Cilt 5, Sayı 3, Sayfalar 1 - 9 2017-10-01

DNA-binding and spectroscopic studies of cobalt (II) compound containing 2, 10, 16, 24-tetrakis 4-(2-phenoxy-1, 3-dioxo-2, 3-dihydro-1H-inden-2-yloxy) phthalonitrile
2, 10, 16, 24-Tetrakis 4- (2-fenoksi-l, 3-diokso-2,3-dihidro-lH-inden-2-iloksi) ftalonitril içeren kobalt (II) bileşiğinin DNA bağlanmasının spektroskopik yöntemler ile araştırılması

Ali Arslantas [1] , Mehmet Salih Ağırtaş [2]

100 92

In this study, binding activities of the previously synthesized cobalt (II) compound (PcCo) with calf thymus-DNA containing 2, 10, 16, 24-tetrakis 4-(2-phenoxy-1, 3-dioxo-2, 3-dihydro-1H-inden-2-yloxy) phthalonitrile were investigated to determine binding activities by using UV-Vis absorption, fluorescence titration methods. Absorption titration spectra of PcCo in DMF give peaks at about 670 nm for Q-band absorption and about 340 nm for B-band absorption in the absence of calf thymus-DNA. Emission intensities of PcCo in presence of calf thymus-DNA, PcCo gives emission at about 545 nm. Thermal denaturation profile study was systematically studied by UV/Vis spectroscopic technique and melting point temperaturewas found around 7.87 oC for Co (II) phthalocyanine compoundThe reduction oxidation behavior of PcCo with calf thymus-DNA was investigated using cyclic voltammetry (CV). Cathodic peak potential and anodic peak potential for PcCo were recorded to be 0.84, -0.36 V for EPc and 0.88 V, 0.15 V, and -0.69 V EPa. The cyclic voltammetric results show one half- reversible reduction oxidation wave. The shift in Epc and Epa potentials indicates that PcCo shows strong interaction with calf thymus-DNA. Further to understand binding activities of PcCo with calf thymus-DNA was studied by using electrophoresis technique. Electrophoresis findings showed that there is decrease in the intensity of CT-DNA bands. All the findings show that PcCo interacts strongly with DNA molecule via an intercaltion binding.

2,10, 16, 24-tetrakis 4- (2-fenoksi-1,3-diokso-2,3-dihidro-lH-inden-2-iloksi) ftalonitril içeren önceden sentezlenmiş kobalt (II) bileşiğinin (PcCo) CT-DNA ile bağlanma özellikleri UV/Vis absorpsiyon, floresan titrasyonu yöntemleri kullanılarak bağlanma özelliklerini belirlemek için araştırıldı. Termal denatürasyon profili çalışması UV-Vis spektroskopik tekniği ile sistematik olarak incelenmiş ve Co (II) ftalosiyanin bileşiği için erime noktası sıcaklık değeri 7.87 oC civarında bulunmuştur. CT-DNA yokluğunda ve varlığında PcCo'in redüksiyon oksidasyon davranışı, dönüşümlü voltametri (CV) ile incelendi. Dönüşümlü (cyclic) voltametri sonuçlar, bir yarı-tersinir redaksiyon oksidasyon dalgasını göstermektedir. Epc ve Epa potansiyelindeki değişim, PcCo'in CT-DNA'yla güçlü etkileşim gösterdiğini önermektedir. PcCo'in CT-DNA ile olan etkileşimi ayrıca jel elektroforez yöntemi kullanılarak araştırılmıştır. Jel elektroforezi sonuçları, CT-DNA bantlarının yoğunluğunda azalma olduğunu gösterdi. Tüm bulgular, PcCo ile CT-DNA molekülü arasındaki etkileşimin interkalatif bağlanma türü olduğunu göstermektedir.

