Yıl 2018, Cilt 7, Sayı 2, Sayfalar 130 - 142 2018-08-17

ISOLATION, SCREENING, PARTIAL PURIFICATION AND CHARACTERIZATION OF PROTEASE FROM HALOPHILIC BACTERIA ISOLATED FROM INDONESIAN FERMENTED FOOD

Sarah FITRIANI [1] , Kıymet GÜVEN [2]

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The protease producing bacteria were screened from Indonesian traditional fermented food, tauco and terasi. During the study, 4 halophilic protease producers were isolated from tauco and terasi. Among these isolates, halophilic bacterial isolate TANN 4 was recorded as the best protease producer. Extracellular protease from isolate TANN 4 was partially purified using ammonium sulfate precipitation. The protease was partially purified with final yield of 72.87 % and 25.41 fold purity. This moderate thermoactive and alkaliphilic protease showed a pH optimum of 8.0 and temperature optimum was 50 °C. The enzyme was also active at salt concentrations ranging from 1 to 15 % (w/v), with optimum activity at 1 % NaCl (w/v). Ethylenediaminetetraacetic acid (EDTA) completely inhibited the enzyme activity suggesting that it was a metalloprotease. Among metal ions, the Ca2+, K+ and Mg2+ ions enhanced the activity of enzyme. The KM and Vmax values exhibited by partially purified protease were 0.0649 mM and 216.45 U mg−1 using casein as substrate. The molecular weight was estimated to be 19.8 kDa on SDS PAGE. The enzyme also fairly stable in Triton X-100, SDS, 1 % commercial detergents (OMO and Ariel) and 25 % methanol. This enzyme was capable of hydrolyzing casein, hemoglobin and bovine serum albumin (BSA). Automated ribotyping analysis revealed that 3 isolate (TANN 4, TR 2 and TR 4) resembled Halobacillus trueperi that exhibited 71, 68 and 69 % similarity respectively, and isolate (TR 1) resembled Virgibacillus pantothenticus with 64 % similarity. These characteristics make this halophilic bacterial extracellular metalloprotease seems to be potentially useful for biotechnological and industrial applications.

Metalloprotease, Indonesian fermented food
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Birincil Dil en
Konular Yaşam Bilimleri
Dergi Bölümü Araştırma Makalesi
Yazarlar

Yazar: Sarah FITRIANI
Ülke: Indonesia


Yazar: Kıymet GÜVEN
Ülke: Turkey


Bibtex @araştırma makalesi { aubtdc322711, journal = {Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji}, issn = {2146-0264}, eissn = {2146-0213}, address = {Anadolu Üniversitesi}, year = {2018}, volume = {7}, pages = {130 - 142}, doi = {10.18036/aubtdc.322711}, title = {ISOLATION, SCREENING, PARTIAL PURIFICATION AND CHARACTERIZATION OF PROTEASE FROM HALOPHILIC BACTERIA ISOLATED FROM INDONESIAN FERMENTED FOOD}, key = {cite}, author = {FITRIANI, Sarah and GÜVEN, Kıymet} }
APA FITRIANI, S , GÜVEN, K . (2018). ISOLATION, SCREENING, PARTIAL PURIFICATION AND CHARACTERIZATION OF PROTEASE FROM HALOPHILIC BACTERIA ISOLATED FROM INDONESIAN FERMENTED FOOD. Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, 7 (2), 130-142. DOI: 10.18036/aubtdc.322711
MLA FITRIANI, S , GÜVEN, K . "ISOLATION, SCREENING, PARTIAL PURIFICATION AND CHARACTERIZATION OF PROTEASE FROM HALOPHILIC BACTERIA ISOLATED FROM INDONESIAN FERMENTED FOOD". Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 7 (2018): 130-142 <http://dergipark.gov.tr/aubtdc/issue/36940/322711>
Chicago FITRIANI, S , GÜVEN, K . "ISOLATION, SCREENING, PARTIAL PURIFICATION AND CHARACTERIZATION OF PROTEASE FROM HALOPHILIC BACTERIA ISOLATED FROM INDONESIAN FERMENTED FOOD". Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 7 (2018): 130-142
RIS TY - JOUR T1 - ISOLATION, SCREENING, PARTIAL PURIFICATION AND CHARACTERIZATION OF PROTEASE FROM HALOPHILIC BACTERIA ISOLATED FROM INDONESIAN FERMENTED FOOD AU - Sarah FITRIANI , Kıymet GÜVEN Y1 - 2018 PY - 2018 N1 - doi: 10.18036/aubtdc.322711 DO - 10.18036/aubtdc.322711 T2 - Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji JF - Journal JO - JOR SP - 130 EP - 142 VL - 7 IS - 2 SN - 2146-0264-2146-0213 M3 - doi: 10.18036/aubtdc.322711 UR - http://dx.doi.org/10.18036/aubtdc.322711 Y2 - 2018 ER -
EndNote %0 Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji ISOLATION, SCREENING, PARTIAL PURIFICATION AND CHARACTERIZATION OF PROTEASE FROM HALOPHILIC BACTERIA ISOLATED FROM INDONESIAN FERMENTED FOOD %A Sarah FITRIANI , Kıymet GÜVEN %T ISOLATION, SCREENING, PARTIAL PURIFICATION AND CHARACTERIZATION OF PROTEASE FROM HALOPHILIC BACTERIA ISOLATED FROM INDONESIAN FERMENTED FOOD %D 2018 %J Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji %P 2146-0264-2146-0213 %V 7 %N 2 %R doi: 10.18036/aubtdc.322711 %U 10.18036/aubtdc.322711
ISNAD FITRIANI, Sarah , GÜVEN, Kıymet . "ISOLATION, SCREENING, PARTIAL PURIFICATION AND CHARACTERIZATION OF PROTEASE FROM HALOPHILIC BACTERIA ISOLATED FROM INDONESIAN FERMENTED FOOD". Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 7 / 2 (Ağustos 2018): 130-142. http://dx.doi.org/10.18036/aubtdc.322711