JOUR T1 - In Vitro rapid propagation of an aquatic plant Pogostemon erectus (Dalzell) Kuntze AU - Muhammet DOĞAN Y1 - 2019 PY - 2019 N1 - doi: 10.30616/ajb.441197 DO - 10.30616/ajb.441197 T2 - Anatolian Journal of Botany JF - Journal JO - JOR SP - 1 EP - 6 VL - 3 IS - 1 SN - -2602-2818 M3 - doi: 10.30616/ajb.441197 UR - http://dx.doi.org/10.30616/ajb.441197 Y2 - 2018 ER -
EndNote %0 Anatolian Journal of Botany In Vitro rapid propagation of an aquatic plant Pogostemon erectus (Dalzell) Kuntze %A Muhammet DOĞAN %T In Vitro rapid propagation of an aquatic plant Pogostemon erectus (Dalzell) Kuntze %D 2019 %J Anatolian Journal of Botany %P -2602-2818 %V 3 %N 1 %R doi: 10.30616/ajb.441197 %U 10.30616/ajb.441197
ISNAD DOĞAN, Muhammet . "Sucul bir bitki olan Pogostemon erectus (Dalzell) Kuntze’un In Vitro hızlı çoğaltımı". Anatolian Journal of Botany 3 / 1 (Mayıs 2019): 1-6. http://dx.doi.org/10.30616/ajb.441197