Year 2019, Volume 58, Issue 1, Pages 32 - 38 2019-03-14

Investigation of the effects of zoledronic acid in breast cancer stem cell line (mcf7) for autophagic gene level
Zoledronik asit uygulamasının meme kanseri hücre hattı (mcf 7) kanser kök hücrelerinin üzerine otofaji gen seviyesindeki etkilerinin araştırılması

Fatih Oltulu [1] , Ayşegül Uysal [2] , Hadi Rouhrazi [3] , Kubilay Doğan Kılıç [4] , Duygu Çalık Kocatürk [5] , Gülperi Öktem [6]

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Aim: In this study we examined differences in the expression of genes associated with autophagy and CSCs for the treatment ZA applied via taking breast cancer stem cells as models.

Materials and Methods: CSCs with CD44high/CD24high surface markers have been isolated from the MCF-7 breast cancer cell line with flow cytometry methods. cDNA was synthesized for ZA treatment applied and not applied CSC group with total RNA isolation. Then, 41 essential autophagy-related gene expression levels were analyzed by qRT-PCR array analysis.

Results: When ZA treatment administrated group compared with non-treatment group, downregulation of TGM2, TNF, HSPA8 and upregulation of HSP90AA1 was detected.

Conclusion: There are differences of expression profiles of genes related to autophagy between ZA treated and non-ZA tread group. These findings may provide important information towards us to target CSCs to develop more effective treatments.

Amaç: Zoledronik asit (ZA) uygulanmış meme kanser kök hücrelerinde (KKH), kanser kök hücresi ve otofaji ile ilişkili gen ekspresyonlarındaki farklılıkları incelemek.

Gereç ve Yöntem: CD44yüksek/CD24yüksek yüzey belirteçleri pozitif olan MCF-7 meme kanseri hücre hattından KKH’ler akış sitometri metodu ile izole edilmiştir. ZA tedavisi uygulanmış ve uygulanmamış olan KKH gruplarının toplam RNA izolasyonu için cDNA sentezlenmiştir. Son olarak 41 adet otofaji için başlıca ilişkili olabilecek genlerin ekspresyon seviyeleri qRT-PCR analizi ile incelenmiştir.

Bulgular: ZA tedavisi uygulanan grup tedavi uygulanmayan grup ile karşılaştırıldığında TGM2, TNF, HSPA8 genlerinin azalmış regüle olduğu ve HSP90AA1 geninin artmış regüle olduğu görülmüştür.

Sonuç: Otofaji ile ilişkili genlerin ekspresyon profillerinde ZA tedavisi uygulanan ve uygulanmayan gruplar arasında anlamlı farklar bulunmuştur. Bu bulgular, KKH’lerine karşı hedef olabilecek daha etkili tedavi ajanları geliştirilebilmesi için önemli katkılar sağlayabilir.

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Primary Language tr
Subjects Health Care Sciences and Services
Journal Section Research Articles
Authors

Orcid: 0000-0001-6475-642X
Author: Fatih Oltulu (Primary Author)
Institution: Ege Üniversitesi Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, İzmir
Country: Turkey


Orcid: 0000-0002-9919-2254
Author: Ayşegül Uysal
Institution: Ege Üniversitesi Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, İzmir
Country: Turkey


Orcid: 0000-0002-1367-064X
Author: Hadi Rouhrazi
Institution: Ege Üniversitesi Sağlık Bilimleri Enstitüsü, Tıbbi Biyokimya Anabilim Dalı, İzmir
Country: Turkey


Orcid: 0000-0002-9484-0777
Author: Kubilay Doğan Kılıç
Institution: Ege Üniversitesi Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, İzmir
Country: Turkey


Orcid: 0000-0001-5995-8967
Author: Duygu Çalık Kocatürk
Institution: Ege Üniversitesi Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, İzmir
Country: Turkey


Orcid: 0000-0001-6227-9519
Author: Gülperi Öktem
Institution: Ege Üniversitesi Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, İzmir

