Yıl 2018, Cilt 7, Sayı 2, Sayfalar 105 - 121 2018-08-17

METALLERE VE pH STRESİNE KARŞI Escherichia coli’de RpoS’NİN ROLÜNÜN ARAŞTIRILMASI
Escherichia coli’de Metallere ve pH Stresine karşı RpoS’nin Rolünün Araştırılması

Cihan Darcan [1] , Özge Kaygusuz [2] , Ezgi Aydın [3]

74 111

RpoS (σ38), which acts as an alternative sigma factor in Escherichia coli, controls the expression of genes responsible for cellular responses to different stresses including acidity, starvation, osmotic, oxidative stress, DNA damage, and stationary phase transition. In this study, the role of the rpoS gene in Escherichia coli W3110 under metals and pH stress was researched. The rpoS gene, which is a mutant in the E. coli BW25113 strain, was transferred by transduction with the P1kc phage to wild-type E. coli W3110. Life experiments in wild-type E. coli W3110 and rpoS mutant E. coli W3110 strains were carried out by plaque counting method at 3 different pH, in pH 5.5, 7.0 and 8.0 phosphate buffer. The role of rpoS in the presence of 6 different metals (Zn, Ni, Co, Cd, Ag and Cu) was also determined by the method of dropping petri. Sensitivity at rpoS mutant strains was observed at all pH values studied. It has been determined that the rpoS mutant E. coli against Cd metal shows a very significant resistance, but it has been found that there is no role in other metals. Verification of the role of the rpoS gene has been achieved by genetic complementation. These results show that the RpoS alternative sigma factor plays a role in the control of genes related to the Cd metal and in the genes associated with pH stress.


Escherichia coli’de alternatif sigma faktörü olarak adlandırılan rpoS38) asit şoku, açlık stresi, ozmotik stres, oksidatif stres, DNA hasarı ve durağan faza geçiş dahil olmak üzere farklı streslere karşı hücresel cevapta görevli olan genlerin ekspresyonunu kontrol etmektedir. Bu çalışmada Escherichia coli W3110’da rpoS geninin metal ve pH stresi altındaki rolü araştırılmıştır. E. coli BW25113 suşunda mutant olan rpoS geni, P1kc fajı ile yabani tip E. coli W3110’a aktarılmıştır. Yabani tip E. coli W3110 ve rpoS mutant E. coli W3110 suşunda yaşam deneyleri pH 5.5, 7.0 ve 8.0 fosfat tamponunda olmak üzere 3 farklı pH’da plak sayım metodu ile yapılmıştır. 6 farklı metal varlığında (Zn, Ni, Co, Cd, Ag ve Cu) metal stresindeki rolü minimal inhibisyon konsantrasyonu ve petri damlatma yöntemi ile belirlenmiştir. Çalışılan tüm pH değerlerinde rpoS mutant suşlarda duyarlılık gözlenmiştir. Cd metaline karşı rpoS mutantının oldukça önemli bir direnç gösterdiği belirlenirken, diğer metallerde herhangi bir rolü olmadığı tespit edilmiştir. rpoS geninin rolü, genin tamamlanması yapılarak doğrulaması sağlanmıştır. Bu sonuçlar RpoS alternatif sigma faktörünün Cd metali ile ilişkili genlerin kontrolünde ve pH stresi ile ilişkili genlerde rolü olduğunu göstermektedir

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Yazar: Cihan Darcan (Sorumlu Yazar)
Ülke: Turkey


Yazar: Özge Kaygusuz
Kurum: BİLECİK ŞEYH EDEBALİ ÜNİVERSİTESİ, BİYOTEKNOLOJİ UYGULAMA VE ARAŞTIRMA MERKEZİ
Ülke: Turkey


Yazar: Ezgi Aydın
Kurum: BİLECİK ŞEYH EDEBALİ ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ, MOLEKÜLER BİYOLOJİ VE GENETİK BÖLÜMÜ
Ülke: Turkey


Bibtex @araştırma makalesi { aubtdc372141, journal = {Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji}, issn = {2146-0264}, eissn = {2146-0213}, address = {Eskişehir Teknik Üniversitesi}, year = {2018}, volume = {7}, pages = {105 - 121}, doi = {10.18036/aubtdc.372141}, title = {Escherichia coli’de Metallere ve pH Stresine karşı RpoS’nin Rolünün Araştırılması}, key = {cite}, author = {Kaygusuz, Özge and Aydın, Ezgi and Darcan, Cihan} }
APA Darcan, C , Kaygusuz, Ö , Aydın, E . (2018). Escherichia coli’de Metallere ve pH Stresine karşı RpoS’nin Rolünün Araştırılması. Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, 7 (2), 105-121. DOI: 10.18036/aubtdc.372141
MLA Darcan, C , Kaygusuz, Ö , Aydın, E . "Escherichia coli’de Metallere ve pH Stresine karşı RpoS’nin Rolünün Araştırılması". Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 7 (2018): 105-121 <http://dergipark.gov.tr/aubtdc/issue/36940/372141>
Chicago Darcan, C , Kaygusuz, Ö , Aydın, E . "Escherichia coli’de Metallere ve pH Stresine karşı RpoS’nin Rolünün Araştırılması". Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 7 (2018): 105-121
RIS TY - JOUR T1 - Escherichia coli’de Metallere ve pH Stresine karşı RpoS’nin Rolünün Araştırılması AU - Cihan Darcan , Özge Kaygusuz , Ezgi Aydın Y1 - 2018 PY - 2018 N1 - doi: 10.18036/aubtdc.372141 DO - 10.18036/aubtdc.372141 T2 - Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji JF - Journal JO - JOR SP - 105 EP - 121 VL - 7 IS - 2 SN - 2146-0264-2146-0213 M3 - doi: 10.18036/aubtdc.372141 UR - http://dx.doi.org/10.18036/aubtdc.372141 Y2 - 2018 ER -
EndNote %0 Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji Escherichia coli’de Metallere ve pH Stresine karşı RpoS’nin Rolünün Araştırılması %A Cihan Darcan , Özge Kaygusuz , Ezgi Aydın %T Escherichia coli’de Metallere ve pH Stresine karşı RpoS’nin Rolünün Araştırılması %D 2018 %J Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji %P 2146-0264-2146-0213 %V 7 %N 2 %R doi: 10.18036/aubtdc.372141 %U 10.18036/aubtdc.372141
ISNAD Darcan, Cihan , Kaygusuz, Özge , Aydın, Ezgi . "Escherichia coli’de Metallere ve pH Stresine karşı RpoS’nin Rolünün Araştırılması". Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 7 / 2 (Ağustos 2018): 105-121. http://dx.doi.org/10.18036/aubtdc.372141