Yıl 2018, Cilt 5, Sayı 2, Sayfalar 67 - 74 2018-03-01

Einkorn (Triticum monococcum ssp. monococcum) in vitro propagation sterilization protocol

Mehmet Örgeç Örgeç [1] , Fatma Pehlivan Karakaş [2] , Günce Şahin [3] , Ferdi Ağıl [4] , Nusret Zencirci [5]

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Einkorn is one of the oldest and important wheat species because of its increasing economic value, agronomical technical properties, and health and sanitary effects. Therefore, a faster tissue culture production protocol which may ensure a fast einkorn production is desired.  The tissue culture process first requires an efficient sterilization technique of the explant to eliminate contamination factors in this hulled species. In this study, we, thus, aimed to determine the effective einkorn sterilization process for the removal of contaminating microorganisms without the loss of germination capacity and viability. We tested 22 following sterilant combinations with different concentration and exposure time: Ethanol, Commercial Bleach, Tween 20, Mercury (II) Chloride, and Sterile Distilled Water. On the other hand, obtained the data were analyzed by one-way ANOVA and Duncan test, where p≤0.05 was accepted significant. Forty percent commercial bleach which were applied fifteen minutes eliminated all microorganisms with a 92.6% seed germination. The second-best technique was one minute exposure of 70% ethanol which were followed by 10 minutes exposure of 20% commercial bleach. This assured up to 89% germination and no contamination. The worst results came from the mercury (II) chloride with 0% contamination and ≤ 25% germination rate. Mercury (II) Chloride was both highly toxic on the seeds and reduced the contamination. Commercial Bleach and Ethanol Chemicals were less toxic while providing an acceptable sterilization.

Contamination, Einkorn wheat, Germination, Seed sterilization, Tissue culture
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Birincil Dil en
Konular Biyoloji
Yayımlanma Tarihi July
Dergi Bölümü Makaleler
Yazarlar

Orcid: orcid.org/0000-0002-9446-7538
Yazar: Mehmet Örgeç Örgeç (Sorumlu Yazar)
E-posta: mehmetorgec@gmail.com

Orcid: orcid.org/0000-0001-5245-6294
Yazar: Fatma Pehlivan Karakaş
E-posta: fatmapehlivankarakas@gmail.com

Orcid: orcid.org/0000-0003-0060-259X
Yazar: Günce Şahin
E-posta: guncesahin@gmail.com

Orcid: orcid.org/0000-0003-1685-6711
Yazar: Ferdi Ağıl
E-posta: ferdi.agill@gmail.com

Orcid: orcid.org/0000-0003-3460-7575
Yazar: Nusret Zencirci
E-posta: nzencirci@yahoo.com

Bibtex @araştırma makalesi { ijsm399094, journal = {International Journal of Secondary Metabolite}, issn = {}, address = {İzzet KARA}, year = {2018}, volume = {5}, pages = {67 - 74}, doi = {10.21448/ijsm.399094}, title = {Einkorn (Triticum monococcum ssp. monococcum) in vitro propagation sterilization protocol}, key = {cite}, author = {Zencirci, Nusret and Örgeç, Mehmet Örgeç and Şahin, Günce and Ağıl, Ferdi and Pehlivan Karakaş, Fatma} }
APA Örgeç, M , Pehlivan Karakaş, F , Şahin, G , Ağıl, F , Zencirci, N . (2018). Einkorn (Triticum monococcum ssp. monococcum) in vitro propagation sterilization protocol. International Journal of Secondary Metabolite, 5 (2), 67-74. DOI: 10.21448/ijsm.399094
MLA Örgeç, M , Pehlivan Karakaş, F , Şahin, G , Ağıl, F , Zencirci, N . "Einkorn (Triticum monococcum ssp. monococcum) in vitro propagation sterilization protocol". International Journal of Secondary Metabolite 5 (2018): 67-74 <http://dergipark.gov.tr/ijsm/issue/34119/399094>
Chicago Örgeç, M , Pehlivan Karakaş, F , Şahin, G , Ağıl, F , Zencirci, N . "Einkorn (Triticum monococcum ssp. monococcum) in vitro propagation sterilization protocol". International Journal of Secondary Metabolite 5 (2018): 67-74
RIS TY - JOUR T1 - Einkorn (Triticum monococcum ssp. monococcum) in vitro propagation sterilization protocol AU - Mehmet Örgeç Örgeç , Fatma Pehlivan Karakaş , Günce Şahin , Ferdi Ağıl , Nusret Zencirci Y1 - 2018 PY - 2018 N1 - doi: 10.21448/ijsm.399094 DO - 10.21448/ijsm.399094 T2 - International Journal of Secondary Metabolite JF - Journal JO - JOR SP - 67 EP - 74 VL - 5 IS - 2 SN - -2148-6905 M3 - doi: 10.21448/ijsm.399094 UR - http://dx.doi.org/10.21448/ijsm.399094 Y2 - 2018 ER -
EndNote %0 International Journal of Secondary Metabolite Einkorn (Triticum monococcum ssp. monococcum) in vitro propagation sterilization protocol %A Mehmet Örgeç Örgeç , Fatma Pehlivan Karakaş , Günce Şahin , Ferdi Ağıl , Nusret Zencirci %T Einkorn (Triticum monococcum ssp. monococcum) in vitro propagation sterilization protocol %D 2018 %J International Journal of Secondary Metabolite %P -2148-6905 %V 5 %N 2 %R doi: 10.21448/ijsm.399094 %U 10.21448/ijsm.399094