Yıl 2019, Cilt 5, Sayı 1, Sayfalar 148 - 158 2019-01-04

Comparison of effects of melatonin, pentoxifylline and dimethyl sulfoxide in experimental liver ischemia-reperfusion injury by three different methods

Zeliha Türkyılmaz [1] , Ahmet Hatipoğlu [2] , Mahmut Yüksel [3] , Nurettin Aydoğdu [4] , Gülara Hüseyinova [5]

20 75

Objectives: Liver transplantation is increasingly being used in the treatment of end-stage liver disease. Ischemia-reperfusion injury is one of the major problems encountered in transplantation. In this study, we aimed to compare the effects of melatonin, pentoxifylline, and dimethyl sulfoxide (DMSO), in hepatic ischemia-reperfusion injury with different methods such as biochemical/ultrastructural changes and hepatobiliary scintigraphy.

Methods: Thirty rabbits were used in the Laboratory of Experimental Animals of Trakya University under appropriate conditions. Sham laparotomy and only ischemia reperfusion group were planned. They were used melatonin, pentoxifylline, and DMSO after ischemia-reperfusion in the other three groups. 6 rabbits were randomly selected for each group. Rabbits in all groups were subjected to liver scintigraphy. Following scintigraphy, 2 cm2 of liver tissue was removed to examining for liver antioxidant enzyme levels (superoxide dismutase [SOD] and glutathione peroxidase [GPx]) and for liver electron microscopy.

Results: Pentoxifylline and melatonin protected significantly uptake and excretion functions in liver scintigraphy. When the effects of all three substances were examined by electron microscopy, it was found that the three substances protected the liver from the effects of ischemia-reperfusion damage at varying rates. All three agents were found to protect SOD and GPx from falling in various amounts.

Conclusions: Studies to prevent ischemia-reperfusion injury, which may develop as a result of the Pringle maneuver applied to liver transplantations as well as to liver resections or liver injuries, still maintain their popularity. In our study, the effects of agents were identified in three different ways. Ischemia-reperfusion injury-reducing effect of pentoxifylline gave parallel results with three methods. 

Pentoxifylline, melatonin, dimethyl sulfoxide, ischemia, reperfusion
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Orcid: 0000-0002-0012-2089
Yazar: Zeliha Türkyılmaz (Sorumlu Yazar)
Kurum: Department of General Surgery, Edirne 1. Murat State Hospital, Edirne, Turkey
Ülke: Turkey


Orcid: 0000-0003-2691-2529
Yazar: Ahmet Hatipoğlu
Kurum: Department of General Surgery, Trakya University School of Medicine, Edirne, Turkey
Ülke: Turkey


Orcid: 0000-0003-0159-6873
Yazar: Mahmut Yüksel
Kurum: Department of Nucleer Medicine, Altınbaş University Medical Park Bahçelievler Hostipal, İstanbul, Turkey
Ülke: Turkey


Orcid: 0000-0002-6341-5137
Yazar: Nurettin Aydoğdu
Kurum: Department of Physiology, Trakya University School of Medicine, Edirne, Turkey
Ülke: Turkey


Orcid: 0000-0001-7941-2080
Yazar: Gülara Hüseyinova
Kurum: Department of Pathology, Trakya University School of Medicine, Edirne, Turkey
Ülke: Turkey


Bibtex @araştırma makalesi { eurj421296, journal = {The European Research Journal}, issn = {}, eissn = {2149-3189}, address = {Sağlık Araştırmaları ve Stratejileri Derneği}, year = {2019}, volume = {5}, pages = {148 - 158}, doi = {10.18621/eurj.421296}, title = {Comparison of effects of melatonin, pentoxifylline and dimethyl sulfoxide in experimental liver ischemia-reperfusion injury by three different methods}, key = {cite}, author = {Türkyılmaz, Zeliha and Aydoğdu, Nurettin and Yüksel, Mahmut and Hüseyinova, Gülara and Hatipoğlu, Ahmet} }
APA Türkyılmaz, Z , Hatipoğlu, A , Yüksel, M , Aydoğdu, N , Hüseyinova, G . (2019). Comparison of effects of melatonin, pentoxifylline and dimethyl sulfoxide in experimental liver ischemia-reperfusion injury by three different methods. The European Research Journal, 5 (1), 148-158. DOI: 10.18621/eurj.421296
MLA Türkyılmaz, Z , Hatipoğlu, A , Yüksel, M , Aydoğdu, N , Hüseyinova, G . "Comparison of effects of melatonin, pentoxifylline and dimethyl sulfoxide in experimental liver ischemia-reperfusion injury by three different methods". The European Research Journal 5 (2019): 148-158 <http://dergipark.gov.tr/eurj/issue/40357/421296>
Chicago Türkyılmaz, Z , Hatipoğlu, A , Yüksel, M , Aydoğdu, N , Hüseyinova, G . "Comparison of effects of melatonin, pentoxifylline and dimethyl sulfoxide in experimental liver ischemia-reperfusion injury by three different methods". The European Research Journal 5 (2019): 148-158
RIS TY - JOUR T1 - Comparison of effects of melatonin, pentoxifylline and dimethyl sulfoxide in experimental liver ischemia-reperfusion injury by three different methods AU - Zeliha Türkyılmaz , Ahmet Hatipoğlu , Mahmut Yüksel , Nurettin Aydoğdu , Gülara Hüseyinova Y1 - 2019 PY - 2019 N1 - doi: 10.18621/eurj.421296 DO - 10.18621/eurj.421296 T2 - The European Research Journal JF - Journal JO - JOR SP - 148 EP - 158 VL - 5 IS - 1 SN - -2149-3189 M3 - doi: 10.18621/eurj.421296 UR - http://dx.doi.org/10.18621/eurj.421296 Y2 - 2018 ER -
EndNote %0 The European Research Journal Comparison of effects of melatonin, pentoxifylline and dimethyl sulfoxide in experimental liver ischemia-reperfusion injury by three different methods %A Zeliha Türkyılmaz , Ahmet Hatipoğlu , Mahmut Yüksel , Nurettin Aydoğdu , Gülara Hüseyinova %T Comparison of effects of melatonin, pentoxifylline and dimethyl sulfoxide in experimental liver ischemia-reperfusion injury by three different methods %D 2019 %J The European Research Journal %P -2149-3189 %V 5 %N 1 %R doi: 10.18621/eurj.421296 %U 10.18621/eurj.421296
ISNAD Türkyılmaz, Zeliha , Hatipoğlu, Ahmet , Yüksel, Mahmut , Aydoğdu, Nurettin , Hüseyinova, Gülara . "Comparison of effects of melatonin, pentoxifylline and dimethyl sulfoxide in experimental liver ischemia-reperfusion injury by three different methods". The European Research Journal 5 / 1 (Ocak 2019): 148-158. http://dx.doi.org/10.18621/eurj.421296