Yıl 2018, Cilt 14, Sayı 4, Sayfalar 405 - 412 2018-12-28

Optimization of Cryopreservation Process Using Response Surface Methodology for Chlorella saccharophila and Chlorella zofingiensis

Zeliha Demirel [1] , Esra Imamoglu [2] , İrem Deniz [3] , Meltem Conk Dalay [4]

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The preservation of microalgae in a stable state is a fundamental requirement in pharmaceutical, agricultural, environmental sciences and different industries. Cryopreservation is widely stabilized for achieving long-term storage and has been applied to an increasingly diverse range of microalgae and cell cultures. The continuous storage of actively growing microalgae strains by routine serial subculture is relatively time-consuming and this technique has possible contamination risks. In this study, the optimization of cryopreservation process was carried out for two different Chlorella strains using response surface methodology (RSM) with three factors (cryoprotectant concentration, incubation time and cryopreservation time) including 19 runs. The optimal cell viability of C. zofingiensis was found at the dimethyl sulfoxide (DMSO) concentration of 12.89% at the incubation time of 8.14 min and with the cryopreservation time of 93.45 day, while C. saccharophila was found at the DMSO concentration of 12.86 % at the incubation time of 7.99 min and at cryopreservation time of 95.17 day.

Cryopreservation, response surface methodology, Chlorella saccharophila, Chlorella zofingiensis, response surface methodology
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Birincil Dil en
Konular Mühendislik
Yayımlanma Tarihi 31-12-2018
Dergi Bölümü Makaleler
Yazarlar

Yazar: Zeliha Demirel (Sorumlu Yazar)
Kurum: EGE ÜNİVERSİTESİ, MÜHENDİSLİK FAKÜLTESİ
Ülke: Turkey


Yazar: Esra Imamoglu
Kurum: EGE ÜNİVERSİTESİ, MÜHENDİSLİK FAKÜLTESİ
Ülke: Turkey


Yazar: İrem Deniz
Kurum: MANİSA CELÂL BAYAR ÜNİVERSİTESİ
Ülke: Turkey


Yazar: Meltem Conk Dalay
Kurum: EGE ÜNİVERSİTESİ, MÜHENDİSLİK FAKÜLTESİ
Ülke: Turkey


Bibtex @araştırma makalesi { cbayarfbe426444, journal = {Celal Bayar Üniversitesi Fen Bilimleri Dergisi}, issn = {1305-130X}, eissn = {1305-1385}, address = {Celal Bayar Üniversitesi}, year = {2018}, volume = {14}, pages = {405 - 412}, doi = {10.18466/cbayarfbe.426444}, title = {Optimization of Cryopreservation Process Using Response Surface Methodology for Chlorella saccharophila and Chlorella zofingiensis}, key = {cite}, author = {Deniz, İrem and Demirel, Zeliha and Conk Dalay, Meltem and Imamoglu, Esra} }
APA Demirel, Z , Imamoglu, E , Deniz, İ , Conk Dalay, M . (2018). Optimization of Cryopreservation Process Using Response Surface Methodology for Chlorella saccharophila and Chlorella zofingiensis. Celal Bayar Üniversitesi Fen Bilimleri Dergisi, 14 (4), 405-412. DOI: 10.18466/cbayarfbe.426444
MLA Demirel, Z , Imamoglu, E , Deniz, İ , Conk Dalay, M . "Optimization of Cryopreservation Process Using Response Surface Methodology for Chlorella saccharophila and Chlorella zofingiensis". Celal Bayar Üniversitesi Fen Bilimleri Dergisi 14 (2018): 405-412 <http://dergipark.gov.tr/cbayarfbe/issue/41718/426444>
Chicago Demirel, Z , Imamoglu, E , Deniz, İ , Conk Dalay, M . "Optimization of Cryopreservation Process Using Response Surface Methodology for Chlorella saccharophila and Chlorella zofingiensis". Celal Bayar Üniversitesi Fen Bilimleri Dergisi 14 (2018): 405-412
RIS TY - JOUR T1 - Optimization of Cryopreservation Process Using Response Surface Methodology for Chlorella saccharophila and Chlorella zofingiensis AU - Zeliha Demirel , Esra Imamoglu , İrem Deniz , Meltem Conk Dalay Y1 - 2018 PY - 2018 N1 - doi: 10.18466/cbayarfbe.426444 DO - 10.18466/cbayarfbe.426444 T2 - Celal Bayar Üniversitesi Fen Bilimleri Dergisi JF - Journal JO - JOR SP - 405 EP - 412 VL - 14 IS - 4 SN - 1305-130X-1305-1385 M3 - doi: 10.18466/cbayarfbe.426444 UR - http://dx.doi.org/10.18466/cbayarfbe.426444 Y2 - 2018 ER -
EndNote %0 Celal Bayar Üniversitesi Fen Bilimleri Dergisi Optimization of Cryopreservation Process Using Response Surface Methodology for Chlorella saccharophila and Chlorella zofingiensis %A Zeliha Demirel , Esra Imamoglu , İrem Deniz , Meltem Conk Dalay %T Optimization of Cryopreservation Process Using Response Surface Methodology for Chlorella saccharophila and Chlorella zofingiensis %D 2018 %J Celal Bayar Üniversitesi Fen Bilimleri Dergisi %P 1305-130X-1305-1385 %V 14 %N 4 %R doi: 10.18466/cbayarfbe.426444 %U 10.18466/cbayarfbe.426444
ISNAD Demirel, Zeliha , Imamoglu, Esra , Deniz, İrem , Conk Dalay, Meltem . "Optimization of Cryopreservation Process Using Response Surface Methodology for Chlorella saccharophila and Chlorella zofingiensis". Celal Bayar Üniversitesi Fen Bilimleri Dergisi 14 / 4 (Aralık 2018): 405-412. http://dx.doi.org/10.18466/cbayarfbe.426444