Yıl 2018, Cilt 5, Sayı 2, Sayfalar 140 - 148 2018-04-05

Extraction, purification, antioxidant properties and stability conditions of phytomelanin pigment on the sunflower seeds

Yuksel Keles [1] , Önder Özdemir [2]

14 32

Phytomelanin pigment, a rare secondary matabolite in plants, has potential for use in the cosmetic and food sectors. This pigment has antioxidant, antimicrobial and ultraviolet (UV) light absorbing properties, so it can be used as natural food coloring and in the field of cosmetics. In this study, extraction, purification, antioxidant properties and stability conditions of phytomelanin pigment found in sunflower (Helianthus annuus L.) seed coat were investigated. NaOH, KOH and NH4OH solutions were tested at different concentrations for extraction. It has been determined that the most suitable solvent for extraction is 0.3 M NaOH. The purification process involves precipitation with HCl followed by washing with ethyl alcohol, ethyl acetate and acetone. The findings show that the proportion of phytomelanin in sunflower seeds is 1.95% and that the antioxidant capacity is 9.8% ascorbic acid equivalent. The purity degree of the purified phytomelanin pigment and that of the synthetic phytomelanin pigment were compared by thin layer chromatography (TLC). Chromatography findings have shown that the purification performance is quite high. It was determined that the pigment was slowly deteriorated in temperature and light, and was not affected by air. Consequently, sunflower seed coat can be a convenient and economical source of producing pure phytomelanin for industrial use.
Helianthus annuus, rare pigments, seconder metabolite, seed coat, TLC
  • Francis, F.J. (1999) Colorants. Eagan, Minnesota, pp.144
  • Assous, M.T.M., Abdel-Hady, M.M., Medany, G.M. (2014) Evaluation of red pigment extracted from purple carrots and its utilization as antioxidant and natural food colorants. Annals of Agricultural Science, 59(1): 1-7.
  • Shujing, S., Zhang, X., Sun, S., Zhang, L., Shan, S., Zhu, H. (2015) Production of natural melanin by Auricularia auricula and study on its molecular structure. Food Chemistry, 190: 801-807.
  • De-Paula, O.C., Marzinek, J., Oliveira, D.M.T. (2013) The role of fibres and the hypodermis in Compositae melanin secretion. Micron, 44: 312-316.
  • Hendry, G.A.F., Houghton, J.D. (1996) Natural Food Colorants. Blackie Academic & Professional, pp. 348.
  • Pandey, A.K., Dhakal, M.R. (2001) Phytomelanin in Compositae. Current Science, Vol. 80(8): 933-940.
  • Pintea, A.M. (2008) Other Natural Pigments. Food Colorants Chemical and Functional Properties, (Editör: Socaciu, C. ), CRC, Florida, pp. 102-119.
  • Sava V.M., Yang S., Hong M., Yang P., Huang G.S. (2001) Isolation and characterization of melanic pigments derived from tea and tea polyphenols. Food Chemsitry. 73: 177-184.
  • Jana, B.K., Mukherjee, S.K. (2014) Notes on the distribution of phtomelanin layer in higher plants – A short communication. Journal of Pharmaceutical Biology, 4(3): 131-132.
  • Kannan, P., Ganjewala, D. (2009) Preliminary characterization of melanin isolated from fruits and seeds of Nyctanthes arbor-tristis. Journal of Scientific Research, 1(3): 655-661.
  • European Patent Office. (2003) Biologically active fraction of vegetable melanin, process for its production and its use. Bulletin 2003/43.
  • Rajalakshmi, D., Narasimhan, S. (1995) Food Antioxidants: Sources and Methods of Evaluation. Food Antioxidants, (Editör; Madhavi, D.L., Deshpande, S.S., Salunkhe, D.K.) Marcel Dekker Inc., New York, pp. 65-159.
  • Girgin M., Ceylan Ö. (2012) Streptomyces sp. OC 73-9 izolatı tarafından üretilen melanin pigmentinin antioksidan aktivitesinin belirlenmesi. 21. Ulusal Biyoloji Kongresi, 3-7 Eylül, p.772. İzmir, Turkey.
  • Kiran GS, Dhasayan A, Lipton AN, Selvin J, Arasu MV, Al-Dhabi NA. Melanin-templated rapid synthesis of silver nanostructures. Journal of Nanobiotechnology. 2014;12:8. doi: 10.1186/1477-3155-12-18.
  • Madhusudhan D.N., Mazhari Z.B.B., Dastager S.G., Agsar D. (2014) Production and cytotoxicity of extracellular insoluble and droplets of soluble melanin by Streptomyces lusitanus DMZ-3. Biomed Research International, Vol. 2014, Article ID 306895, 11 pages
  • Davies, K.M. (2004) Plant Pigments and Their Manipulation, Annual Plant Reviews Volume 14, Blackwel & CRC, Canada, pp. 240.
  • Kumar, J.B., Kumar, M.S. (2013) A general idea about phytomelanin layer in some Compositae. Indian Journal of Plant Sciences, 2013 Vol. 2 (4): 44-48.
  • Pokorny J., Yanishlieva N., Gordon M. (2001) Antioxidants In Food. Woodhead Publishing Ltd, Cambridge, pp. 380.
  • Hung, Y.C., Sava, V.M., Makan, S.Y., Chen, T.H.J., Hong, M.Y, Huang, G.S. (2002) Antioxidant activity of melanins derived from tea: comparison between different oxidative states. Food Chemistry, 78: 233-240.
  • Delgado-Vargas, F., Paredes-Lopez, O. (2003) Natural Colorants for Food and Nutraceutical Uses. CRC, Florida, pp. 327.
Birincil Dil en
Konular Biyoloji
Yayımlanma Tarihi July
Dergi Bölümü Makaleler
Yazarlar

