Yıl 2018, Cilt 7, Sayı 1, Sayfalar 52 - 62 2018-02-28

Comparative Growth Media Performances on in vitro Propagation of Some Salep Orchids

Yasemin Kemeç Hürkan [1] , Kaan Hürkan [2] , Cüneyt Akı [3]

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Due to the increasing demand and over collection of orchids from nature to produce salep, scientists have been led to search for more efficient ways to propagate these specific orchids in vitro. This present study compares germination performances of two commercial (Orchimax and Knudson C) and one specially prepared orchid growth media (SV), on economically and medicinally important orchids used to make salep; (Anacamptis pyramidalis, Dactylorhiza romana, Neotinea tridentata and Anacamptis morio subsp. morio) and further aims to obtain a mature orchid plant by following the natural environmental cycle.

Significant differences in seed germination and protocorm development were observed. Asymbiotic germination tests showed that the specially prepared growth media performed better than the commercial media by 79.11% germination rate. Also, that A. morio subsp. morio had the best germination rate by 88.91%. Protocorms developed in the sixteenth week after sowing. Soil was collected from the natural habitat of each species and was used as a potting substrate, and this helped orchids to pass their initial acclimatization stage. Regeneration success of orchids at in vitro conditions could be increased by using SV growth medium, following their natural hardening off cycle and using specific substrates from their respective habitats.

Micropropagation, Salep orchids, Orchimax, Knudson C, Svante Malmgren
  • Reference1 Assist. Prof. Dr. BEHİYE BANU BİLGEN Namık Kemal University, Faculty of Agriculture, Department of Agricultural Biotechnology, Campus/ Tekirdağ (0282) 250 - 2291, bbilgen@nku.edu.tr, bbilgen.cv.nku.edu.tr
  • Reference2 Prof. Dr. Aynur GÜREL Ege Univ. Dept of Bioengineering IZMIR aynurgurel@gmail.com, aynur.gurel@ege.edu.tr
  • Reference3 Prof. Dr. Ayşe Filiz GÜREL İstanbul University, Faculty of Science, Dept. of Molecular Biology and Genetics PK 34134 Vezneciler, Fatih/İstanbul - TURKEY Telephone: +90(212) 4555700, Fax: +90(212) 5190834 filiz@istanbul.edu.tr
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Yazar: Yasemin Kemeç Hürkan
E-posta: kaanhurkan@hotmail.com
Kurum: Çanakkale Onsekiz Mart Üniversitesi, Fen-Edebiyat Fakültesi, Biyoloji Bölümü
Ülke: Turkey


Yazar: Kaan Hürkan
E-posta: kaanhurkan@hotmail.com
Kurum: Çanakkale Onsekiz Mart Üniversitesi, Fen-Edebiyat Fakültesi, Moleküler Biyoloji ve Genetik Bölümü
Ülke: Turkey


Yazar: Cüneyt Akı
E-posta: cuneytaki@comu.edu.tr
Kurum: Çanakkale Onsekiz Mart Üniversitesi, Fen-Edebiyat Fakültesi, Biyoloji Bölümü
Ülke: Turkey


Bibtex @araştırma makalesi { aubtdc331328, journal = {Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji}, issn = {2146-0264}, address = {Anadolu Üniversitesi}, year = {2018}, volume = {7}, pages = {52 - 62}, doi = {10.18036/aubtdc.331328}, title = {Comparative Growth Media Performances on in vitro Propagation of Some Salep Orchids}, key = {cite}, author = {Kemeç Hürkan, Yasemin and Hürkan, Kaan and Akı, Cüneyt} }
APA Kemeç Hürkan, Y , Hürkan, K , Akı, C . (2018). Comparative Growth Media Performances on in vitro Propagation of Some Salep Orchids. Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, 7 (1), 52-62. DOI: 10.18036/aubtdc.331328
MLA Kemeç Hürkan, Y , Hürkan, K , Akı, C . "Comparative Growth Media Performances on in vitro Propagation of Some Salep Orchids". Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 7 (2018): 52-62 <http://dergipark.gov.tr/aubtdc/issue/31126/331328>
Chicago Kemeç Hürkan, Y , Hürkan, K , Akı, C . "Comparative Growth Media Performances on in vitro Propagation of Some Salep Orchids". Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 7 (2018): 52-62
RIS TY - JOUR T1 - Comparative Growth Media Performances on in vitro Propagation of Some Salep Orchids AU - Yasemin Kemeç Hürkan , Kaan Hürkan , Cüneyt Akı Y1 - 2018 PY - 2018 N1 - doi: 10.18036/aubtdc.331328 DO - 10.18036/aubtdc.331328 T2 - Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji JF - Journal JO - JOR SP - 52 EP - 62 VL - 7 IS - 1 SN - 2146-0264-2146-0213 M3 - doi: 10.18036/aubtdc.331328 UR - http://dx.doi.org/10.18036/aubtdc.331328 Y2 - 2018 ER -
EndNote %0 Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji Comparative Growth Media Performances on in vitro Propagation of Some Salep Orchids %A Yasemin Kemeç Hürkan , Kaan Hürkan , Cüneyt Akı %T Comparative Growth Media Performances on in vitro Propagation of Some Salep Orchids %D 2018 %J Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji %P 2146-0264-2146-0213 %V 7 %N 1 %R doi: 10.18036/aubtdc.331328 %U 10.18036/aubtdc.331328