Year 2018, Volume 7, Issue 1, Pages 52 - 62 2018-02-28

Comparative Growth Media Performances on in vitro Propagation of Some Salep Orchids

Yasemin Kemeç Hürkan [1] , Kaan Hürkan [2] , Cüneyt Akı [3]

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Due to the increasing demand and over collection of orchids from nature to produce salep, scientists have been led to search for more efficient ways to propagate these specific orchids in vitro. This present study compares germination performances of two commercial (Orchimax and Knudson C) and one specially prepared orchid growth media (SV), on economically and medicinally important orchids used to make salep; (Anacamptis pyramidalis, Dactylorhiza romana, Neotinea tridentata and Anacamptis morio subsp. morio) and further aims to obtain a mature orchid plant by following the natural environmental cycle.

Significant differences in seed germination and protocorm development were observed. Asymbiotic germination tests showed that the specially prepared growth media performed better than the commercial media by 79.11% germination rate. Also, that A. morio subsp. morio had the best germination rate by 88.91%. Protocorms developed in the sixteenth week after sowing. Soil was collected from the natural habitat of each species and was used as a potting substrate, and this helped orchids to pass their initial acclimatization stage. Regeneration success of orchids at in vitro conditions could be increased by using SV growth medium, following their natural hardening off cycle and using specific substrates from their respective habitats.

Micropropagation, Salep orchids, Orchimax, Knudson C, Svante Malmgren
  • Reference1 Assist. Prof. Dr. BEHİYE BANU BİLGEN Namık Kemal University, Faculty of Agriculture, Department of Agricultural Biotechnology, Campus/ Tekirdağ (0282) 250 - 2291, bbilgen@nku.edu.tr, bbilgen.cv.nku.edu.tr
  • Reference2 Prof. Dr. Aynur GÜREL Ege Univ. Dept of Bioengineering IZMIR aynurgurel@gmail.com, aynur.gurel@ege.edu.tr
  • Reference3 Prof. Dr. Ayşe Filiz GÜREL İstanbul University, Faculty of Science, Dept. of Molecular Biology and Genetics PK 34134 Vezneciler, Fatih/İstanbul - TURKEY Telephone: +90(212) 4555700, Fax: +90(212) 5190834 filiz@istanbul.edu.tr
Primary Language en
Subjects Science
Journal Section Articles
Authors

Author: Yasemin Kemeç Hürkan
Institution: ÇANAKKALE ONSEKİZ MART ÜNİVERSİTESİ, FEN VE EDEBİYAT FAKÜLTESİ, BİYOLOJİ BÖLÜMÜ
Country: Turkey


Author: Kaan Hürkan
Institution: ÇANAKKALE ONSEKİZ MART ÜNİVERSİTESİ, FEN VE EDEBİYAT FAKÜLTESİ, MOLEKÜLER BİYOLOJİ VE GENETİK BÖLÜMÜ
Country: Turkey


Author: Cüneyt Akı
Institution: ÇANAKKALE ONSEKİZ MART ÜNİVERSİTESİ, FEN VE EDEBİYAT FAKÜLTESİ, BİYOLOJİ BÖLÜMÜ
Country: Turkey


Bibtex @research article { aubtdc331328, journal = {ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology}, issn = {2146-0264}, eissn = {2146-0213}, address = {Eskişehir Teknik Üniversitesi}, year = {2018}, volume = {7}, pages = {52 - 62}, doi = {10.18036/aubtdc.331328}, title = {Comparative Growth Media Performances on in vitro Propagation of Some Salep Orchids}, key = {cite}, author = {Kemeç Hürkan, Yasemin and Hürkan, Kaan and Akı, Cüneyt} }
APA Kemeç Hürkan, Y , Hürkan, K , Akı, C . (2018). Comparative Growth Media Performances on in vitro Propagation of Some Salep Orchids. ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology, 7 (1), 52-62. DOI: 10.18036/aubtdc.331328
MLA Kemeç Hürkan, Y , Hürkan, K , Akı, C . "Comparative Growth Media Performances on in vitro Propagation of Some Salep Orchids". ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology 7 (2018): 52-62 <http://dergipark.gov.tr/aubtdc/issue/31126/331328>
Chicago Kemeç Hürkan, Y , Hürkan, K , Akı, C . "Comparative Growth Media Performances on in vitro Propagation of Some Salep Orchids". ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology 7 (2018): 52-62
RIS TY - JOUR T1 - Comparative Growth Media Performances on in vitro Propagation of Some Salep Orchids AU - Yasemin Kemeç Hürkan , Kaan Hürkan , Cüneyt Akı Y1 - 2018 PY - 2018 N1 - doi: 10.18036/aubtdc.331328 DO - 10.18036/aubtdc.331328 T2 - ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology JF - Journal JO - JOR SP - 52 EP - 62 VL - 7 IS - 1 SN - 2146-0264-2146-0213 M3 - doi: 10.18036/aubtdc.331328 UR - https://doi.org/10.18036/aubtdc.331328 Y2 - 2018 ER -
EndNote %0 ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology Comparative Growth Media Performances on in vitro Propagation of Some Salep Orchids %A Yasemin Kemeç Hürkan , Kaan Hürkan , Cüneyt Akı %T Comparative Growth Media Performances on in vitro Propagation of Some Salep Orchids %D 2018 %J ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology %P 2146-0264-2146-0213 %V 7 %N 1 %R doi: 10.18036/aubtdc.331328 %U 10.18036/aubtdc.331328
ISNAD Kemeç Hürkan, Yasemin , Hürkan, Kaan , Akı, Cüneyt . "Comparative Growth Media Performances on in vitro Propagation of Some Salep Orchids". ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology 7 / 1 (February 2018): 52-62. https://doi.org/10.18036/aubtdc.331328