Yıl 2018, Cilt 21, Sayı 4, Sayfalar 387 - 395 2018-12-30

Histomorphometric Assessment of the Impact of Bovine Demineralized Bone Graft on Bone Healing Versus Autogenous, Allogeneic and Synthetic Grafts in Experimentally- Induced Critical Size Bone Defects in Rats
Ratlarda Deneysel Olarak Oluşturulan Kritik boyutlu kemik defektlerine uygulanan sığır kaynaklı deminarelize kemik greftininin kemik iyiyleşmesine olan etkisinin otojen,allojenik ve sentetik greftlerle karşılaştırılmasının histomorfometrik olarak incelenmesi

Turgay Peyami HOCAOĞLU [1] , Sadık GENÇOĞLAN [2] , Murat ARSLAN [3] , M. Emre BENLİDAYI [4] , Mehmet KÜRKÇÜ [5]

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Aim: Bone tissue has the ability to heal itself (regeneration) and may restore its morphology and function when injured. However, healing may be limited in the case of large wounds. A “critical-size defect” is an intraosseous wound in a particular bone and species of animal that will not heal spontaneously morphologically and functionally during the lifetime of the animal. Autogenous bone grafts have been regarded as “gold standard” for treatment of critical-size bone defects. Known drawbacks of autogenous bone graft have led to research efforts focusing on different graft materials and resulted in several alternative substitutes including xenografts, allografts and synthetic graft materials. The aim of the present study was to perform a histomorphometric study to investigate the effect of bovine demineralized bone graft on bone healing in comparison to autogenous, allogeneic and synthetic graft materials when applied into critical size bone defects with a diameter of 5 mm. Materials and methods: Experimental animals were divided into 4 groups, each having 8 rats. In the control group, a mandibular defect was created and then filled with a bovine graft (Integros Bone Plus XS Adana/Turkey). In the experimental groups, autogenous bone was reinserted into the critical-size defect which was created using a trephine bur in Group I (autogenous group) and Group II received a human graft (Korea Bone Bank (KBB) Gasandong Keumcheongu Seoul/South Korea) to fill the critical-size defect. For Group III, a synthetic bone graft β-tricalcium phosphate (Cerasorb North Carolina/USA) was applied on the critical-size bone defect. Specimens were obtained for histomorphometric examination and rats were sacrificed on day 28. Results: Histomorphometric examination performed on day 28 to evaluate the relative effects of different graft materials on new bone formation showed no significant difference in the volume of newly formed bone between groups receiving autogenous bone graft, allograft and bovine xenograft but a significant difference was observed versus synthetic bone graft group. Conclusion: While autogenous bone graft is currently regarded as the gold standard for bone regeneration, the difficulties in harvesting and application of autografts limit their use. Our results demonstrate that bovine bone graft may be used as a safe and effective alternative to autogenous bone graft.

