Yıl 2017, Cilt 7, Sayı 2, Sayfalar 80 - 84 2017-12-26

Determining of Apoptosis at in vitro Conditioned DRG Neurons with TUNEL Method

Elif KAVAL OĞUZ [1]

98 126

In this study, it was aimed to prove the way of in vitro conditioned cells death. There is an increased axonal regeneration after a peripheral nerve is injured if it exposed to an earlier prior injury. The first injury causing the enhanced regeneration is called a conditioning lesion. In this study, Dorsal Root Ganglion primer cell culture was performed then neurons were conditioned in vitro with an effective and new method. It was observed that in vitro conditioned cells were regenerated much faster than control group cells but at the end of 48th hour, they died. The cell death way of these in vitro conditioned neurons was determined by an in situ Cell Detection Kit Fluorescein and visualized by fluorescent microscopy. Finally, apoptotic cell deaths observed at in vitro conditioned neurons. This study is important for the continuity of enhanced regeneration capacity of conditioning lesion effect and neuronal survival and also it will provide the basis for new studies.

Dorsal root ganglion (DRG), regeneration, conditioning lesion
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Yazarlar

Yazar: Elif KAVAL OĞUZ
Kurum: YÜZÜNCÜ YIL ÜNİVERSİTESİ
Ülke: Turkey


Bibtex @araştırma makalesi { beuscitech331471, journal = {Bitlis Eren University Journal of Science and Technology}, issn = {}, eissn = {2146-7706}, address = {Bitlis Eren Üniversitesi}, year = {2017}, volume = {7}, pages = {80 - 84}, doi = {10.17678/beuscitech.331471}, title = {Determining of Apoptosis at in vitro Conditioned DRG Neurons with TUNEL Method}, key = {cite}, author = {KAVAL OĞUZ, Elif} }
APA KAVAL OĞUZ, E . (2017). Determining of Apoptosis at in vitro Conditioned DRG Neurons with TUNEL Method. Bitlis Eren University Journal of Science and Technology, 7 (2), 80-84. DOI: 10.17678/beuscitech.331471
MLA KAVAL OĞUZ, E . "Determining of Apoptosis at in vitro Conditioned DRG Neurons with TUNEL Method". Bitlis Eren University Journal of Science and Technology 7 (2017): 80-84 <http://dergipark.gov.tr/beuscitech/issue/32937/331471>
Chicago KAVAL OĞUZ, E . "Determining of Apoptosis at in vitro Conditioned DRG Neurons with TUNEL Method". Bitlis Eren University Journal of Science and Technology 7 (2017): 80-84
RIS TY - JOUR T1 - Determining of Apoptosis at in vitro Conditioned DRG Neurons with TUNEL Method AU - Elif KAVAL OĞUZ Y1 - 2017 PY - 2017 N1 - doi: 10.17678/beuscitech.331471 DO - 10.17678/beuscitech.331471 T2 - Bitlis Eren University Journal of Science and Technology JF - Journal JO - JOR SP - 80 EP - 84 VL - 7 IS - 2 SN - -2146-7706 M3 - doi: 10.17678/beuscitech.331471 UR - http://dx.doi.org/10.17678/beuscitech.331471 Y2 - 2017 ER -
EndNote %0 Bitlis Eren University Journal of Science and Technology Determining of Apoptosis at in vitro Conditioned DRG Neurons with TUNEL Method %A Elif KAVAL OĞUZ %T Determining of Apoptosis at in vitro Conditioned DRG Neurons with TUNEL Method %D 2017 %J Bitlis Eren University Journal of Science and Technology %P -2146-7706 %V 7 %N 2 %R doi: 10.17678/beuscitech.331471 %U 10.17678/beuscitech.331471
ISNAD KAVAL OĞUZ, Elif . "Determining of Apoptosis at in vitro Conditioned DRG Neurons with TUNEL Method". Bitlis Eren University Journal of Science and Technology 7 / 2 (Aralık 2017): 80-84. http://dx.doi.org/10.17678/beuscitech.331471