Orcid: 0000-0001-8651-8385
Yazar: Yuksel Keles (Sorumlu Yazar)
E-posta: ykeles@mersin.edu.tr
Kurum: Mersin University, Education Faculty, Biology Education Department Yenisehir / Mersin
Ülke: Turkey


Yazar: Önder Özdemir
E-posta: o.ozdemirr@gmail.com
Ülke: Turkey


Bibtex @araştırma makalesi { ijsm377470, journal = {International Journal of Secondary Metabolite}, issn = {}, address = {İzzet KARA}, year = {2018}, volume = {5}, pages = {140 - 148}, doi = {10.21448/ijsm.377470}, title = {Extraction, purification, antioxidant properties and stability conditions of phytomelanin pigment on the sunflower seeds}, key = {cite}, author = {Özdemir, Önder and Keles, Yuksel} }
APA Keles, Y , Özdemir, Ö . (2018). Extraction, purification, antioxidant properties and stability conditions of phytomelanin pigment on the sunflower seeds. International Journal of Secondary Metabolite, 5 (2), 140-148. DOI: 10.21448/ijsm.377470
MLA Keles, Y , Özdemir, Ö . "Extraction, purification, antioxidant properties and stability conditions of phytomelanin pigment on the sunflower seeds". International Journal of Secondary Metabolite 5 (2018): 140-148 <http://dergipark.gov.tr/ijsm/issue/34119/377470>
Chicago Keles, Y , Özdemir, Ö . "Extraction, purification, antioxidant properties and stability conditions of phytomelanin pigment on the sunflower seeds". International Journal of Secondary Metabolite 5 (2018): 140-148
RIS TY - JOUR T1 - Extraction, purification, antioxidant properties and stability conditions of phytomelanin pigment on the sunflower seeds AU - Yuksel Keles , Önder Özdemir Y1 - 2018 PY - 2018 N1 - doi: 10.21448/ijsm.377470 DO - 10.21448/ijsm.377470 T2 - International Journal of Secondary Metabolite JF - Journal JO - JOR SP - 140 EP - 148 VL - 5 IS - 2 SN - -2148-6905 M3 - doi: 10.21448/ijsm.377470 UR - http://dx.doi.org/10.21448/ijsm.377470 Y2 - 2018 ER -
EndNote %0 International Journal of Secondary Metabolite Extraction, purification, antioxidant properties and stability conditions of phytomelanin pigment on the sunflower seeds %A Yuksel Keles , Önder Özdemir %T Extraction, purification, antioxidant properties and stability conditions of phytomelanin pigment on the sunflower seeds %D 2018 %J International Journal of Secondary Metabolite %P -2148-6905 %V 5 %N 2 %R doi: 10.21448/ijsm.377470 %U 10.21448/ijsm.377470