Yıl 2017, Cilt 11, Sayı 2, Sayfalar 72 - 78 2017-08-20

Chronic anaemia causes degenerative changes in trophoblast cells of the rat placenta

Omer Awad [1] , Shem J. Ochieng [2] , Abdel Malek [3] , Julius Ogeng’o [4]

65 258

Objectives: Iron deficiency anaemia causes adverse pregnancy outcome. There are few studies on effects of anaemia on the

structure of trophoblastic cells which are important in placental function. These data are important for understanding the

function and disorders of the placenta. The aim of this study was to investigate the ultrastructural cellular changes associated

with iron deficiency anaemia in rat placenta.

Methods: Forty-nine female Sprague-Dawley rats were randomly separated into experimental and control groups. The

experimental group was rendered anaemic by removing 1.5 ml of blood per bleed on five alternate days, and the placentas

were collected on gestational days 17, 19 and 21. For light microscopy, five cubic millimeter segments were fixed in 10%

buffered formaldehyde solution; dehydrated in ethanol and embedded in paraffin wax. Five micron thick sections were cut,

deparaffinized and stained with Hematoxylin and Eosin. For transmission electron microscopy, 1 mm3 sections were fixed in

2.5% phosphate buffered glutaraldehyde, post fixed in 2% osmium tetroxide, dehydrated in ethanol, cleared in propylene

and embedded in epon resin. Ultrathin sections stained with uranyl acetate and lead citrate were examined with JEOL electron


Results: Cytotrophoblast, syncytiotrophoblast and giant trophoblastic cells of placentas of anaemic rats showed cytoplasmic

and nuclear vacuolation with loss of cell margins. In addition, there was atrophy of microvilli on the cell surface, as well

nuclear chromatolysis, nucleolar degeneration and appearance of dark bodies.

Conclusion: Chronic anaemia causes trophoblastic cell degeneration. This may undermine the functional integrity of the

cells and constitute part of the mechanism for poor fetal outcome.

anaemia; cytotrophoblast; degeneration; giant cells; placenta; syncytiotrophoblast
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Dergi Bölümü Original Articles

Yazar: Omer Awad
Ülke: Kenya

Yazar: Shem J. Ochieng
Ülke: Kenya

Yazar: Abdel Malek
Ülke: Kenya

Yazar: Julius Ogeng’o
Ülke: Kenya

Bibtex @araştırma makalesi { anatomy341920, journal = {Anatomy}, issn = {1307-8798}, eissn = {1308-8459}, address = {Türk Anatomi ve Klinik Anatomi Derneği}, year = {2017}, volume = {11}, pages = {72 - 78}, doi = {}, title = {Chronic anaemia causes degenerative changes in trophoblast cells of the rat placenta}, key = {cite}, author = {Ochieng, Shem J. and Awad, Omer and Ogeng’o, Julius and Malek, Abdel} }
APA Awad, O , Ochieng, S , Malek, A , Ogeng’o, J . (2017). Chronic anaemia causes degenerative changes in trophoblast cells of the rat placenta. Anatomy, 11 (2), 72-78. Retrieved from http://dergipark.gov.tr/anatomy/issue/31323/341920
MLA Awad, O , Ochieng, S , Malek, A , Ogeng’o, J . "Chronic anaemia causes degenerative changes in trophoblast cells of the rat placenta". Anatomy 11 (2017): 72-78 <http://dergipark.gov.tr/anatomy/issue/31323/341920>
Chicago Awad, O , Ochieng, S , Malek, A , Ogeng’o, J . "Chronic anaemia causes degenerative changes in trophoblast cells of the rat placenta". Anatomy 11 (2017): 72-78
RIS TY - JOUR T1 - Chronic anaemia causes degenerative changes in trophoblast cells of the rat placenta AU - Omer Awad , Shem J. Ochieng , Abdel Malek , Julius Ogeng’o Y1 - 2017 PY - 2017 N1 - DO - T2 - Anatomy JF - Journal JO - JOR SP - 72 EP - 78 VL - 11 IS - 2 SN - 1307-8798-1308-8459 M3 - UR - Y2 - 2017 ER -
EndNote %0 Anatomy Chronic anaemia causes degenerative changes in trophoblast cells of the rat placenta %A Omer Awad , Shem J. Ochieng , Abdel Malek , Julius Ogeng’o %T Chronic anaemia causes degenerative changes in trophoblast cells of the rat placenta %D 2017 %J Anatomy %P 1307-8798-1308-8459 %V 11 %N 2 %R %U
ISNAD Awad, Omer , Ochieng, Shem J. , Malek, Abdel , Ogeng’o, Julius . "Chronic anaemia causes degenerative changes in trophoblast cells of the rat placenta". Anatomy 11 / 2 (Ağustos 2017): 72-78.