Yıl 2017, Cilt 21, Sayı 2, Sayfalar 227 - 238 2017-06-19

MicroRNA-Based Interference Applications in Plant Biotechnology
Bitki Biyoteknolojisi’nde MikroRNA Tabanlı İnterferans Uygulamaları

Fatma AYDINOĞLU [1] , Gizem AKTUĞ [2]

220 537

Plant biotechnology aims to improve desirable characters of plants by using modern genetic engineering tools. Recently, RNA interference (RNAi), which is a natural sequence-specific gene expression regulatory mechanism involving non-coding small RNAs like microRNA (miRNA), has gained importance as a beneficial tool for crop improvement. MiRNAs are identified as the key regulators in almost all biological and metabolic processes. Hereby, manipulating miRNA-based RNAi pathways offer a promising tool in the presence of successful applications on several fields of plant biotechnology, such as altering plant architecture, improving abiotic stress tolerance and biotic stress resistance, enrichment the nutritional value of plants, prolonging shelf life of fruits and vegetables, enhancing secondary metabolite production.  This review aims to provide the latest updates on plant miRNAs and applications of miRNA-based RNAi technology for crop improvement.

Bitki biyoteknolojisi, modern genetik mühendisliği araçlarını kullanarak bitkilerin istenilen karakterlerinin iyileştirilmesini amaçlar. Son yıllarda, protein kodlamayan küçük RNA’lardan olan mikroRNA (miRNA) genlerinin yer aldığı, doğal gen ifadesini düzenleyici mekanizma olan RNA interferans (RNAi), bitki geliştirilmesinde faydalı bir araç olarak önem kazanmıştır. miRNA’ların, hemen hemen bütün biyolojik ve metabolik işlevde anahtar düzenleyici role sahip oldukları ortaya konulmuştur. Hücresel yolakların miRNA tabanlı RNAi ile manipüle edilmesi ile bitki yapısının değiştirilmesi, abiyotik streslere toleransın ve biyotik streslere direncin geliştirilmesi, bitkilerin besin değerlerince zenginleştirilmesi, meyve ve sebzelerde raf ömrünün uzatılması ve sekonder metabolit üretiminin artırılması gibi daha pek çok bitki biyoteknolojisi alanında başarılı örneklerin varlığı, bu teknolojinin gelecek vaat eden bir araç olduğunu göstermektedir. Bu bağlamda, bu derleme, bitki miRNA genleri ve miRNA tabanlı RNAi teknolojisinin bitki iyileştirilmesinde uygulamalarına dair son gelişmeleri sunmayı amaçlamaktadır.

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Konular Tarım Bilimleri
Dergi Bölümü Derleme Makaleleri

Yazar: Fatma AYDINOĞLU
Kurum: Gebze Teknik Üniversitesi, Temel Bilimler Fakültesi, Moleküler Biyoloji ve Genetik Bölümü
Ülke: Turkey

Yazar: Gizem AKTUĞ
Kurum: Gebze Teknik Üniversitesi, Temel Bilimler Fakültesi, Moleküler Biyoloji ve Genetik Bölümü
Ülke: Turkey

Bibtex @derleme { harranziraat321171, journal = {Harran Tarım ve Gıda Bilimleri Dergisi}, issn = {2148-5003}, eissn = {2587-1358}, address = {Harran Üniversitesi}, year = {2017}, volume = {21}, pages = {227 - 238}, doi = {10.29050/harranziraat.321171}, title = {Bitki Biyoteknolojisi’nde MikroRNA Tabanlı İnterferans Uygulamaları}, key = {cite}, author = {AKTUĞ, Gizem and AYDINOĞLU, Fatma} }
APA AYDINOĞLU, F , AKTUĞ, G . (2017). Bitki Biyoteknolojisi’nde MikroRNA Tabanlı İnterferans Uygulamaları. Harran Tarım ve Gıda Bilimleri Dergisi, 21 (2), 227-238. DOI: 10.29050/harranziraat.321171
MLA AYDINOĞLU, F , AKTUĞ, G . "Bitki Biyoteknolojisi’nde MikroRNA Tabanlı İnterferans Uygulamaları". Harran Tarım ve Gıda Bilimleri Dergisi 21 (2017): 227-238 <http://dergipark.gov.tr/harranziraat/issue/29825/321171>
Chicago AYDINOĞLU, F , AKTUĞ, G . "Bitki Biyoteknolojisi’nde MikroRNA Tabanlı İnterferans Uygulamaları". Harran Tarım ve Gıda Bilimleri Dergisi 21 (2017): 227-238
RIS TY - JOUR T1 - Bitki Biyoteknolojisi’nde MikroRNA Tabanlı İnterferans Uygulamaları AU - Fatma AYDINOĞLU , Gizem AKTUĞ Y1 - 2017 PY - 2017 N1 - doi: 10.29050/harranziraat.321171 DO - 10.29050/harranziraat.321171 T2 - Harran Tarım ve Gıda Bilimleri Dergisi JF - Journal JO - JOR SP - 227 EP - 238 VL - 21 IS - 2 SN - 2148-5003-2587-1358 M3 - doi: 10.29050/harranziraat.321171 UR - http://dx.doi.org/10.29050/harranziraat.321171 Y2 - 2017 ER -
EndNote %0 Harran Tarım ve Gıda Bilimleri Dergisi Bitki Biyoteknolojisi’nde MikroRNA Tabanlı İnterferans Uygulamaları %A Fatma AYDINOĞLU , Gizem AKTUĞ %T Bitki Biyoteknolojisi’nde MikroRNA Tabanlı İnterferans Uygulamaları %D 2017 %J Harran Tarım ve Gıda Bilimleri Dergisi %P 2148-5003-2587-1358 %V 21 %N 2 %R doi: 10.29050/harranziraat.321171 %U 10.29050/harranziraat.321171