Cilt 6, Sayı 10, Sayfalar 32 - 37

Nar suyu içerisindeki fenolik asitlerin HPLC-DAD ile belirlenmesi
Determination of phenolic acids in pomegranate juices by HPLC-DAD

Mürüvvet Karakaplan [1] , Mustafa Özcan [2]

126 188

Bu çalışmada taze sıkılmış nar suyu, ticari nar suyu ve nar suyu konsantresi içeceklerinde beş fenolik asit, gallik, klorojenik, kafeik, p-kumarik ve ferulik asit tespit edilmiştir. Bu amaçla, nar suyu seralarda fenolik asidin karakterizasyonu ve kantifikasyonu için, yüksek performanslı sıvı kromatografisi (HPLC) kullanan, fotodiyot dizi tespiti (DAD) kombinasyonlu analitik bir yöntem geliştirildi. Sonra nar suyu analizleri yapıldı. Tek tek bileşiklerin saptama limiti, gallik asit 0.1 mg / L, klorojenik asit 0.01 mg / L, kafeik asit 0.03 mg / L, p-kumarik asit 0.02 mg / L ve ferulik asit 0.04'tür (LOD, S / N = mg / L'dir. Bireysel bileşiklerin nicelik sınırı 0.4 mg / L gallik asit, klorojenik asit 0.05 mg / L, kafeik asit 0.1 mg / L, p-kumar asit 0.1 mg / L ve ferulik asit 0.1'dir (LOQ, S / N = 10) mg / L'dir. Ticari nar suyu içindeki bağıl standart sapma (% RSD), galli k asit% 0.08, klorojenik asit% 0.32, kafeik asit% 0.21, p-kumar asit% 0.08 ve ferulik asit% 0.38 olarak bulundu. Taze sıkılmış nar suyundaki göreli standart sapma (% RSD), gallik asit% 0.01, klorojenik asit% 0.46, kafeik asit% 0.17, p-kumarik asit% 0.04 ve ferulik asit% 0.8 olarak bulundu. Nar konsantresi suyundaki göreli standart sapma (% RSD), gallik asit% 0.78, klorojenik asit% 0.25, kafeik asit% 0.49, p-kumar asit% 0.36 ve ferulik asit% 0.17 olarak bulundu.

In this study, five phenolic acids, gallic, chlorogenic, caffeic, p-coumaric and ferulic acid were determined in freshly squeezed pomegranate juice, commercial pomegranate juice and pomegranate juice concentrate beverages.  For this purpose, an analytical method using high-performance liquid chromatography (HPLC), with a combination of photodiode array detection (DAD), was developed for the characterization and quantification of phenolic acid in pomegranate juices. Then the analyses of pomegranate juices was performed. The limit of detection (LOD, S/N=3) of individual compounds was gallic acid 0.1 mg/L, chlorogenic acid 0.01 mg/L, caffeic acid 0.03mg/L, p-coumaric acid 0.02 mg/L and ferulic acid 0.04 mg/L. The limit of quantitation (LOQ, S/N=10) of individual compounds was gallic acid 0.4 mg/L, chlorogenic acid 0.05 mg/L, caffeic acid 0.1 mg/L, p-coumaric acid 0.1 mg/L and ferulic acid 0.1 mg/L. The relative standard deviation (% RSD) in commericial pomegranate juice was found to be galli cacid % 0.08, chlorogenic acid % 0.32, caffeic acid % 0.21, p-coumaric acid % 0.08 and ferulic acid % 0.38. The relative standard deviation (% RSD) in freshly squeezed pomegranate juice was found to be gallic acid % 0.01, chlorogenic acid % 0.46, caffeic acid % 0.17, p-coumaric acid % 0.04 and ferulic acid % 0.8. The relative standard deviation (% RSD) in pomegranate concentrate juice was found to be gallic acid % 0.78, chlorogenic acid % 0.25, caffeic acid % 0.49, p-coumaric acid % 0.36 and ferulic acid % 0.17.

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Konular Mühendislik ve Temel Bilimler
Dergi Bölümü Makaleler
Yazarlar

Yazar: Mürüvvet Karakaplan
E-posta: muruvvetkarakaplan@hotmail.com
Kurum: İstanbul Teknik Üniversitesi
Ülke: Turkey


Yazar: Mustafa Özcan
E-posta: ozcanm@itu.edu.tr
Kurum: İstanbul Teknik Üniversitesi
Ülke: Turkey


Bibtex @araştırma makalesi { ejosat323971, journal = {Avrupa Bilim ve Teknoloji Dergisi}, issn = {}, address = {Şahin ORUÇ}, year = {}, volume = {6}, pages = {32 - 37}, doi = {}, title = {Nar suyu içerisindeki fenolik asitlerin HPLC-DAD ile belirlenmesi}, language = {tr}, key = {cite}, author = {Karakaplan, Mürüvvet and Özcan, Mustafa} } @araştırma makalesi { ejosat323971, journal = {Avrupa Bilim ve Teknoloji Dergisi}, issn = {}, address = {Şahin ORUÇ}, year = {}, volume = {6}, pages = {32 - 37}, doi = {}, title = {Determination of phenolic acids in pomegranate juices by HPLC-DAD}, language = {en}, key = {cite}, author = {Karakaplan, Mürüvvet and Özcan, Mustafa} }
APA Karakaplan, M , Özcan, M . (). Nar suyu içerisindeki fenolik asitlerin HPLC-DAD ile belirlenmesi. Avrupa Bilim ve Teknoloji Dergisi, 6 (10), 32-37. Retrieved from http://dergipark.gov.tr/ejosat/issue/30414/323971
MLA Karakaplan, M , Özcan, M . "Nar suyu içerisindeki fenolik asitlerin HPLC-DAD ile belirlenmesi". Avrupa Bilim ve Teknoloji Dergisi 6 (): 32-37 <http://dergipark.gov.tr/ejosat/issue/30414/323971>
Chicago Karakaplan, M , Özcan, M . "Nar suyu içerisindeki fenolik asitlerin HPLC-DAD ile belirlenmesi". Avrupa Bilim ve Teknoloji Dergisi 6 (): 32-37
RIS TY - JOUR T1 - Nar suyu içerisindeki fenolik asitlerin HPLC-DAD ile belirlenmesi AU - Mürüvvet Karakaplan , Mustafa Özcan Y1 - 2017 PY - 2017 N1 - DO - T2 - Avrupa Bilim ve Teknoloji Dergisi JF - Journal JO - JOR SP - 32 EP - 37 VL - 6 IS - 10 SN - -2148-2683 M3 - UR - Y2 - 2017 ER -
EndNote %0 Avrupa Bilim ve Teknoloji Dergisi Nar suyu içerisindeki fenolik asitlerin HPLC-DAD ile belirlenmesi %A Mürüvvet Karakaplan , Mustafa Özcan %T Nar suyu içerisindeki fenolik asitlerin HPLC-DAD ile belirlenmesi %D 2017 %J Avrupa Bilim ve Teknoloji Dergisi %P -2148-2683 %V 6 %N 10 %R %U