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Konular Mühendislik ve Temel Bilimler
Dergi Bölümü Makaleler
Yazarlar

Yazar: Ali Arslantas
E-posta: arsoz33@gmail.com
Kurum: Karabük Üniversitesi
Ülke: Turkey


Yazar: Mehmet Salih Ağırtaş
E-posta: salihagirtas@hotmail.com
Kurum: Yüzüncü Yıl Üniversitesi
Ülke: Turkey


Bibtex @araştırma makalesi { apjes329065, journal = {Academic Platform-Journal of Engineering and Science}, issn = {}, address = {Akademik Platform}, year = {2017}, volume = {5}, pages = {1 - 9}, doi = {10.21541/apjes.329065}, title = {DNA-binding and spectroscopic studies of cobalt (II) compound containing 2, 10, 16, 24-tetrakis 4-(2-phenoxy-1, 3-dioxo-2, 3-dihydro-1H-inden-2-yloxy) phthalonitrile}, language = {en}, key = {cite}, author = {Ağırtaş, Mehmet Salih and Arslantas, Ali} } @araştırma makalesi { apjes329065, journal = {Academic Platform-Journal of Engineering and Science}, issn = {}, address = {Akademik Platform}, year = {2017}, volume = {5}, pages = {1 - 9}, doi = {10.21541/apjes.329065}, title = {2, 10, 16, 24-Tetrakis 4- (2-fenoksi-l, 3-diokso-2,3-dihidro-lH-inden-2-iloksi) ftalonitril içeren kobalt (II) bileşiğinin DNA bağlanmasının spektroskopik yöntemler ile araştırılması}, language = {tr}, key = {cite}, author = {Ağırtaş, Mehmet Salih and Arslantas, Ali} }
APA Arslantas, A , Ağırtaş, M . (2017). DNA-binding and spectroscopic studies of cobalt (II) compound containing 2, 10, 16, 24-tetrakis 4-(2-phenoxy-1, 3-dioxo-2, 3-dihydro-1H-inden-2-yloxy) phthalonitrile. Academic Platform-Journal of Engineering and Science, 5 (3), 1-9. DOI: 10.21541/apjes.329065
MLA Arslantas, A , Ağırtaş, M . "DNA-binding and spectroscopic studies of cobalt (II) compound containing 2, 10, 16, 24-tetrakis 4-(2-phenoxy-1, 3-dioxo-2, 3-dihydro-1H-inden-2-yloxy) phthalonitrile". Academic Platform-Journal of Engineering and Science 5 (2017): 1-9 <http://dergipark.gov.tr/apjes/issue/31298/329065>
Chicago Arslantas, A , Ağırtaş, M . "DNA-binding and spectroscopic studies of cobalt (II) compound containing 2, 10, 16, 24-tetrakis 4-(2-phenoxy-1, 3-dioxo-2, 3-dihydro-1H-inden-2-yloxy) phthalonitrile". Academic Platform-Journal of Engineering and Science 5 (2017): 1-9
RIS TY - JOUR T1 - 2, 10, 16, 24-Tetrakis 4- (2-fenoksi-l, 3-diokso-2,3-dihidro-lH-inden-2-iloksi) ftalonitril içeren kobalt (II) bileşiğinin DNA bağlanmasının spektroskopik yöntemler ile araştırılması AU - Ali Arslantas , Mehmet Salih Ağırtaş Y1 - 2017 PY - 2017 N1 - doi: 10.21541/apjes.329065 DO - 10.21541/apjes.329065 T2 - Academic Platform-Journal of Engineering and Science JF - Journal JO - JOR SP - 1 EP - 9 VL - 5 IS - 3 SN - -2147-4575 M3 - doi: 10.21541/apjes.329065 UR - http://dx.doi.org/10.21541/apjes.329065 Y2 - 2017 ER -
EndNote %0 Academic Platform-Journal of Engineering and Science 2, 10, 16, 24-Tetrakis 4- (2-fenoksi-l, 3-diokso-2,3-dihidro-lH-inden-2-iloksi) ftalonitril içeren kobalt (II) bileşiğinin DNA bağlanmasının spektroskopik yöntemler ile araştırılması %A Ali Arslantas , Mehmet Salih Ağırtaş %T 2, 10, 16, 24-Tetrakis 4- (2-fenoksi-l, 3-diokso-2,3-dihidro-lH-inden-2-iloksi) ftalonitril içeren kobalt (II) bileşiğinin DNA bağlanmasının spektroskopik yöntemler ile araştırılması %D 2017 %J Academic Platform-Journal of Engineering and Science %P -2147-4575 %V 5 %N 3 %R doi: 10.21541/apjes.329065 %U 10.21541/apjes.329065