Yıl 2017, Cilt 23, Sayı 4, Sayfalar 0 - 0 2017-09-16

Determination of Nuclear DNA Content and Ploidy of Hypericum perforatum L. Accessions Collected From Western Turkey

Gülsemin SAVAŞ TUNA [1] , Gözde DUYU [2] , Kardelen UZUN [3] , Gülru YÜCEL [4] , Metin TUNA [5]

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Abstract

Hypericum perforatum L. (St John’s Wort) is a medicinal plant that produces pharmaceutically important compounds with antidepressive, anticancer and antiviral activities. H. perforatum is a facultative apomictic species as it has the ability to reproduce with multiple reproduction mechanisms affecting genetic structure and chemical composition of the plants. 

The objective of this study was to determine nuclear DNA content and ploidy of H. perforatum L. plants growing naturally in the flora of Turkey. The seeds of 39 Hypericum perforatum L. accessions collected from 23 different locations in Turkey were used in the study. Nuclear DNA content of three different seedlings for each of the 39 H. perforatum accessions was determined using flow cytometry. Based on the results of flow cytometric analysis, nuclear DNA content of the accessions varied between 0.8 - 2.57 pg2C-1. Nuclear DNA content differences observed among H. perforatum accessions were statistically significant (P< 0.01) and the Duncan test revealed that the accessions formed three clearly distinguishable main groups. Mean nuclear DNA contents of each group were 0.80 pg2C-1, 1.58 pg2C-1 (1.36-1.73 pg2C-1) and 2.38 pg2C-1 (2.15-2.57 pg2C-1). Ploidy levels were determined by correlating nuclear DNA content of accessions with the number of their mitotic chromosomes by counting chromosome number of one plant from each group.

Findings suggest that H. perforatum accessions used in this study had the following three different ploidy levels: diploid, tetraploid and hexaploid.  The percentage of diploid, tetraploid and hexaploid accessions was 2.2, 86.4 and 11.4% respectively.

Hypericum perforatum, Flow cytometry; Nuclear DNA content; Ploidy level; Medicinal plants
Konular
Dergi Bölümü Makaleler
Yazarlar

Yazar: Gülsemin SAVAŞ TUNA

Yazar: Gözde DUYU

Yazar: Kardelen UZUN

Yazar: Gülru YÜCEL

Yazar: Metin TUNA

Bibtex @ { ankutbd385863, journal = {Journal of Agricultural Sciences}, issn = {}, eissn = {2148-9297}, address = {Ankara Üniversitesi}, year = {2017}, volume = {23}, pages = {0 - 0}, doi = {}, title = {Determination of Nuclear DNA Content and Ploidy of Hypericum perforatum L. Accessions Collected From Western Turkey}, key = {cite}, author = {TUNA, Metin and UZUN, Kardelen and SAVAŞ TUNA, Gülsemin and YÜCEL, Gülru and DUYU, Gözde} }
APA SAVAŞ TUNA, G , DUYU, G , UZUN, K , YÜCEL, G , TUNA, M . (2017). Determination of Nuclear DNA Content and Ploidy of Hypericum perforatum L. Accessions Collected From Western Turkey. Journal of Agricultural Sciences, 23 (4), 0-0. Retrieved from http://dergipark.gov.tr/ankutbd/issue/34822/385863
MLA SAVAŞ TUNA, G , DUYU, G , UZUN, K , YÜCEL, G , TUNA, M . "Determination of Nuclear DNA Content and Ploidy of Hypericum perforatum L. Accessions Collected From Western Turkey". Journal of Agricultural Sciences 23 (2017): 0-0 <http://dergipark.gov.tr/ankutbd/issue/34822/385863>
Chicago SAVAŞ TUNA, G , DUYU, G , UZUN, K , YÜCEL, G , TUNA, M . "Determination of Nuclear DNA Content and Ploidy of Hypericum perforatum L. Accessions Collected From Western Turkey". Journal of Agricultural Sciences 23 (2017): 0-0
RIS TY - JOUR T1 - Determination of Nuclear DNA Content and Ploidy of Hypericum perforatum L. Accessions Collected From Western Turkey AU - Gülsemin SAVAŞ TUNA , Gözde DUYU , Kardelen UZUN , Gülru YÜCEL , Metin TUNA Y1 - 2017 PY - 2017 N1 - DO - T2 - Journal of Agricultural Sciences JF - Journal JO - JOR SP - 0 EP - 0 VL - 23 IS - 4 SN - -2148-9297 M3 - UR - Y2 - 2018 ER -
EndNote %0 Journal of Agricultural Sciences Determination of Nuclear DNA Content and Ploidy of Hypericum perforatum L. Accessions Collected From Western Turkey %A Gülsemin SAVAŞ TUNA , Gözde DUYU , Kardelen UZUN , Gülru YÜCEL , Metin TUNA %T Determination of Nuclear DNA Content and Ploidy of Hypericum perforatum L. Accessions Collected From Western Turkey %D 2017 %J Journal of Agricultural Sciences %P -2148-9297 %V 23 %N 4 %R %U
ISNAD SAVAŞ TUNA, Gülsemin , DUYU, Gözde , UZUN, Kardelen , YÜCEL, Gülru , TUNA, Metin . "Determination of Nuclear DNA Content and Ploidy of Hypericum perforatum L. Accessions Collected From Western Turkey". Journal of Agricultural Sciences 23 / 4 (Eylül 2017): 0-0.