Yıl 2018, Cilt 31, Sayı 4, Sayfalar 1033 - 1046 2018-12-01

Microorganism and Pretreatment Effect on Lignocellulosic Bioethanol Production

Asiye Gül BAYRAKCI OZDINGIS [1] , Gunnur KOCAR [2]

24 29

The effects of pretreatments applied to raw materials and microorganism selection in lignocellulosic bioethanol production were investigated. It has been found that the yield of enzymatic pretreatment process applied after the chemical pretreatment is about 4 times higher than that only chemical. Enzymatic pretreatment used process yield is 3.5 times higher than that chemical pretreatment. When the microorganism ethanol production yield values of Saccharomyces cerevisiae and Pichia stipitis were examined, it was found that S.cerevisiae was superior to P.stipitis in chemical pretreated reactors (about 1.7 times higher) while P. stipitis yield was higher about 1.2 times in enzymatic pretreated reactors. When the reactors which have been pretreated with both chemical and enzymatic hydrolysis and P. stipitis and S. cerevisiae used separately were examined, it was observed that there was not a great difference in terms of ethanol production yield. C. thermocellum’s ethanol yield was found about 3 times lower than the S. cerevisiae and P. stipitis. According to the obtained data, it was seen that S. cerevisiae could produce ethanol with higher efficiency than P. stipitis. At the same time, the difficulty of C. thermocellum’s production conditions, high energy demand and high risk of contamination, and low ethanol production yield, it is thought that it can only be used in the research phase for now. But in particular, by investigating extracellular cellulase enzyme system of C. thermocellum, genetic modifications are predicted to play an important role in the future in the second generation bioethanol production process.
Clostridum thermocellum, Saccharomyces cerevisiae, Lignocellulosic ethanol, Pichia stipitis, Pretreatment
  • Bayrakcı Özdingiş, A. G., “Second generation bioethanol production by using water hyacinth and other lignocellulosic residues,” Phd Thesis, Ege University Institute of Solar Energy, Izmir, Turkey, (2017).
  • Saddler J. N. and Chan, M. K. H., “Optimization of Clostridium thermocellum growth on cellulose and pretreated wood substrates,” Eur J Appl Microbiol, 16(2-3):99-104pp., (1982).
  • Kasavi, C., “A system based rational approach to improve first and second generation bioethanol production by Saccharomyces cerevisiae,” PhD Thesis, Boğaziçi University Chemical Engineering, İstanbul, Turkey, (2013).
  • Arora, R., Behera, S., and Kumar, S., “Bioprospecting thermophilic/thermotolerant microbes for production of lignocellulosic ethanol: A future perspective,” Renew Sust Energ Rev, 51:699-717pp. (2015).
  • Goncalves, F. A., Ruiz, H. A., dos Santos, E. S., Teixeira, J. A., de Macedo, G. R., “Bioethanol production by Saccharomyces cerevisiae, Pichia stipitis and Zymomonas mobilis from delignified coconut fibre mature and lignin extraction according to biorefinery concept,” Renew Energ, 94:353-365pp., (2016).
  • Sills, D. and Gossett, J. M., “Assessment of commercial hemicellulases for saccharification of alkaline pretreated perennial biomass,” Bioresource Technol, 102:1389-1398pp., (2011).
  • Xu, C., Qin, Y., Li, Y., Ji, Y., Huang, J., Song, H., and Xu, J., “Factors influencing cellulosome activity in consolidated bioprocessing of cellulosic ethanol,” Bioresource Technol, 101(24):9560–9569pp., (2010).
  • Li, J., Zhou, P., Liu, H., Wu, K., Kang, X., Gong, Y., Xiao, W., Lin, J., and Liu, Z., “A comparison of fermentation strategies for cellulosic ethanol production from NaOH-soaked sugarcane bagasse at high solid loading with decreased cellulase loading,” Ind Crop Prod, 62:446–452pp., (2014).
  • Richard, P., Verho, R., Putkonen, M., Londesborough, J., and Penttilä, M, “Production of ethanol from L-arabinose by Saccharomyces cerevisiae containing a fungal L-arabinose pathway”, FEMS Yeast Res, 3(2):185-189pp., (2003).
  • Becker, J. and Boles, E., “A modified Saccharomyces cerevisiae strain that consumes l-arabinose and produces ethanol,” Appl Environ Microb, 69(7):4144-4150pp., (2003).
  • Brown, S. D., Guss, A. M., Karpinets, T. V., Parks, J. M., Smolin, N., Yang, S., Land, M. L., Klingeman, D. M., Bhandiwad, A., Rodriguez, M., et al., “Mutant alcohol dehydrogenase leads to improved ethanol tolerance in Clostridium thermocellum,” P Natl Acad Sci-Biol, 108(33):13752-13757pp., (2011).
  • Yang, S., Giannone, R. J., Dice, L., Yang, Z. K., Engle, N. G., Tschaplinski, T. J., Hettich, R. L., and Brown, S. D., “Clostridium thermocellum ATCC27405 transcriptomic, metabolomic and proteomic profiles after ethanol stress,” BMC Genomics, 13:336p., (2012).
  • Zhu, X., Cui, J., Feng, Y., Fa, Y., Zhang, J., and Cui, Q., “Metabolic adaption of ethanol-tolerant Clostridium thermocellum,” PLoS ONE 8:7p., (2013).
Birincil Dil en
Konular Mühendislik
Dergi Bölümü Biology
Yazarlar

