Yıl 2018, Cilt 14, Sayı 4, Sayfalar 455 - 460 2018-12-28

Visualization of Human Kidney Embryonic Cells by Acoustic Microscopy

Bükem Bilen [1] , Nural Pastaci Ozsobaci [2] , Fatma Ates Alkan [3] , Mehmet Burcin Unku [4]

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The aim of our study is to evaluate the capability of acoustic microscopy with a high frequency transducer for visualization of cells. Human kidney embryonic (HEK293) cells were observed by scanning acoustic microscope (SAM). HEK293 cells cultured on cover glasses were kept in cell culture plates. A transducer with 320 MHz center frequency and a spot size of 4.0 µm was incorporated into SAM. Two-dimensional acoustic images were obtained successfully to identify the morphology and the acoustic properties of HEK293 cells and the acoustic impedance of HEK293 cells was measured as 1.65 ± 0.02 MRayl. The same cells on cover glasses were observed also with inverted microscope. The results in this study indicate that scanning acoustic microscopy can be used for the determination of elastic properties of cells, therefore for cellular mapping and molecular imaging, since it can create very sharp images of HEK293 cells with a high frequency transducer.

Scanning acoustic microscopy, kidney cell, acoustic impedance
  • 1. Ziemann, F, Radler, J, Sackmann, E, Local Measurements of Viscoelastic Moduli of Entangled Actin Network Using an Oscillating Magnetic Bead Micro-Rheometer, Biophysical Journal, 1994, 66, 2210–2216.
  • 2. Schmid-Schönbein, GW, Sung, KLP, Tozeren, H, Skalak, R, Chien, S, Passive mechanical properties of human leukocytes, Biophysical Journal, 1981, 36, 243-256.
  • 3. Hochmuth, RM, Micropipette aspiration of living cells, Journal of Biomechanics, 2000, 33, 15-22.
  • 4. Van Vliet, KJ, Bao, G, Suresh, S, The biomechanics toolbox: experimental approaches for living cells and biomolecules, Acta Materialia, 2003, 51, 5881–5905.
  • 5. Laurent, VM, Henon, S, Planus, E, Fodil, R, Balland, M, Isabey, D, Gallet, F, Assessment of Mechanical Properties of Adherent Living Cells by Bead Micromanipulation: Comparison of Magnetic Twisting Cytometry vs Optical Tweezers, Journal of Biomechanical Engineering, 2002, 124, 408–421.
  • 6. Miura, K, Egawa, Y, Moriki, T, Mineta, H, Harada, H, Baba, S, Yamamoto, S, Microscopic observation of chemical modification in sections using scanning acoustic microscopy, Pathology International, 2015, 65, 355-66.
  • 7. Miura, K, Nasu, H, Yamamoto, S, Scanning acoustic microscopy for characterization of neoplastic and inflammatory lesions of lymph nodes, Scientific Reports, 2013, 3, 1255.
  • 8. Brewin, MP, Srodon, PD, Greenwald, SE, Birch, MJ, Carotid atherosclerotic plaque characterization by measurement of ultrasound speed in vitro at high frequency, 20 MHz, Ultrasonics, 2014, 54, 428-441.
  • 9. Kobayashi, K, Yoshida, S, Saijo, Y, Hozumi, N, Acoustic impedance microscopy for biological tissue characterization, Ultrasonics, 2014, 54, 1922-1928.
  • 10. Johnston, RN, Atalar, A, Heiserman, J, Jipson, V, Quate, CF, Acoustic microscopy: Resolution of subcellular detail, Proceedings of the National Academy of Sciences of USA, 1979, 76, 3325–3329.
  • 11. Miura, K, Yamamoto, S, A scanning acoustic microscope discriminates cancer cells in fluid, Scientific Reports, 2015, 5, 15243.
  • 12. Strohm, EM, Kolios, MC. In IEEE Ultrasonics Symposium, Rome, Italy, 2009, pp 49-52.
  • 13. Strohm, EM, Czarnota, GJ, Kolios, MC, Quantitative Measurements of Apoptotic Cell Properties Using Acoustic Microscopy, IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control, 2010, 57, 2293-304.
  • 14. Saijo, Y, Sasaki, H, Sato, M, Nitta, S, Tanaka, M, Visualization of human umbilical vein endothelial cells by acoustic microscopy, Ultrasonics, 2000, 38, 396–399.
  • 15. Shelke, A, Brand, S, Kundu, T, Bereiter-Hahn, J, Blasé, C. In SPIE Conference on Health Monitoring of Structural and Biological Systems, San Diego, California, USA, 2012, pp 83481T.
  • 16. Soon, TTK, Chean, TW, Yamada, H, Takahashi, K, Hozumi, N, Kobayashi, K, Yoshida, S, Effects of anticancer drugs on glia-glioma brain tumor model characterized by acoustic impedance microscopy, Japanese Journal of Applied Physics, 2017, 56, 07JF15.
  • 17. Hozumi, N, Gunawan, AI, Kajima, S, Yoshida, S, Saijo, Y, Kobayashi, K, Yamamoto, S. In IEEE Ultrasonics Symposium, Prague, Czech Republic, 2012, pp 1212-1215.
  • 18. Shaw, G, Morse, S, Ararat, M, Graham, FL, Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells, The FASEB Journal, 2002, 16(8), 869-871.
  • 19. Xiaoning, Xi, Xin, Li, Miyasaka, C, Kropf, M, Tittmann, BR, High Frequency Scanning Acoustic Microscopy as Diagnostic Tool in Tissue Science, Journal of Biotechnology and Biomaterials, 2013, 3, 1000160.
  • 20. Azhari, H, Basics of Biomedical Ultrasound for Engineers; Wiley-IEEE Press: Hoboken, NJ, USA, 2010.
Birincil Dil en
Konular Mühendislik
Yayımlanma Tarihi 31-12-2018
Dergi Bölümü Makaleler
Yazarlar

