Yıl 2019, Cilt 8, Sayı 1, Sayfalar 40 - 49 2019-01-01

Light and Scanning-Electron Microscopic Study of Eisenia fetida Coelomocytes after Copper Oxychloride Exposure

VOLKAN KILIÇ [1] , GOZDE AYDOGAN KILIC [2]

5 20

Morphological alterations in Eisenia fetida coelomocytes are recommended biomarkers for soil contamination surveys. Copper oxychloride is a widely used agrochemical agent that causes excessive amounts of Cu accumulation in soils. The present study is a light and electron microscopic investigation of the morphological alterations in coelomocytes of E. fetida exposed to copper oxychloride. Exposure concentration was 350 mg/kg of artificial soil and exposure duration was 7 or 14 days. Colelomocytes were extrused into an ethanol and guaicol glyceryl (GGE) containing modified Hanks’ balanced solution (M-HBSS-GGE) by a simple low vacuum holding device and processed for light and scanning electron microscopy. Lipid peroxidation (LP) in the earthworms was also measured to evaluate oxidative stress (OS). Swelling and vesicular deformation were the early signs of toxicity in eleocytes after 7 days. After 14 days, complete loss of vesicles and spongy surface were observed in eleocytes. Granulocytes showed more dramatical changes. Nucleus fragmentation and membrane rupture were observed after 14 days. Increased LP in the earthworms was accompanying these changes.
Copper oxychloride, E. fetida, coelomocytes
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Yazarlar

Yazar: VOLKAN KILIÇ

Yazar: GOZDE AYDOGAN KILIC

Bibtex @ { estubtdc525535, journal = {Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji}, issn = {2667-4203}, address = {Eskişehir Teknik Üniversitesi}, year = {2019}, volume = {8}, pages = {40 - 49}, doi = {}, title = {Light and Scanning-Electron Microscopic Study of Eisenia fetida Coelomocytes after Copper Oxychloride Exposure}, key = {cite}, author = {KILIÇ, VOLKAN and KILIC, GOZDE AYDOGAN} }
APA KILIÇ, V , KILIC, G . (2019). Light and Scanning-Electron Microscopic Study of Eisenia fetida Coelomocytes after Copper Oxychloride Exposure. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, 8 (1), 40-49. Retrieved from http://dergipark.gov.tr/estubtdc/issue/43264/525535
MLA KILIÇ, V , KILIC, G . "Light and Scanning-Electron Microscopic Study of Eisenia fetida Coelomocytes after Copper Oxychloride Exposure". Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 8 (2019): 40-49 <http://dergipark.gov.tr/estubtdc/issue/43264/525535>
Chicago KILIÇ, V , KILIC, G . "Light and Scanning-Electron Microscopic Study of Eisenia fetida Coelomocytes after Copper Oxychloride Exposure". Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 8 (2019): 40-49
RIS TY - JOUR T1 - Light and Scanning-Electron Microscopic Study of Eisenia fetida Coelomocytes after Copper Oxychloride Exposure AU - VOLKAN KILIÇ , GOZDE AYDOGAN KILIC Y1 - 2019 PY - 2019 N1 - DO - T2 - Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji JF - Journal JO - JOR SP - 40 EP - 49 VL - 8 IS - 1 SN - 2667-4203- M3 - UR - Y2 - 2019 ER -
EndNote %0 Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji Light and Scanning-Electron Microscopic Study of Eisenia fetida Coelomocytes after Copper Oxychloride Exposure %A VOLKAN KILIÇ , GOZDE AYDOGAN KILIC %T Light and Scanning-Electron Microscopic Study of Eisenia fetida Coelomocytes after Copper Oxychloride Exposure %D 2019 %J Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji %P 2667-4203- %V 8 %N 1 %R %U
ISNAD KILIÇ, VOLKAN , KILIC, GOZDE AYDOGAN . "Light and Scanning-Electron Microscopic Study of Eisenia fetida Coelomocytes after Copper Oxychloride Exposure". Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 8 / 1 (Ocak 2019): 40-49.