Orcid: 0000-0002-7553-8927
Yazar: Asiye Gül BAYRAKCI OZDINGIS (Sorumlu Yazar)
Kurum: EGE UNIVERSITY, INSTITUTE OF SOLAR ENERGY
Ülke: Turkey


Yazar: Gunnur KOCAR
Kurum: EGE UNIVERSITY, INSTITUTE OF SOLAR ENERGY
Ülke: Turkey


Bibtex @araştırma makalesi { gujs386574, journal = {Gazi University Journal of Science}, issn = {}, eissn = {2147-1762}, address = {Gazi Üniversitesi}, year = {2018}, volume = {31}, pages = {1033 - 1046}, doi = {}, title = {Microorganism and Pretreatment Effect on Lignocellulosic Bioethanol Production}, key = {cite}, author = {KOCAR, Gunnur and BAYRAKCI OZDINGIS, Asiye Gül} }
APA BAYRAKCI OZDINGIS, A , KOCAR, G . (2018). Microorganism and Pretreatment Effect on Lignocellulosic Bioethanol Production. Gazi University Journal of Science, 31 (4), 1033-1046. Retrieved from http://dergipark.gov.tr/gujs/issue/40684/386574
MLA BAYRAKCI OZDINGIS, A , KOCAR, G . "Microorganism and Pretreatment Effect on Lignocellulosic Bioethanol Production". Gazi University Journal of Science 31 (2018): 1033-1046 <http://dergipark.gov.tr/gujs/issue/40684/386574>
Chicago BAYRAKCI OZDINGIS, A , KOCAR, G . "Microorganism and Pretreatment Effect on Lignocellulosic Bioethanol Production". Gazi University Journal of Science 31 (2018): 1033-1046
RIS TY - JOUR T1 - Microorganism and Pretreatment Effect on Lignocellulosic Bioethanol Production AU - Asiye Gül BAYRAKCI OZDINGIS , Gunnur KOCAR Y1 - 2018 PY - 2018 N1 - DO - T2 - Gazi University Journal of Science JF - Journal JO - JOR SP - 1033 EP - 1046 VL - 31 IS - 4 SN - -2147-1762 M3 - UR - Y2 - 2018 ER -
EndNote %0 Gazi University Journal of Science Microorganism and Pretreatment Effect on Lignocellulosic Bioethanol Production %A Asiye Gül BAYRAKCI OZDINGIS , Gunnur KOCAR %T Microorganism and Pretreatment Effect on Lignocellulosic Bioethanol Production %D 2018 %J Gazi University Journal of Science %P -2147-1762 %V 31 %N 4 %R %U
ISNAD BAYRAKCI OZDINGIS, Asiye Gül , KOCAR, Gunnur . "Microorganism and Pretreatment Effect on Lignocellulosic Bioethanol Production". Gazi University Journal of Science 31 / 4 (Aralık 2018): 1033-1046.