Yıl 2018, Cilt 7, Sayı 2, Sayfalar 227 - 238 2018-08-17

PRODUTION OF LIPIDS FROM OLEAGIOUS YEAST CELL IN DIFFERENT CULTURAL TYPES AND MEDIUMS FOR USAGE IN BIODIESEL PRODUCTION
BİYODİZEL ELDESİNDE KULLANILMAK ÜZERE OLEAGİNOUS MAYA HÜCRELERİNDEN FARKLI KÜLTÜR TİPİ VE ORTAMLARDA LİPİT ÜRETİMİ

Seval Cing Yıldırım [1] , Turgay Kanat [2]

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In this study, lipid production from oleaginous yeast Rhodotorula glutinis and biodiesel production from the extracted lipid was performed. In order to determine the maximum lipid production conditions, the effect of various system parameters such as initial pH, temperature, agitation rate, different carbon and nitrogen sources has been examined in batch fermentation system. Media containing molasses and cheese whey were also tested to reduce the cost of biodiesel production. In addition, under optimum conditions, lipid was produced with a two-step fermentation system. Lipid was extracted from culture media according to the modified Bligh and Dyer method and lipids obtained were used for biodiesel production. Analysis of fatty acids and fatty acid methyl esters was carried out on the gas chromatograph-mass spectrometer. As a result of this study, maximum lipid content and biodiesel production were 29.53 ± 0.46% and 80.65%, respectively. It has been determined that fatty acid composition of lipids containing palmitic acid, oleic acid and stearic acid is suitable for biodiesel production.

Bu çalışmada oleginous maya olan Rhodotorula glutinis’ten lipit üretimi ve ekstre edilen lipitten biyodizel üretimi gerçekleştirilmiştir. Maksimum lipit üretim koşullarının belirlenmesi amacıyla başlangıç pH’sı, sıcaklık, çalkalama hızı,  farklı karbon ve azot kaynakları gibi çeşitli sistem paremetrelerinin etkisi kesikli fermentasyon sisteminde incelenmiştir. Biyodizel üretim maliyetini azaltmak için melas ve peynir altı suyu içeren besiyerleri de test edilmiştir. Ayrıca belirlediğimiz optimum koşullarda iki basamaklı fermentasyon sistemiyle lipit üretilmiştir. Kültür ortamlarından lipit ekstraksiyonu modifiye Bligh ve Dyer yöntemine göre gerçekleştirilmiş ve elde edilen lipitler biyodizel üretiminde kullanılmıştır. Yağ asidi ve yağ asidi metil esterlerinin analizi gaz kromatoğrafisi-kütle spektrometresi cihazında yapılmıştır. Bu çalışma sonucunda üretilen maksimum lipit içeriği ve biyodizel verimi sırasıyla  % 29.53±0.46 ve % 80.65 olarak tespit edilmiştir. Palmitik asit, oleik asit ve stearik asit içeren lipitlerin, yağ asidi kompozisyonunun biyodizel üretimine uygun olduğu belirlenmiştir. 

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Birincil Dil tr
Konular Yaşam Bilimleri
Dergi Bölümü Araştırma Makalesi
Yazarlar

Orcid: 0000-0001-7163-9512
Yazar: Seval Cing Yıldırım (Sorumlu Yazar)
Ülke: Turkey


Yazar: Turgay Kanat
Ülke: Turkey


Bibtex @araştırma makalesi { aubtdc397326, journal = {Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji}, issn = {2146-0264}, eissn = {2146-0213}, address = {Anadolu Üniversitesi}, year = {2018}, volume = {7}, pages = {227 - 238}, doi = {10.18036/aubtdc.397326}, title = {BİYODİZEL ELDESİNDE KULLANILMAK ÜZERE OLEAGİNOUS MAYA HÜCRELERİNDEN FARKLI KÜLTÜR TİPİ VE ORTAMLARDA LİPİT ÜRETİMİ}, key = {cite}, author = {Kanat, Turgay and Cing Yıldırım, Seval} }
APA Cing Yıldırım, S , Kanat, T . (2018). BİYODİZEL ELDESİNDE KULLANILMAK ÜZERE OLEAGİNOUS MAYA HÜCRELERİNDEN FARKLI KÜLTÜR TİPİ VE ORTAMLARDA LİPİT ÜRETİMİ. Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, 7 (2), 227-238. DOI: 10.18036/aubtdc.397326
MLA Cing Yıldırım, S , Kanat, T . "BİYODİZEL ELDESİNDE KULLANILMAK ÜZERE OLEAGİNOUS MAYA HÜCRELERİNDEN FARKLI KÜLTÜR TİPİ VE ORTAMLARDA LİPİT ÜRETİMİ". Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 7 (2018): 227-238 <http://dergipark.gov.tr/aubtdc/issue/36940/397326>
Chicago Cing Yıldırım, S , Kanat, T . "BİYODİZEL ELDESİNDE KULLANILMAK ÜZERE OLEAGİNOUS MAYA HÜCRELERİNDEN FARKLI KÜLTÜR TİPİ VE ORTAMLARDA LİPİT ÜRETİMİ". Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 7 (2018): 227-238
RIS TY - JOUR T1 - BİYODİZEL ELDESİNDE KULLANILMAK ÜZERE OLEAGİNOUS MAYA HÜCRELERİNDEN FARKLI KÜLTÜR TİPİ VE ORTAMLARDA LİPİT ÜRETİMİ AU - Seval Cing Yıldırım , Turgay Kanat Y1 - 2018 PY - 2018 N1 - doi: 10.18036/aubtdc.397326 DO - 10.18036/aubtdc.397326 T2 - Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji JF - Journal JO - JOR SP - 227 EP - 238 VL - 7 IS - 2 SN - 2146-0264-2146-0213 M3 - doi: 10.18036/aubtdc.397326 UR - http://dx.doi.org/10.18036/aubtdc.397326 Y2 - 2018 ER -
EndNote %0 Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji BİYODİZEL ELDESİNDE KULLANILMAK ÜZERE OLEAGİNOUS MAYA HÜCRELERİNDEN FARKLI KÜLTÜR TİPİ VE ORTAMLARDA LİPİT ÜRETİMİ %A Seval Cing Yıldırım , Turgay Kanat %T BİYODİZEL ELDESİNDE KULLANILMAK ÜZERE OLEAGİNOUS MAYA HÜCRELERİNDEN FARKLI KÜLTÜR TİPİ VE ORTAMLARDA LİPİT ÜRETİMİ %D 2018 %J Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji %P 2146-0264-2146-0213 %V 7 %N 2 %R doi: 10.18036/aubtdc.397326 %U 10.18036/aubtdc.397326
ISNAD Cing Yıldırım, Seval , Kanat, Turgay . "BİYODİZEL ELDESİNDE KULLANILMAK ÜZERE OLEAGİNOUS MAYA HÜCRELERİNDEN FARKLI KÜLTÜR TİPİ VE ORTAMLARDA LİPİT ÜRETİMİ". Anadolu Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 7 / 2 (Ağustos 2018): 227-238. http://dx.doi.org/10.18036/aubtdc.397326