Year 2018, Volume 43, Issue 6, Pages 917 - 924 2018-11-01

TURUNÇGİL LİFİNDE FLAVONOİDLERİN BELİRLENMESİ VE ÜRETİM METODUNUN TOPLAM FENOLİK MİKTARI, TOPLAM FLAVONOİD MİKTARI, TOPLAM KAROTENOİT MİKTARI VE OKSİJEN RADİKAL SOĞURMA KAPASİTESİNE ETKİSİ
DETERMINATION OF FLAVONOIDS IN CITRUS FIBER AND THE IMPACT OF PROCESSING METHOD ON TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT, TOTAL CAROTENOID CONTENT AND OXYGEN RADICAL ABSORBANCE CAPACITY

Ayça Gedikoğlu [1] , Andrew Douglas Clarke [2] , Ingolf Gruen [3]

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Bu çalışmanın amacı turunçgil kabuğundan elde edilen liflerin ters fazlı yüksek basınçlı sıvı kromatografisi yöntemi ile flavonoidlerinin belirlenmesidir. Ayrıca, bu çalışmada turunçgil kabuklarından lif hazırlanırken kullanılan normal veya sıcak yıkama işlemlerinin toplam fenol miktarı, toplam flavonoid miktarı, toplam karotenoid miktarı ve oksijen radikal soğurma kapasitesi değerine etkisi araştırılmaktadır. Kullanılan HPLC metodu çoklu metoksiflavon ve flavanollerin ayrılmasını sağlayan ve hızlı sonuç veren bir yöntemdir. Kuersetin, kemferol, sinensetin, nobiletin, heptamethoksiflavon ve tangeretin belirlenmiştir.  Nobiletin 3.33 mg/g  kuru ağırlık ile en yüksek miktarda bulunan flavonoiddir. Sinensetin 1.96 mg/g kuru ağırlık  ile, heptametoksiflavon ise 1.24 mg/g kuru ağırlık ile takip etmektedir. Sıcak yıkama veya normal yıkamanın toplam flavonoid içeriği ve toplam karotenoid içeriğine etkisi görülmemiştir (P >0.05).  Ancak, toplam fenolik madde ve oksijen radikal soğurma kapasitesi değeri sıcak yıkama yapılan liflerde daha düşük bulunmuştur (P < 0.05). 

The objective of this study is to evaluate the presence of flavonoids in citrus fıber using reverse-phase high-pressure liquid chromatography (RP-HPLC). In this study, we also investigate the effect of regular and hot-washing preparations on total polyphenol content (TPC), total flavonoid content (TFC), oxygen radical absorbance capacity (ORAC) and total carotenoid content (TCC) of citrus fiber. The HPLC method used provided fast results and good separation for polymethoxyflavones and flavonols. Quercetin and kaempferol (flavonols), and sinensetin, nobiletin, heptamethoxyflavone, and tangeretin (polymethoxyflavones) were identified. Nobiletin had the highest concentration with 3.33 mg/g dry weight followed by sinensetin (1.96 mg/g dry weight) and heptamethoxyflavone (1.24 mg/g dry weight), respectively. The TFC and TCC of citrus fiber were unaffected (P >0.05) by the citrus fiber production procedure; however, the TPC and the ORAC values of citrus fiber prepared using hot-washing were significantly lower (P < 0.05). 