Bibtex @research article { etd417985, journal = {Ege Tıp Dergisi}, issn = {1016-9113}, eissn = {2147-6500}, address = {Ege University}, year = {2019}, volume = {58}, pages = {32 - 38}, doi = {10.19161/etd.417985}, title = {Zoledronik asit uygulamasının meme kanseri hücre hattı (mcf 7) kanser kök hücrelerinin üzerine otofaji gen seviyesindeki etkilerinin araştırılması}, key = {cite}, author = {Oltulu, Fatih and Uysal, Ayşegül and Rouhrazi, Hadi and Kılıç, Kubilay Doğan and Çalık Kocatürk, Duygu and Öktem, Gülperi} }
APA Oltulu, F , Uysal, A , Rouhrazi, H , Kılıç, K , Çalık Kocatürk, D , Öktem, G . (2019). Zoledronik asit uygulamasının meme kanseri hücre hattı (mcf 7) kanser kök hücrelerinin üzerine otofaji gen seviyesindeki etkilerinin araştırılması. Ege Tıp Dergisi, 58 (1), 32-38. DOI: 10.19161/etd.417985
MLA Oltulu, F , Uysal, A , Rouhrazi, H , Kılıç, K , Çalık Kocatürk, D , Öktem, G . "Zoledronik asit uygulamasının meme kanseri hücre hattı (mcf 7) kanser kök hücrelerinin üzerine otofaji gen seviyesindeki etkilerinin araştırılması". Ege Tıp Dergisi 58 (2019): 32-38 <http://dergipark.gov.tr/etd/issue/43429/417985>
Chicago Oltulu, F , Uysal, A , Rouhrazi, H , Kılıç, K , Çalık Kocatürk, D , Öktem, G . "Zoledronik asit uygulamasının meme kanseri hücre hattı (mcf 7) kanser kök hücrelerinin üzerine otofaji gen seviyesindeki etkilerinin araştırılması". Ege Tıp Dergisi 58 (2019): 32-38
RIS TY - JOUR T1 - Zoledronik asit uygulamasının meme kanseri hücre hattı (mcf 7) kanser kök hücrelerinin üzerine otofaji gen seviyesindeki etkilerinin araştırılması AU - Fatih Oltulu , Ayşegül Uysal , Hadi Rouhrazi , Kubilay Doğan Kılıç , Duygu Çalık Kocatürk , Gülperi Öktem Y1 - 2019 PY - 2019 N1 - doi: 10.19161/etd.417985 DO - 10.19161/etd.417985 T2 - Ege Tıp Dergisi JF - Journal JO - JOR SP - 32 EP - 38 VL - 58 IS - 1 SN - 1016-9113-2147-6500 M3 - doi: 10.19161/etd.417985 UR - https://doi.org/10.19161/etd.417985 Y2 - 2018 ER -
EndNote %0 Ege Journal of Medicine Zoledronik asit uygulamasının meme kanseri hücre hattı (mcf 7) kanser kök hücrelerinin üzerine otofaji gen seviyesindeki etkilerinin araştırılması %A Fatih Oltulu , Ayşegül Uysal , Hadi Rouhrazi , Kubilay Doğan Kılıç , Duygu Çalık Kocatürk , Gülperi Öktem %T Zoledronik asit uygulamasının meme kanseri hücre hattı (mcf 7) kanser kök hücrelerinin üzerine otofaji gen seviyesindeki etkilerinin araştırılması %D 2019 %J Ege Tıp Dergisi %P 1016-9113-2147-6500 %V 58 %N 1 %R doi: 10.19161/etd.417985 %U 10.19161/etd.417985
ISNAD Oltulu, Fatih , Uysal, Ayşegül , Rouhrazi, Hadi , Kılıç, Kubilay Doğan , Çalık Kocatürk, Duygu , Öktem, Gülperi . "Zoledronik asit uygulamasının meme kanseri hücre hattı (mcf 7) kanser kök hücrelerinin üzerine otofaji gen seviyesindeki etkilerinin araştırılması". Ege Tıp Dergisi 58 / 1 (March 2019): 32-38. https://doi.org/10.19161/etd.417985