Amaç: Kemik dokusu iyileşme özelliğine (rejenerasyon) sahiptir ve yaralanan kemik dokusu şekil ve fonksiyonunu yeniden kazanabilmektedir. Fakat yaralanmanın boyutu büyük olduğu zaman iyileşme sınırlı kalabilmektedir. Kritik boyutlu kemik defekti; kemik dokusunda, canlının yaşamı boyunca, şekil ve fonksiyon olarak, kendiliğinden tamamen iyileşmesinin mümkün olmayacağı boyuttaki defekt anlamına gelir. Kritik kemik defektlerinde tedavi için otojen greft uygulaması altın standart olarak kabul edilir. Otojen kemik greftinin bazı dezavantajları nedeniyle araştırmacılar çalışmalarını farklı greft materyalleri üzerinde yoğunlaştırmışlardır. Bu çalışmalar neticesinde Ksenogreft, Allogreft ve sentetik greft materyalleri gibi seçenekler ortaya çıkmıştır. Çalışmamızın amacı 5mm çapında kritik boyutlu kemik defektlerinde sığır kaynaklı demineralize kemik grefti uygulamasının kemik iyileşmesine etkisi ile aynı çaptaki defektlere otojenik, allojenik ve sentetik greft materyali uygulandığı zaman elde edilen iyileşmelerin histomorfometrik olarak incelenmesidir. Gereç ve Yöntemler: Deney hayvanları her grup 8 deney hayvanından oluşan 4 gruba ayrıldı. Kontrol grubunda mandibulada defekt oluşturulduktan sonra defekt sığır kaynaklı kemik grefti (Integros Bone Plus XS Adana/Türkiye) ile dolduruldu. Daha sonraki deney gruplarında; I. grupta oluşturulan kritik boyutlu defekte trefin frezle çıkartılan otojen kemik tekrar konuldu. II. grupta oluşturulan kritik boyutlu defekte insan kaynaklı kemik grefti (Korea Bone Bank (KBB) Gasandong Keumcheongu Seoul/Korea) uygulandı. III. grupta oluşturulan kritik boyutlu kemik defektine ise sentetik kemik grefti grubunda yer alan β-trikalsiyum fosfat (Cerasorb North Caroline/USA) uygulandı. 28 gün sonra ratlar öldürüldü.Her grup sakrifiye edilerek histomorfometrik incelemeye alındı. Sonuç: Farklı greft materyallerinin 28. günde yeni kemik oluşumuna olan etkisinin histomorfometrik olarak incelendiğinde otojen kemik grefti, allogreft ve sığır kaynaklı kemik grefti uygulanan gruplar arasında yeni oluşan kemik hacmi bakımından anlamlı bir fark bulunmazken, sentetik kemik grefti uygulanan grupla aralarındaki fark anlamlı bulunmuştur. Otojen kemik grefti günümüzde hala altın standart olarak kabul edilmesine rağmen, elde edilmesi ve uygulanmasındaki zorluklar nedeniyle çalışmamızda kullandığımız sığır kaynaklı kemik greftinin otojen kemik greftine alternatif olarak güvenilir ve etkili biçimde kullanılabileceği belirlenmiştir. 

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Orcid: 0000-0002-0281-8928
Yazar: Turgay Peyami HOCAOĞLU (Sorumlu Yazar)
Kurum: CUMHURİYET ÜNİVERSİTESİ DİŞ HEKİMLİĞİ FAKÜLTESİ AĞIZ DİŞ VE ÇENE CERRAHİSİ ABD.
Ülke: Turkey


Orcid: 0000-0001-6902-6653
Yazar: Sadık GENÇOĞLAN
Ülke: Turkey


Orcid: 0000-0003-1408-9168
Yazar: Murat ARSLAN
Kurum: CUMHURİYET ÜNİVERSİTESİ DİŞ HEKİMLİĞİ FAKÜLTESİ AĞIZ DİŞ VE ÇENE CERRAHİSİ ABD.
Ülke: Turkey