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Primary Language en
Subjects Science
Published Date 2018
Journal Section Articles
Authors

Author: Ayça Gedikoğlu (Primary Author)
Institution: KONYA GIDA VE TARIM ÜNİVERSİTESİ
Country: Turkey


Author: Andrew Douglas Clarke
Institution: University of Missouri
Country: United States


Author: Ingolf Gruen
Institution: University of Missouri
Country: United States


Bibtex @research article { gida442798, journal = {GIDA}, issn = {1300-3070}, eissn = {1309-6273}, address = {Gıda Teknolojisi Derneği}, year = {2018}, volume = {43}, pages = {917 - 924}, doi = {10.15237/gida.GD18072}, title = {DETERMINATION OF FLAVONOIDS IN CITRUS FIBER AND THE IMPACT OF PROCESSING METHOD ON TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT, TOTAL CAROTENOID CONTENT AND OXYGEN RADICAL ABSORBANCE CAPACITY}, key = {cite}, author = {Gedikoğlu, Ayça and Clarke, Andrew Douglas and Gruen, Ingolf} }
APA Gedikoğlu, A , Clarke, A , Gruen, I . (2018). DETERMINATION OF FLAVONOIDS IN CITRUS FIBER AND THE IMPACT OF PROCESSING METHOD ON TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT, TOTAL CAROTENOID CONTENT AND OXYGEN RADICAL ABSORBANCE CAPACITY. GIDA, 43 (6), 917-924. DOI: 10.15237/gida.GD18072
MLA Gedikoğlu, A , Clarke, A , Gruen, I . "DETERMINATION OF FLAVONOIDS IN CITRUS FIBER AND THE IMPACT OF PROCESSING METHOD ON TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT, TOTAL CAROTENOID CONTENT AND OXYGEN RADICAL ABSORBANCE CAPACITY". GIDA 43 (2018): 917-924 <http://dergipark.gov.tr/gida/issue/39712/442798>
Chicago Gedikoğlu, A , Clarke, A , Gruen, I . "DETERMINATION OF FLAVONOIDS IN CITRUS FIBER AND THE IMPACT OF PROCESSING METHOD ON TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT, TOTAL CAROTENOID CONTENT AND OXYGEN RADICAL ABSORBANCE CAPACITY". GIDA 43 (2018): 917-924
RIS TY - JOUR T1 - DETERMINATION OF FLAVONOIDS IN CITRUS FIBER AND THE IMPACT OF PROCESSING METHOD ON TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT, TOTAL CAROTENOID CONTENT AND OXYGEN RADICAL ABSORBANCE CAPACITY AU - Ayça Gedikoğlu , Andrew Douglas Clarke , Ingolf Gruen Y1 - 2018 PY - 2018 N1 - doi: 10.15237/gida.GD18072 DO - 10.15237/gida.GD18072 T2 - GIDA JF - Journal JO - JOR SP - 917 EP - 924 VL - 43 IS - 6 SN - 1300-3070-1309-6273 M3 - doi: 10.15237/gida.GD18072 UR - https://doi.org/10.15237/gida.GD18072 Y2 - 2018 ER -
EndNote %0 THE JOURNAL OF FOOD DETERMINATION OF FLAVONOIDS IN CITRUS FIBER AND THE IMPACT OF PROCESSING METHOD ON TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT, TOTAL CAROTENOID CONTENT AND OXYGEN RADICAL ABSORBANCE CAPACITY %A Ayça Gedikoğlu , Andrew Douglas Clarke , Ingolf Gruen %T DETERMINATION OF FLAVONOIDS IN CITRUS FIBER AND THE IMPACT OF PROCESSING METHOD ON TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT, TOTAL CAROTENOID CONTENT AND OXYGEN RADICAL ABSORBANCE CAPACITY %D 2018 %J GIDA %P 1300-3070-1309-6273 %V 43 %N 6 %R doi: 10.15237/gida.GD18072 %U 10.15237/gida.GD18072
ISNAD Gedikoğlu, Ayça , Clarke, Andrew Douglas , Gruen, Ingolf . "DETERMINATION OF FLAVONOIDS IN CITRUS FIBER AND THE IMPACT OF PROCESSING METHOD ON TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT, TOTAL CAROTENOID CONTENT AND OXYGEN RADICAL ABSORBANCE CAPACITY". GIDA 43 / 6 (November 2018): 917-924. https://doi.org/10.15237/gida.GD18072