Yıl 2018, Cilt 14, Sayı 4, Sayfalar 449 - 454 2018-12-28

Papain Immobilization on NiFe2O4 Magnetic Nanoparticles Functionalized with Gallic Acid and Microwave Assisted Digestion of Bovine Serum Albumin

Nuray Güy [1]

10 43

NiFe2O4 magnetic nanoparticles were solvothermally prepared. NiFe2O4 magnetic nanoparticles were functionalized with gallic acid and then papain immobilized on NiFe2O4 functionalized with gallic acid. The obtained samples were characterized and the activities of free and immobilized papain were studied. Immobilized papain showed higher and more effective activity than free papain. This immobilized papain retained about 75.5% of its initial activity after 8 weeks of storage at 4 °C in sodium phosphate buffer (0.1 M, pH 6.5), while the free trypsin protected 30.5% of its initial activity during the same condition. Furthermore, the immobilized papain protected approximately 51% of its initial activity following the times of ten sequential reuse. Finally, the microwave assisted digestion of bovine serum albumin was carried out for 15 s with matrix-assisted laser desorption/ionization mass spectrometry by using free and immobilized papain.

NiFe2O4, gallic acid, papain, immobilization, protein digestion
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Birincil Dil en
Konular Mühendislik
Yayımlanma Tarihi 31-12-2018
Dergi Bölümü Makaleler
Yazarlar

Yazar: Nuray Güy (Sorumlu Yazar)
Kurum: SAKARYA ÜNİVERSİTESİ
Ülke: Turkey


Bibtex @araştırma makalesi { cbayarfbe453636, journal = {Celal Bayar Üniversitesi Fen Bilimleri Dergisi}, issn = {1305-130X}, eissn = {1305-1385}, address = {Celal Bayar Üniversitesi}, year = {2018}, volume = {14}, pages = {449 - 454}, doi = {10.18466/cbayarfbe.453636}, title = {Papain Immobilization on NiFe2O4 Magnetic Nanoparticles Functionalized with Gallic Acid and Microwave Assisted Digestion of Bovine Serum Albumin}, key = {cite}, author = {Güy, Nuray} }
APA Güy, N . (2018). Papain Immobilization on NiFe2O4 Magnetic Nanoparticles Functionalized with Gallic Acid and Microwave Assisted Digestion of Bovine Serum Albumin. Celal Bayar Üniversitesi Fen Bilimleri Dergisi, 14 (4), 449-454. DOI: 10.18466/cbayarfbe.453636
MLA Güy, N . "Papain Immobilization on NiFe2O4 Magnetic Nanoparticles Functionalized with Gallic Acid and Microwave Assisted Digestion of Bovine Serum Albumin". Celal Bayar Üniversitesi Fen Bilimleri Dergisi 14 (2018): 449-454 <http://dergipark.gov.tr/cbayarfbe/issue/41718/453636>
Chicago Güy, N . "Papain Immobilization on NiFe2O4 Magnetic Nanoparticles Functionalized with Gallic Acid and Microwave Assisted Digestion of Bovine Serum Albumin". Celal Bayar Üniversitesi Fen Bilimleri Dergisi 14 (2018): 449-454
RIS TY - JOUR T1 - Papain Immobilization on NiFe2O4 Magnetic Nanoparticles Functionalized with Gallic Acid and Microwave Assisted Digestion of Bovine Serum Albumin AU - Nuray Güy Y1 - 2018 PY - 2018 N1 - doi: 10.18466/cbayarfbe.453636 DO - 10.18466/cbayarfbe.453636 T2 - Celal Bayar Üniversitesi Fen Bilimleri Dergisi JF - Journal JO - JOR SP - 449 EP - 454 VL - 14 IS - 4 SN - 1305-130X-1305-1385 M3 - doi: 10.18466/cbayarfbe.453636 UR - http://dx.doi.org/10.18466/cbayarfbe.453636 Y2 - 2018 ER -
EndNote %0 Celal Bayar Üniversitesi Fen Bilimleri Dergisi Papain Immobilization on NiFe2O4 Magnetic Nanoparticles Functionalized with Gallic Acid and Microwave Assisted Digestion of Bovine Serum Albumin %A Nuray Güy %T Papain Immobilization on NiFe2O4 Magnetic Nanoparticles Functionalized with Gallic Acid and Microwave Assisted Digestion of Bovine Serum Albumin %D 2018 %J Celal Bayar Üniversitesi Fen Bilimleri Dergisi %P 1305-130X-1305-1385 %V 14 %N 4 %R doi: 10.18466/cbayarfbe.453636 %U 10.18466/cbayarfbe.453636
ISNAD Güy, Nuray . "Papain Immobilization on NiFe2O4 Magnetic Nanoparticles Functionalized with Gallic Acid and Microwave Assisted Digestion of Bovine Serum Albumin". Celal Bayar Üniversitesi Fen Bilimleri Dergisi 14 / 4 (Aralık 2018): 449-454. http://dx.doi.org/10.18466/cbayarfbe.453636