Yıl 2018, Cilt 14, Sayı 1, Sayfalar 35 - 40 2018-03-30

Ethanol Extract of Pinus nigra ssp. pallasiana var. şeneriana Inhibits Human Breast Cancer Cell Viability through Induction of Apoptosis

Harika Atmaca [1] , Çisil Çamlı [2] , Seda Sert [3]

81 85

Novel natural compounds became the new promising source for alternative drug production for cancer treatment. Several studies have reported that different species of pine genus show cytotoxic activities against various cancer cell lines. In the present study, the possible cytotoxic and apoptotic effects of the endemic Pinus nigra ssp. pallasiana var. şeneriana (PS) extract in MCF-7 breast cancer cells were evaluated. Cell viability was determined via XTT assay and verified by live/dead viability/cytotoxicity assay. Apoptotic effect was investigated by mitochondrial membrane potential (MMP) assay. Expression of apoptosis related proteins after treatment with PS extract was detected by apoptosis array. PS extract treatment reduced the viability of MCF-7 cells in a time and concentration-dependent manner. Apoptosis was induced and MMP was decreased in MCF-7 cells by 48 h treatment. Bcl-2, cIAP-1, cIAP-2, Survivin and XIAP protein levels were reduced by 2.8-, 3.2-, 2.8-, 2.5- and 2.2- fold, and Bad, Bax, Cytochrome c and SMAC/Diablo protein levels were induced by 4.2-, 3.0-, 3.6-, and 4.0- fold in MCF-7 cells by PS extract treatment. This report describes the cytotoxic and apoptotic effects of the extract of endemic Pinus species on MCF-7 cells for the first time. Although the isolation and structure elucidation of the active compounds is needed to be performed in the further studies, it is likely that PS extract may be a potential candidate for the development of novel therapeutic anticancer compounds for breast cancer.

Apoptosis, breast cancer, cytotoxicity, endemic, MCF-7, Pinus nigra
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Birincil Dil en
Konular Mühendislik ve Temel Bilimler
Dergi Bölümü Makaleler
Yazarlar

Yazar: Harika Atmaca
Ülke: Turkey


Yazar: Çisil Çamlı
Ülke: Turkey


Yazar: Seda Sert
Ülke: Turkey


Bibtex @araştırma makalesi { cbayarfbe334883, journal = {Celal Bayar Üniversitesi Fen Bilimleri Dergisi}, issn = {1305-130X}, eissn = {1305-1385}, address = {Celal Bayar Üniversitesi}, year = {2018}, volume = {14}, pages = {35 - 40}, doi = {10.18466/cbayarfbe.334883}, title = {Ethanol Extract of Pinus nigra ssp. pallasiana var. şeneriana Inhibits Human Breast Cancer Cell Viability through Induction of Apoptosis}, key = {cite}, author = {Çamlı, Çisil and Sert, Seda and Atmaca, Harika} }
APA Atmaca, H , Çamlı, Ç , Sert, S . (2018). Ethanol Extract of Pinus nigra ssp. pallasiana var. şeneriana Inhibits Human Breast Cancer Cell Viability through Induction of Apoptosis. Celal Bayar Üniversitesi Fen Bilimleri Dergisi, 14 (1), 35-40. DOI: 10.18466/cbayarfbe.334883
MLA Atmaca, H , Çamlı, Ç , Sert, S . "Ethanol Extract of Pinus nigra ssp. pallasiana var. şeneriana Inhibits Human Breast Cancer Cell Viability through Induction of Apoptosis". Celal Bayar Üniversitesi Fen Bilimleri Dergisi 14 (2018): 35-40 <http://dergipark.gov.tr/cbayarfbe/issue/36240/334883>
Chicago Atmaca, H , Çamlı, Ç , Sert, S . "Ethanol Extract of Pinus nigra ssp. pallasiana var. şeneriana Inhibits Human Breast Cancer Cell Viability through Induction of Apoptosis". Celal Bayar Üniversitesi Fen Bilimleri Dergisi 14 (2018): 35-40
RIS TY - JOUR T1 - Ethanol Extract of Pinus nigra ssp. pallasiana var. şeneriana Inhibits Human Breast Cancer Cell Viability through Induction of Apoptosis AU - Harika Atmaca , Çisil Çamlı , Seda Sert Y1 - 2018 PY - 2018 N1 - doi: 10.18466/cbayarfbe.334883 DO - 10.18466/cbayarfbe.334883 T2 - Celal Bayar Üniversitesi Fen Bilimleri Dergisi JF - Journal JO - JOR SP - 35 EP - 40 VL - 14 IS - 1 SN - 1305-130X-1305-1385 M3 - doi: 10.18466/cbayarfbe.334883 UR - http://dx.doi.org/10.18466/cbayarfbe.334883 Y2 - 2018 ER -
EndNote %0 Celal Bayar Üniversitesi Fen Bilimleri Dergisi Ethanol Extract of Pinus nigra ssp. pallasiana var. şeneriana Inhibits Human Breast Cancer Cell Viability through Induction of Apoptosis %A Harika Atmaca , Çisil Çamlı , Seda Sert %T Ethanol Extract of Pinus nigra ssp. pallasiana var. şeneriana Inhibits Human Breast Cancer Cell Viability through Induction of Apoptosis %D 2018 %J Celal Bayar Üniversitesi Fen Bilimleri Dergisi %P 1305-130X-1305-1385 %V 14 %N 1 %R doi: 10.18466/cbayarfbe.334883 %U 10.18466/cbayarfbe.334883
ISNAD Atmaca, Harika , Çamlı, Çisil , Sert, Seda . "Ethanol Extract of Pinus nigra ssp. pallasiana var. şeneriana Inhibits Human Breast Cancer Cell Viability through Induction of Apoptosis". Celal Bayar Üniversitesi Fen Bilimleri Dergisi 14 / 1 (Mart 2018): 35-40. http://dx.doi.org/10.18466/cbayarfbe.334883