Yazar: M. Emre BENLİDAYI

Yazar: Mehmet KÜRKÇÜ

Bibtex @araştırma makalesi { cumudj475498, journal = {Cumhuriyet Dental Journal}, issn = {1302-5805}, eissn = {2146-2852}, address = {Cumhuriyet Üniversitesi}, year = {2018}, volume = {21}, pages = {387 - 395}, doi = {10.7126/cumudj.475498}, title = {Histomorphometric Assessment of the Impact of Bovine Demineralized Bone Graft on Bone Healing Versus Autogenous, Allogeneic and Synthetic Grafts in Experimentally- Induced Critical Size Bone Defects in Rats}, key = {cite}, author = {HOCAOĞLU, Turgay Peyami and GENÇOĞLAN, Sadık and ARSLAN, Murat and BENLİDAYI, M. Emre and KÜRKÇÜ, Mehmet} }
APA HOCAOĞLU, T , GENÇOĞLAN, S , ARSLAN, M , BENLİDAYI, M , KÜRKÇÜ, M . (2018). Histomorphometric Assessment of the Impact of Bovine Demineralized Bone Graft on Bone Healing Versus Autogenous, Allogeneic and Synthetic Grafts in Experimentally- Induced Critical Size Bone Defects in Rats. Cumhuriyet Dental Journal, 21 (4), 387-395. DOI: 10.7126/cumudj.475498
MLA HOCAOĞLU, T , GENÇOĞLAN, S , ARSLAN, M , BENLİDAYI, M , KÜRKÇÜ, M . "Histomorphometric Assessment of the Impact of Bovine Demineralized Bone Graft on Bone Healing Versus Autogenous, Allogeneic and Synthetic Grafts in Experimentally- Induced Critical Size Bone Defects in Rats". Cumhuriyet Dental Journal 21 (2018): 387-395 <http://dergipark.gov.tr/cumudj/issue/39823/475498>
Chicago HOCAOĞLU, T , GENÇOĞLAN, S , ARSLAN, M , BENLİDAYI, M , KÜRKÇÜ, M . "Histomorphometric Assessment of the Impact of Bovine Demineralized Bone Graft on Bone Healing Versus Autogenous, Allogeneic and Synthetic Grafts in Experimentally- Induced Critical Size Bone Defects in Rats". Cumhuriyet Dental Journal 21 (2018): 387-395
RIS TY - JOUR T1 - Histomorphometric Assessment of the Impact of Bovine Demineralized Bone Graft on Bone Healing Versus Autogenous, Allogeneic and Synthetic Grafts in Experimentally- Induced Critical Size Bone Defects in Rats AU - Turgay Peyami HOCAOĞLU , Sadık GENÇOĞLAN , Murat ARSLAN , M. Emre BENLİDAYI , Mehmet KÜRKÇÜ Y1 - 2018 PY - 2018 N1 - doi: 10.7126/cumudj.475498 DO - 10.7126/cumudj.475498 T2 - Cumhuriyet Dental Journal JF - Journal JO - JOR SP - 387 EP - 395 VL - 21 IS - 4 SN - 1302-5805-2146-2852 M3 - doi: 10.7126/cumudj.475498 UR - http://dx.doi.org/10.7126/cumudj.475498 Y2 - 2018 ER -
EndNote %0 Cumhuriyet Dental Journal Histomorphometric Assessment of the Impact of Bovine Demineralized Bone Graft on Bone Healing Versus Autogenous, Allogeneic and Synthetic Grafts in Experimentally- Induced Critical Size Bone Defects in Rats %A Turgay Peyami HOCAOĞLU , Sadık GENÇOĞLAN , Murat ARSLAN , M. Emre BENLİDAYI , Mehmet KÜRKÇÜ %T Histomorphometric Assessment of the Impact of Bovine Demineralized Bone Graft on Bone Healing Versus Autogenous, Allogeneic and Synthetic Grafts in Experimentally- Induced Critical Size Bone Defects in Rats %D 2018 %J Cumhuriyet Dental Journal %P 1302-5805-2146-2852 %V 21 %N 4 %R doi: 10.7126/cumudj.475498 %U 10.7126/cumudj.475498
ISNAD HOCAOĞLU, Turgay Peyami , GENÇOĞLAN, Sadık , ARSLAN, Murat , BENLİDAYI, M. Emre , KÜRKÇÜ, Mehmet . "Ratlarda Deneysel Olarak Oluşturulan Kritik boyutlu kemik defektlerine uygulanan sığır kaynaklı deminarelize kemik greftininin kemik iyiyleşmesine olan etkisinin otojen,allojenik ve sentetik greftlerle karşılaştırılmasının histomorfometrik olarak incelenmesi". Cumhuriyet Dental Journal 21 / 4 (Aralık 2018): 387-395. http://dx.doi.org/10.7126/cumudj.475498