Yıl 2018, Cilt 31, Sayı 4, Sayfalar 1022 - 1031 2018-12-01

Lectin Histochemistry of Agrotis segetum Midgut Cells and Peritrophic Membrane

Naznoosh SHOMALI–MOGHADDAM [1] , Nursel GUL [2] , Ebru GOKALP OZKORKMAZ [3] , Aydin OZLUK [4]

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Midgut cell and peritrophic membranes of   grain pest Agrotis segetum larvae were examined by light microscope. Six biotinylated lectins were used in the assays by applying histochemical methods (Avidin-Biotin-Peroxidase). The aim of this study was to find lectins binding on peritrophic membranes if they were available as insecticidal agents. Results of the study indicated that lectins BPA (Bauhinia purpurea) and GS-I (Griffonia simplicifolia) strongly stained whereas, PNA (Pea Nut Agglutinin) and UEA-1 (Ulex europaeus) moderately stained the tissues. However, WGA (Wheat Germ Agglutinin) and Con-A (Canavalia ensiformis) stained the tissues weakly. The common feature of two binding proteins, BPA and GS-I lectin was binding to D- galactose. Our examinations revealed that   D-galactose mostly exists in A. segetum larvae midgut cell membranes and peritrophic membrane and this means BPA and GS-I lectins can be used as insecticidal agents against A. segetum.

Agrotis segetum larvae, Biotinylated lectins, Midgut cells, Peritrophic membrane
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Birincil Dil en
Konular Mühendislik
Dergi Bölümü Biology
Yazarlar

Yazar: Naznoosh SHOMALI–MOGHADDAM (Sorumlu Yazar)
Kurum: Ankara University, Ankara, Ankara, Turkey
Ülke: Turkey


Yazar: Nursel GUL
Kurum: Ankara University, Ankara, Ankara, Turkey
Ülke: Turkey


Yazar: Ebru GOKALP OZKORKMAZ
Kurum: Ankara Yildirim Beyazit University
Ülke: Turkey


Yazar: Aydin OZLUK
Kurum: Hitit University, Department of Biology, Çorum TURKEY
Ülke: Turkey


Bibtex @araştırma makalesi { gujs408091, journal = {Gazi University Journal of Science}, issn = {}, eissn = {2147-1762}, address = {Gazi Üniversitesi}, year = {2018}, volume = {31}, pages = {1022 - 1031}, doi = {}, title = {Lectin Histochemistry of Agrotis segetum Midgut Cells and Peritrophic Membrane}, key = {cite}, author = {GUL, Nursel and SHOMALI–MOGHADDAM, Naznoosh and GOKALP OZKORKMAZ, Ebru and OZLUK, Aydin} }
APA SHOMALI–MOGHADDAM, N , GUL, N , GOKALP OZKORKMAZ, E , OZLUK, A . (2018). Lectin Histochemistry of Agrotis segetum Midgut Cells and Peritrophic Membrane. Gazi University Journal of Science, 31 (4), 1022-1031. Retrieved from http://dergipark.gov.tr/gujs/issue/40684/408091
MLA SHOMALI–MOGHADDAM, N , GUL, N , GOKALP OZKORKMAZ, E , OZLUK, A . "Lectin Histochemistry of Agrotis segetum Midgut Cells and Peritrophic Membrane". Gazi University Journal of Science 31 (2018): 1022-1031 <http://dergipark.gov.tr/gujs/issue/40684/408091>
Chicago SHOMALI–MOGHADDAM, N , GUL, N , GOKALP OZKORKMAZ, E , OZLUK, A . "Lectin Histochemistry of Agrotis segetum Midgut Cells and Peritrophic Membrane". Gazi University Journal of Science 31 (2018): 1022-1031
RIS TY - JOUR T1 - Lectin Histochemistry of Agrotis segetum Midgut Cells and Peritrophic Membrane AU - Naznoosh SHOMALI–MOGHADDAM , Nursel GUL , Ebru GOKALP OZKORKMAZ , Aydin OZLUK Y1 - 2018 PY - 2018 N1 - DO - T2 - Gazi University Journal of Science JF - Journal JO - JOR SP - 1022 EP - 1031 VL - 31 IS - 4 SN - -2147-1762 M3 - UR - Y2 - 2018 ER -
EndNote %0 Gazi University Journal of Science Lectin Histochemistry of Agrotis segetum Midgut Cells and Peritrophic Membrane %A Naznoosh SHOMALI–MOGHADDAM , Nursel GUL , Ebru GOKALP OZKORKMAZ , Aydin OZLUK %T Lectin Histochemistry of Agrotis segetum Midgut Cells and Peritrophic Membrane %D 2018 %J Gazi University Journal of Science %P -2147-1762 %V 31 %N 4 %R %U
ISNAD SHOMALI–MOGHADDAM, Naznoosh , GUL, Nursel , GOKALP OZKORKMAZ, Ebru , OZLUK, Aydin . "Lectin Histochemistry of Agrotis segetum Midgut Cells and Peritrophic Membrane". Gazi University Journal of Science 31 / 4 (Aralık 2018): 1022-1031.