Yıl 2017, Cilt 13, Sayı 4, Sayfalar 833 - 837 2017-12-29

Determination of Enzyme Profiles and Molecular Characterization of Yeast Species Isolated from Butter Samples

Cengiz Çorbacı [1] , Ebru Uyar [2] , Hüsniye Tansel Yalçın [3]

209 187

Constituting the most important group of microorganisms that offered to human consumption through food, yeasts are a substantial part of microflora in dairy products. Due to sophisticated enzyme production especially high proteolytic and lipolytic activities, some of the yeast species play an essential role in many processes such as fermentation, ripening, production of aroma precursors and deterioration in dairy product indicating these products as a source of extracellular enzyme producer yeast strains.  In this study, butter samples were collected from delicatessens and grocery stores in different locations in İzmir province in order to isolate enzyme producers. A total of 40 yeast strains were isolated and investigated for their lipase, protease and amylase activities on solid medium consisting of tributyrin, skim milk and starch, respectively. Sixteen yeast isolates were found to be positive for enzyme production. Lipase and protease activities were observed in 11 yeast strains (68,75%), while only one yeast strain (6,25%) was found to be positive for protease and amylase production. 4 of yeast strains (25%) were able to produce only lipase enzyme. Molecular characterization of the yeast strains based on 26S rRNA region D1/D2 domains revealed that the isolates belonged to Candida zeylanoides (62,5%) and Pichia fermentas (37,5%) species. 

Amylase, identification, isolation, lipase, yeast, protease, 26S rRNA sequencing
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Konular Mühendislik ve Temel Bilimler
Dergi Bölümü Makaleler
Yazarlar

Yazar: Cengiz Çorbacı

Yazar: Ebru Uyar

Yazar: Hüsniye Tansel Yalçın

Bibtex @araştırma makalesi { cbayarfbe370359, journal = {Celal Bayar Üniversitesi Fen Bilimleri Dergisi}, issn = {1305-130X}, eissn = {1305-1385}, address = {Celal Bayar Üniversitesi}, year = {2017}, volume = {13}, pages = {833 - 837}, doi = {10.18466/cbayarfbe.370359}, title = {Determination of Enzyme Profiles and Molecular Characterization of Yeast Species Isolated from Butter Samples}, key = {cite}, author = {Yalçın, Hüsniye Tansel and Uyar, Ebru and Çorbacı, Cengiz} }
APA Çorbacı, C , Uyar, E , Yalçın, H . (2017). Determination of Enzyme Profiles and Molecular Characterization of Yeast Species Isolated from Butter Samples. Celal Bayar Üniversitesi Fen Bilimleri Dergisi, 13 (4), 833-837. Retrieved from http://dergipark.gov.tr/cbayarfbe/issue/33464/370359
MLA Çorbacı, C , Uyar, E , Yalçın, H . "Determination of Enzyme Profiles and Molecular Characterization of Yeast Species Isolated from Butter Samples". Celal Bayar Üniversitesi Fen Bilimleri Dergisi 13 (2017): 833-837 <http://dergipark.gov.tr/cbayarfbe/issue/33464/370359>
Chicago Çorbacı, C , Uyar, E , Yalçın, H . "Determination of Enzyme Profiles and Molecular Characterization of Yeast Species Isolated from Butter Samples". Celal Bayar Üniversitesi Fen Bilimleri Dergisi 13 (2017): 833-837
RIS TY - JOUR T1 - Determination of Enzyme Profiles and Molecular Characterization of Yeast Species Isolated from Butter Samples AU - Cengiz Çorbacı , Ebru Uyar , Hüsniye Tansel Yalçın Y1 - 2017 PY - 2017 N1 - DO - T2 - Celal Bayar Üniversitesi Fen Bilimleri Dergisi JF - Journal JO - JOR SP - 833 EP - 837 VL - 13 IS - 4 SN - 1305-130X-1305-1385 M3 - UR - Y2 - 2017 ER -
EndNote %0 Celal Bayar Üniversitesi Fen Bilimleri Dergisi Determination of Enzyme Profiles and Molecular Characterization of Yeast Species Isolated from Butter Samples %A Cengiz Çorbacı , Ebru Uyar , Hüsniye Tansel Yalçın %T Determination of Enzyme Profiles and Molecular Characterization of Yeast Species Isolated from Butter Samples %D 2017 %J Celal Bayar Üniversitesi Fen Bilimleri Dergisi %P 1305-130X-1305-1385 %V 13 %N 4 %R %U
ISNAD Çorbacı, Cengiz , Uyar, Ebru , Yalçın, Hüsniye Tansel . "Determination of Enzyme Profiles and Molecular Characterization of Yeast Species Isolated from Butter Samples". Celal Bayar Üniversitesi Fen Bilimleri Dergisi 13 / 4 (Aralık 2017): 833-837.