Yıl 2018, Cilt , Sayı 14, Sayfalar 348 - 352 2018-12-31

Lamiaceae Ailesine ait Bazı Bitki Türlerinin Genotoksik ve Antigenotoksik Etkileri
Genotoxic and Antigenotoxic Effects of Some Plant Species of Lamiaceae Family

Dudu Duygu Kilic [1] , Arif Ayar [2] , Ceren Baskan [3] , Tuba Yildirim [4]

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Lamiaceae ailesi, alternatif tıp tedavileri için ajan olarak kullanılan önemli bitki üyelerinden biridir. Çalışmada; Amasya’da bulunan Laminaceae familyasına ait üç farklı bitki türünün (Stachys annua, Scutellaria salviifolia, Nepeta nuda) antigenotoksik (SMART, Drosophila melanogaster somatik mutasyon ve rekombinasyon testi)  ve genotoksik (plazmid DNA etkileşim metodu) etkilerinin belirlenmesi amaçlanmıştır.

SMART analizleri, Etil metansülfonatın (EMS) genotoksik etkisinin, Scutellaria salviifolia bitki özütü ile ortadan kaldırıldığını ve antigenotoksik etkilerin bulunduğunu göstermiştir. Diğer bitki türlerinin farklı ekstrakt konsantrasyonları [(0.1, 0.25, 0.50 ve % 1 (v/v)], D. melanogaster üzerindeki lekelerin sayısını azaltmış ancak sonuçlar istatistiksel olarak anlamlı bulunmamıştır (p>0.05). SMART'a ek olarak, pUC18 plazmid DNA ile etkileşim de, N. nuda ve S. annua bitki özütlerinin (50 µg/mL-3.125 µg/mL) tüm konsantrasyonları kullanılarak analiz edilmiştir. Ekstraktlar Form I DNA'sında (supercoil) değişime neden olmamış, ancak Form II DNA'dan (açık dairesel) Form III DNA'ya aktarılmıştır. Ayrıca, S. salviifolia bitki ekstreleri DNA ve bant yoğunluğunu etkilememiştir. SMART sonuçları, S. salviifolia bitkilerinin yüksek etkili antijenotoksik etki gösterdiğini ancak genotoksik etkilerin olmadığını gösteren DNA etkileşimi analizleri ile desteklenmiştir.

Lamiaceae family is one of the members of important plants, using as a agents for alternative medicine treatments. Our aim was to investigate both antigenotoxic and genotoxic effects three different plants species belonging to Lamiaceae family: Stachys annua, Scutellaria salviifolia, Nepeta nuda in Amasya, using SMART (Drosophila melanogaster somatic mutation and recombination test) and also plasmid DNA interaction method, respectively. SMART analyses revealed that genotoxic effect of ethyl methanesulfonate (EMS) was eliminated by plant extract of S. salviifolia, indicating antigenotoxic effects.  Different extract concentrations [(0.1, 0.25, 0.50 and 1 % (v/v)] of other plants species decrased the flecks number on D. melanogaster but the results did not show any statistical significance (p>0.05). In addition to SMART, interaction with pUC18 plasmid DNA was also analysed by using all concentrations (50 µg/mL-3.125 µg/mL) of plant extracts N. nuda and S. annua. The extracts did not cause changed in Form I DNA (supercoil) but trasferred from Form II DNA (open circular) to Form III DNA. Moreover, there was no effect of S. salviifolia plant extracts on DNA and band density. SMART results were supported by DNA interaction analyses, cocncluding that S. salviifolia plants indicated the high-impact antigenotoxic effect, but not genotoxic effects.

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Orcid: 0000-0001-6425-6062
Yazar: Dudu Duygu Kilic
Kurum: AMASYA ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ
Ülke: Turkey


Orcid: 0000-0003-0473-4653
Yazar: Arif Ayar
Kurum: AMASYA ÜNİVERSİTESİ, SAĞLIK YÜKSEKOKULU

Orcid: 0000-0001-7849-4459
Yazar: Ceren Baskan
Kurum: AMASYA ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ

Orcid: 0000-0001-8575-4802
Yazar: Tuba Yildirim (Sorumlu Yazar)

Bibtex @araştırma makalesi { ejosat430874, journal = {Avrupa Bilim ve Teknoloji Dergisi}, issn = {}, eissn = {2148-2683}, address = {Osman Sağdıç}, year = {2018}, volume = {}, pages = {348 - 352}, doi = {10.31590/ejosat.430874}, title = {Genotoxic and Antigenotoxic Effects of Some Plant Species of Lamiaceae Family}, key = {cite}, author = {Kilic, Dudu Duygu and Ayar, Arif and Baskan, Ceren and Yildirim, Tuba} }
APA Kilic, D , Ayar, A , Baskan, C , Yildirim, T . (2018). Genotoxic and Antigenotoxic Effects of Some Plant Species of Lamiaceae Family. Avrupa Bilim ve Teknoloji Dergisi, (14), 348-352. DOI: 10.31590/ejosat.430874
MLA Kilic, D , Ayar, A , Baskan, C , Yildirim, T . "Genotoxic and Antigenotoxic Effects of Some Plant Species of Lamiaceae Family". Avrupa Bilim ve Teknoloji Dergisi (2018): 348-352 <http://dergipark.gov.tr/ejosat/issue/40225/430874>
Chicago Kilic, D , Ayar, A , Baskan, C , Yildirim, T . "Genotoxic and Antigenotoxic Effects of Some Plant Species of Lamiaceae Family". Avrupa Bilim ve Teknoloji Dergisi (2018): 348-352
RIS TY - JOUR T1 - Genotoxic and Antigenotoxic Effects of Some Plant Species of Lamiaceae Family AU - Dudu Duygu Kilic , Arif Ayar , Ceren Baskan , Tuba Yildirim Y1 - 2018 PY - 2018 N1 - doi: 10.31590/ejosat.430874 DO - 10.31590/ejosat.430874 T2 - Avrupa Bilim ve Teknoloji Dergisi JF - Journal JO - JOR SP - 348 EP - 352 VL - IS - 14 SN - -2148-2683 M3 - doi: 10.31590/ejosat.430874 UR - http://dx.doi.org/10.31590/ejosat.430874 Y2 - 2018 ER -
EndNote %0 Avrupa Bilim ve Teknoloji Dergisi Genotoxic and Antigenotoxic Effects of Some Plant Species of Lamiaceae Family %A Dudu Duygu Kilic , Arif Ayar , Ceren Baskan , Tuba Yildirim %T Genotoxic and Antigenotoxic Effects of Some Plant Species of Lamiaceae Family %D 2018 %J Avrupa Bilim ve Teknoloji Dergisi %P -2148-2683 %V %N 14 %R doi: 10.31590/ejosat.430874 %U 10.31590/ejosat.430874
ISNAD Kilic, Dudu Duygu , Ayar, Arif , Baskan, Ceren , Yildirim, Tuba . "Lamiaceae Ailesine ait Bazı Bitki Türlerinin Genotoksik ve Antigenotoksik Etkileri". Avrupa Bilim ve Teknoloji Dergisi / 14 (Aralık 2019): 348-352. http://dx.doi.org/10.31590/ejosat.430874