Yıl 2018, Cilt 5, Sayı 2, Sayfalar 156 - 162 2018-05-08

Effect of Phytohormones of Kinetin and Epibrassinolide on Content and Intracellular Localization of Glucosides and Free Amino Acids in Pea Plants Cells (Pisum sativum L.)

Antonina N. Ershova [1] , Natalia Vinokurova [2]

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During injection of kinetin and epibrassinolide in pea seedlings (Pisum sativum L.) an increase of content of specific for this compound isosuccinimide-β-glucoside (IS-glucoside) for 15-30%, ethyl-β-glucoside for 40-80%, GABA free amino acids, glutamate and alanine only under kinetin for 15-20% was observed. The field experiments showed an increase of aglycone content but the amount of IS-glucoside decreased at first and then increased 14 days after plant treatment with phytohormones. It was shown that effect of phytohormones on content of IS-glucosides and free amino acids was dependent on action time and age of plant. By using membrane trophic compound of DMSO it was discovered that in pea seedlings cells the vacuolar fund of IS-glucoside was 70% of initial and aglycone with γ-aminobutyric acid as its precursor were localized in cytoplasm. Aspartate and glutamate were almost equally distributed between cytoplasmic and vacuolar cell funds. It is suggested that reactions of pyrrolidone structure of IS-glucoside aglycone formation and synthesis of the glucoside were processed in cytoplasm. Further IS-glucoside was transferred and stored in vacuoles of pea seedlings cells by contrast with ethyl-β-glucoside mainly localized in cytoplasm.

GABA, Ethyl-β-glucoside, Isosuccinimide-β-glucoside, Vacuole
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Birincil Dil en
Konular Biyoloji
Yayımlanma Tarihi July
Dergi Bölümü Makaleler
Yazarlar

Orcid: orcid.org/0000-0001-7148-6800
Yazar: Antonina N. Ershova (Sorumlu Yazar)
Kurum: Voronezh State Pedagogical University
Ülke: Russian Federation


Yazar: Natalia Vinokurova
Kurum: Voronezh State Pedagogical University
Ülke: Russian Federation


Bibtex @araştırma makalesi { ijsm422077, journal = {International Journal of Secondary Metabolite}, issn = {}, eissn = {2148-6905}, address = {İzzet KARA}, year = {2018}, volume = {5}, pages = {156 - 162}, doi = {10.21448/ijsm.422077}, title = {Effect of Phytohormones of Kinetin and Epibrassinolide on Content and Intracellular Localization of Glucosides and Free Amino Acids in Pea Plants Cells (Pisum sativum L.)}, key = {cite}, author = {Vinokurova, Natalia and Ershova, Antonina N.} }
APA Ershova, A , Vinokurova, N . (2018). Effect of Phytohormones of Kinetin and Epibrassinolide on Content and Intracellular Localization of Glucosides and Free Amino Acids in Pea Plants Cells (Pisum sativum L.). International Journal of Secondary Metabolite, 5 (2), 156-162. DOI: 10.21448/ijsm.422077
MLA Ershova, A , Vinokurova, N . "Effect of Phytohormones of Kinetin and Epibrassinolide on Content and Intracellular Localization of Glucosides and Free Amino Acids in Pea Plants Cells (Pisum sativum L.)". International Journal of Secondary Metabolite 5 (2018): 156-162 <http://dergipark.gov.tr/ijsm/issue/34119/422077>
Chicago Ershova, A , Vinokurova, N . "Effect of Phytohormones of Kinetin and Epibrassinolide on Content and Intracellular Localization of Glucosides and Free Amino Acids in Pea Plants Cells (Pisum sativum L.)". International Journal of Secondary Metabolite 5 (2018): 156-162
RIS TY - JOUR T1 - Effect of Phytohormones of Kinetin and Epibrassinolide on Content and Intracellular Localization of Glucosides and Free Amino Acids in Pea Plants Cells (Pisum sativum L.) AU - Antonina N. Ershova , Natalia Vinokurova Y1 - 2018 PY - 2018 N1 - doi: 10.21448/ijsm.422077 DO - 10.21448/ijsm.422077 T2 - International Journal of Secondary Metabolite JF - Journal JO - JOR SP - 156 EP - 162 VL - 5 IS - 2 SN - -2148-6905 M3 - doi: 10.21448/ijsm.422077 UR - http://dx.doi.org/10.21448/ijsm.422077 Y2 - 2018 ER -
EndNote %0 International Journal of Secondary Metabolite Effect of Phytohormones of Kinetin and Epibrassinolide on Content and Intracellular Localization of Glucosides and Free Amino Acids in Pea Plants Cells (Pisum sativum L.) %A Antonina N. Ershova , Natalia Vinokurova %T Effect of Phytohormones of Kinetin and Epibrassinolide on Content and Intracellular Localization of Glucosides and Free Amino Acids in Pea Plants Cells (Pisum sativum L.) %D 2018 %J International Journal of Secondary Metabolite %P -2148-6905 %V 5 %N 2 %R doi: 10.21448/ijsm.422077 %U 10.21448/ijsm.422077
ISNAD Ershova, Antonina N. , Vinokurova, Natalia . "Effect of Phytohormones of Kinetin and Epibrassinolide on Content and Intracellular Localization of Glucosides and Free Amino Acids in Pea Plants Cells (Pisum sativum L.)". International Journal of Secondary Metabolite 5 / 2 (Mayıs 2018): 156-162. http://dx.doi.org/10.21448/ijsm.422077