Yıl 2017, Cilt 15, Sayı 2, Sayfalar 109 - 118 2017-08-11

Narın Farklı Bölümlerinin Polifenol İçeriği, Antioksidan ve Antibakteriyel Aktivitesi Üzerine Ekstraksiyon Çözgenlerinin Etkisi
Effects of Extraction Solvents on Polyphenol Contents, Antioxidant and Antibacterial Activities of Pomegranate Parts

Meltem Türkyılmaz [1] , Şeref Tağı [2] , Mehmet Özkan [3]

326 298

Nar kabuğu, dilim zarı ve çekirdeklerinde bulunan polifenoller; aseton, aseton:su (70:30, v:v), metanol, etanol ve su ile 4°C’de 24 saat süresince ekstrakte edilmiştir. Nar kabuğu ve dilim zarında; gallik asit, punikalin, a-punikalajin, b-punikalajin ve ellajik asit türevleri HPLC ile tanımlanırken, çekirdekte sadece elajik asit türevleri tanımlanmıştır. Nar kabuğu ve dilim zarının çok yüksek miktarda polifenol içerdiği belirlenmiştir. Nar kabuğu (r=0.967), dilim zarı (r=0.976) ve çekirdeklerden (r=0.972) elde edilen ekstraktların toplam polifenol miktarı ve antioksidan aktiviteleri arasında güçlü korelasyonlar bulunmuştur. Ayrıca, en yüksek toplam polifenol madde miktarı ve antioksidan aktivite aseton:su (70:30, v:v) çözeltisiyle elde edilmiştir. Nar kabuğu ve dilim zarının yüksek antioksidan ve antibakteriyel aktivitesi punikalajinlerden kaynaklanmaktadır. Nar kabuğu ve dilim zarı Bacillus megaterium and Staphylococcus aureus’a karşı antibakteriyel aktivite göstermiştir. Yüksek antioksidan ve antibakteriyel aktiviteleri nedeniyle, nar kabuğu ve dilim zarının sulu ekstraktları gıda katkısı ve koruyucusu olarak kullanılabilir.

Polyphenols in rinds, membranes and seeds of pomegranates were extracted with acetone, acetone:water (70:30, v:v), methanol, ethanol and water for 12 h at 4°C. Gallic acid, punicalin, a-punicalagin, b-punicalagin and ellagic acid derivatives (EAD) were identified in rind and membrane, while only EAD were identified in seed by HPLC. The rind and membrane contained exceptionally high amounts of polyphenols. Strong correlations were found between antioxidant activity (AOA) and total polyphenol content (TPC) for the extracts of rind (r=0.967), membrane (r=0.976) and seed (r=0.972). Moreover, the highest TPC and AOA were determined in acetone:water (70:30, v:v) extracts. Punicalagins at high amounts in the pomegranate rind and membrane provided the highest AOA and antibacterial activity (ABA). The extracts of rind and membrane showed ABA against Bacillus megaterium and Staphylococcus aureus. Water extracts of rind and membrane may be used as a food additive and preservative due to their high AOA and ABA.

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Konular
Dergi Bölümü Araştırma Makaleleri
Yazarlar

Orcid: http://orcid.org/0000-0002-9505-5076
Yazar: Meltem Türkyılmaz

Orcid: http://orcid.org/0000-0003-0556-9952
Yazar: Şeref Tağı

Orcid: http://orcid.org/0000-0003-3008-6100
Yazar: Mehmet Özkan

Bibtex @araştırma makalesi { akademik-gida333632, journal = {Akademik Gıda}, issn = {1304-7582}, eissn = {2148-015X}, address = {Sidas Medya Ajans Tanıtım Danışmanlık Ltd. Şti.}, year = {2017}, volume = {15}, pages = {109 - 118}, doi = {10.24323/akademik-gida.333632}, title = {Narın Farklı Bölümlerinin Polifenol İçeriği, Antioksidan ve Antibakteriyel Aktivitesi Üzerine Ekstraksiyon Çözgenlerinin Etkisi}, key = {cite}, author = {Tağı, Şeref and Türkyılmaz, Meltem and Özkan, Mehmet} }
APA Türkyılmaz, M , Tağı, Ş , Özkan, M . (2017). Narın Farklı Bölümlerinin Polifenol İçeriği, Antioksidan ve Antibakteriyel Aktivitesi Üzerine Ekstraksiyon Çözgenlerinin Etkisi. Akademik Gıda, 15 (2), 109-118. DOI: 10.24323/akademik-gida.333632
MLA Türkyılmaz, M , Tağı, Ş , Özkan, M . "Narın Farklı Bölümlerinin Polifenol İçeriği, Antioksidan ve Antibakteriyel Aktivitesi Üzerine Ekstraksiyon Çözgenlerinin Etkisi". Akademik Gıda 15 (2017): 109-118 <http://dergipark.gov.tr/akademik-gida/issue/30836/333632>
Chicago Türkyılmaz, M , Tağı, Ş , Özkan, M . "Narın Farklı Bölümlerinin Polifenol İçeriği, Antioksidan ve Antibakteriyel Aktivitesi Üzerine Ekstraksiyon Çözgenlerinin Etkisi". Akademik Gıda 15 (2017): 109-118
RIS TY - JOUR T1 - Narın Farklı Bölümlerinin Polifenol İçeriği, Antioksidan ve Antibakteriyel Aktivitesi Üzerine Ekstraksiyon Çözgenlerinin Etkisi AU - Meltem Türkyılmaz , Şeref Tağı , Mehmet Özkan Y1 - 2017 PY - 2017 N1 - doi: 10.24323/akademik-gida.333632 DO - 10.24323/akademik-gida.333632 T2 - Akademik Gıda JF - Journal JO - JOR SP - 109 EP - 118 VL - 15 IS - 2 SN - 1304-7582-2148-015X M3 - doi: 10.24323/akademik-gida.333632 UR - http://dx.doi.org/10.24323/akademik-gida.333632 Y2 - 2017 ER -
EndNote %0 Akademik Gıda Narın Farklı Bölümlerinin Polifenol İçeriği, Antioksidan ve Antibakteriyel Aktivitesi Üzerine Ekstraksiyon Çözgenlerinin Etkisi %A Meltem Türkyılmaz , Şeref Tağı , Mehmet Özkan %T Narın Farklı Bölümlerinin Polifenol İçeriği, Antioksidan ve Antibakteriyel Aktivitesi Üzerine Ekstraksiyon Çözgenlerinin Etkisi %D 2017 %J Akademik Gıda %P 1304-7582-2148-015X %V 15 %N 2 %R doi: 10.24323/akademik-gida.333632 %U 10.24323/akademik-gida.333632
ISNAD Türkyılmaz, Meltem , Tağı, Şeref , Özkan, Mehmet . "Narın Farklı Bölümlerinin Polifenol İçeriği, Antioksidan ve Antibakteriyel Aktivitesi Üzerine Ekstraksiyon Çözgenlerinin Etkisi". Akademik Gıda 15 / 2 (Ağustos 2017): 109-118. http://dx.doi.org/10.24323/akademik-gida.333632