Yıl 2018, Cilt 14, Sayı 3, Sayfalar 351 - 355 2018-09-30

Comparison of the Isolation Methods of Viral Nucleic Acids

Nagehan Şakrucu [1] , Uğur Sıdal [2] , Tamer Şanlıdağ [3]

18 30

In vitro amplification of the nucleic acids (DNA or RNA) is used in the detection of microbial agents and thus in the diagnosis of infectious diseases, as well as in the diagnoses of oncological and genetic disorders and forensic medicine. The aim of the present study was to compare the isolation methods of the nucleic acids of hepatitis B and C viruses, causative agents of the two significant infections worldwide. Conventional isolation methods were compared with the commercial kits that have been used commonly in recent years, in terms of reliability, cost-effectiveness, contamination risk and duration of the testing time. Five standards for the isolation of the viral nucleic acids of both HBV DNA (Fluorion HBV QNP 2.0) and HCV RNA (Fluorion HCV QNP 2.1) were used. The isolations of the viral nucleic acids of HBV and HCV were done with the conventional methods, phenol-chloroform and guanidine thiocyanate, and the commercial kits Roboscreen and NucleoSpin. The resultant viral nucleic acid load was determined with a spectrophotometer (WPA UV 1101, Biotech Photometer), and their amplification was conducted with Real-Time PCR. The results of the assessments revealed that the highest nucleic acid concentration were obtained with the conventional methods, while they exhibited significant drawbacks such as long duration of the testing time, difficulty in application, and higher contamination risk.

HBV, HCV, PCR, spectrophotometer, amplification.
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Birincil Dil en
Konular Mühendislik
Dergi Bölümü Makaleler
Yazarlar

Yazar: Nagehan Şakrucu

Yazar: Uğur Sıdal
Kurum: Manisa Celal Bayar University, Faculty of Science and Arts, Department of Biology, Manisa, Turkey
Ülke: Turkey


Yazar: Tamer Şanlıdağ
Kurum: Manisa Celal Bayar University, Faculty of Medicine, Department of Microbiology, Manisa, Turkey
Ülke: Turkey


Bibtex @araştırma makalesi { cbayarfbe350005, journal = {Celal Bayar Üniversitesi Fen Bilimleri Dergisi}, issn = {1305-130X}, eissn = {1305-1385}, address = {Celal Bayar Üniversitesi}, year = {2018}, volume = {14}, pages = {351 - 355}, doi = {10.18466/cbayarfbe.350005}, title = {Comparison of the Isolation Methods of Viral Nucleic Acids}, key = {cite}, author = {Sıdal, Uğur and Şanlıdağ, Tamer and Şakrucu, Nagehan} }
APA Şakrucu, N , Sıdal, U , Şanlıdağ, T . (2018). Comparison of the Isolation Methods of Viral Nucleic Acids. Celal Bayar Üniversitesi Fen Bilimleri Dergisi, 14 (3), 351-355. DOI: 10.18466/cbayarfbe.350005
MLA Şakrucu, N , Sıdal, U , Şanlıdağ, T . "Comparison of the Isolation Methods of Viral Nucleic Acids". Celal Bayar Üniversitesi Fen Bilimleri Dergisi 14 (2018): 351-355 <http://dergipark.gov.tr/cbayarfbe/issue/39486/350005>
Chicago Şakrucu, N , Sıdal, U , Şanlıdağ, T . "Comparison of the Isolation Methods of Viral Nucleic Acids". Celal Bayar Üniversitesi Fen Bilimleri Dergisi 14 (2018): 351-355
RIS TY - JOUR T1 - Comparison of the Isolation Methods of Viral Nucleic Acids AU - Nagehan Şakrucu , Uğur Sıdal , Tamer Şanlıdağ Y1 - 2018 PY - 2018 N1 - doi: 10.18466/cbayarfbe.350005 DO - 10.18466/cbayarfbe.350005 T2 - Celal Bayar Üniversitesi Fen Bilimleri Dergisi JF - Journal JO - JOR SP - 351 EP - 355 VL - 14 IS - 3 SN - 1305-130X-1305-1385 M3 - doi: 10.18466/cbayarfbe.350005 UR - http://dx.doi.org/10.18466/cbayarfbe.350005 Y2 - 2018 ER -
EndNote %0 Celal Bayar Üniversitesi Fen Bilimleri Dergisi Comparison of the Isolation Methods of Viral Nucleic Acids %A Nagehan Şakrucu , Uğur Sıdal , Tamer Şanlıdağ %T Comparison of the Isolation Methods of Viral Nucleic Acids %D 2018 %J Celal Bayar Üniversitesi Fen Bilimleri Dergisi %P 1305-130X-1305-1385 %V 14 %N 3 %R doi: 10.18466/cbayarfbe.350005 %U 10.18466/cbayarfbe.350005
ISNAD Şakrucu, Nagehan , Sıdal, Uğur , Şanlıdağ, Tamer . "Comparison of the Isolation Methods of Viral Nucleic Acids". Celal Bayar Üniversitesi Fen Bilimleri Dergisi 14 / 3 (Eylül 2018): 351-355. http://dx.doi.org/10.18466/cbayarfbe.350005