Yazar: Bükem Bilen (Sorumlu Yazar)
Kurum: BOGAZICI UNIVERSITY
Ülke: Turkey


Yazar: Nural Pastaci Ozsobaci
Kurum: ISTANBUL UNIVERSITY
Ülke: Turkey


Yazar: Fatma Ates Alkan
Kurum: ISTANBUL UNIVERSITY
Ülke: Turkey


Yazar: Mehmet Burcin Unku
Kurum: BOGAZICI UNIVERSITY
Ülke: Turkey


Bibtex @araştırma makalesi { cbayarfbe454158, journal = {Celal Bayar Üniversitesi Fen Bilimleri Dergisi}, issn = {1305-130X}, eissn = {1305-1385}, address = {Celal Bayar Üniversitesi}, year = {2018}, volume = {14}, pages = {455 - 460}, doi = {10.18466/cbayarfbe.454158}, title = {Visualization of Human Kidney Embryonic Cells by Acoustic Microscopy}, key = {cite}, author = {Pastaci Ozsobaci, Nural and Unku, Mehmet Burcin and Ates Alkan, Fatma and Bilen, Bükem} }
APA Bilen, B , Pastaci Ozsobaci, N , Ates Alkan, F , Unku, M . (2018). Visualization of Human Kidney Embryonic Cells by Acoustic Microscopy. Celal Bayar Üniversitesi Fen Bilimleri Dergisi, 14 (4), 455-460. DOI: 10.18466/cbayarfbe.454158
MLA Bilen, B , Pastaci Ozsobaci, N , Ates Alkan, F , Unku, M . "Visualization of Human Kidney Embryonic Cells by Acoustic Microscopy". Celal Bayar Üniversitesi Fen Bilimleri Dergisi 14 (2018): 455-460 <http://dergipark.gov.tr/cbayarfbe/issue/41718/454158>
Chicago Bilen, B , Pastaci Ozsobaci, N , Ates Alkan, F , Unku, M . "Visualization of Human Kidney Embryonic Cells by Acoustic Microscopy". Celal Bayar Üniversitesi Fen Bilimleri Dergisi 14 (2018): 455-460
RIS TY - JOUR T1 - Visualization of Human Kidney Embryonic Cells by Acoustic Microscopy AU - Bükem Bilen , Nural Pastaci Ozsobaci , Fatma Ates Alkan , Mehmet Burcin Unku Y1 - 2018 PY - 2018 N1 - doi: 10.18466/cbayarfbe.454158 DO - 10.18466/cbayarfbe.454158 T2 - Celal Bayar Üniversitesi Fen Bilimleri Dergisi JF - Journal JO - JOR SP - 455 EP - 460 VL - 14 IS - 4 SN - 1305-130X-1305-1385 M3 - doi: 10.18466/cbayarfbe.454158 UR - http://dx.doi.org/10.18466/cbayarfbe.454158 Y2 - 2018 ER -
EndNote %0 Celal Bayar Üniversitesi Fen Bilimleri Dergisi Visualization of Human Kidney Embryonic Cells by Acoustic Microscopy %A Bükem Bilen , Nural Pastaci Ozsobaci , Fatma Ates Alkan , Mehmet Burcin Unku %T Visualization of Human Kidney Embryonic Cells by Acoustic Microscopy %D 2018 %J Celal Bayar Üniversitesi Fen Bilimleri Dergisi %P 1305-130X-1305-1385 %V 14 %N 4 %R doi: 10.18466/cbayarfbe.454158 %U 10.18466/cbayarfbe.454158
ISNAD Bilen, Bükem , Pastaci Ozsobaci, Nural , Ates Alkan, Fatma , Unku, Mehmet Burcin . "Visualization of Human Kidney Embryonic Cells by Acoustic Microscopy". Celal Bayar Üniversitesi Fen Bilimleri Dergisi 14 / 4 (Aralık 2018): 455-460. http://dx.doi.org/10.18466/cbayarfbe.454158