Yıl 2018, Cilt 5, Sayı 3, Sayfalar 210 - 216 2018-09-15

Effects of NaCl applications on root growth and secondary metabolite production in madder (Rubia tinctorum L.) root cultures

Özlem Aras Aşci [1] , Tunhan Demirci [2] , Nilgün Göktürk Baydar [3]

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Madder (Rubia tinctorum L.) is a valuable plant rich in anthraquinones having dying properties and biological activities. This study was carried out to determine the effect of sodium chloride (NaCl) applications on the root growth and secondary metabolite accumulation in adventitious roots of madder. For this aim, adventitious roots derived from stem explants in in vitro conditions were cultured in MS medium containing different concentrations of NaCl (0, 1, 2, 3 and 4 g/l) for 7 days. Then roots were evaluated in terms of root growth index, total AQ, alizarin, purpurin and total phenolic contents. Based on the results, root growth decreased in line with the elevating level of NaCl while secondary metabolite accumulation significantly increased with NaCl applications compared to the controls. It was determined that NaCl at 3 g/l concentration was the most effective application in terms of total AQ, alizarin, purpurin and phenolic accumulation.

Rubia tinctorum, root culture, in vitro, sodium chloride, secondary metabolite
  • Karuppusamy S. (2009) A review on trends in production of secondary metabolites from higher plants by in vitro tissue, organ and cell cultures. Journal of Medicinal Plants Research, 3(13), 1222-1239.
  • Hussain M.S., Fareed S., Saba Ansari M., Rahman A., Ahmad I.Z., Saeed M. (2012) Current approaches toward production of secondary plant metabolites. Journal of Pharmacy & Bioallied Sciences, 4(1), 10-20.
  • Rao S.R., Ravishankar G.A. (2002) Plant cell cultures: chemical factories of secondary metabolites. Biotechnology Advances, 20(2), 101-153.
  • Baydar H., Karadoğan T. (2006) Agronomic potential and industrial value of madder (Rubia tinctorum L.) as a dye crop. Turkish Journal of Agriculture and Forestry, 30(4), 287-293.
  • Kinnosuko O., Takahito I., Keiko K. (1991) Madders dye, In: Atsuchi, K. (Ed.), Plant Cell Culture in Japan, CMC. Japan, p. 138-142.
  • Kawasaki Y., Yukihiro G.O.D.A., Yoshihira K. (1992) The mutagenic constituents of Rubia tinctorum. Chemical and Pharmaceutical Bulletin, 40(6), 1504-1509.
  • Kalyoncu F., Çetin B., Sağlam H. (2006) Antimicrobial activity of common madder (Rubia tinctorum L.). Phytotheraphy Research, 20, 490-492.
  • Siva R., Palackan M.G., Maimoon L., Geetha T., Bhakta D., Balamurugan P., Rajanarayanan S. (2011) Evaluation of antibacterial, antifungal, and antioxidant properties of some food dyes. Food Science and Biotechnology, 20(1), 7-13.
  • Lajkó E., Bányai P., Zámbó Z., Kursinszki L., Szőke É., Kőhidai L. (2015) Targeted tumor therapy by Rubia tinctorum L.: analytical characterization of hydroxyanthraquinones and investigation of their selective cytotoxic, adhesion and migration modulator effects on melanoma cell lines (A2058 and HT168-M1). Cancer Cell International, 15(1), 119.
  • Orban N., Boldizar I., Szücs Z., Bela D. (2008) Influence of different elicitors on the synthesis of antraquinone derivatives in Rubia tinctorum L. cell suspension cultures. Dyes and Pigments, 77, 249-257.
  • Hussein E.A., Aqlan E.M. (2011) Effect of mannitol and sodium chloride on some total secondary metabolites of fenugreek calli cultured in vitro. Plant Tissue Culture and Biotechnology, 21(1), 35-43.
  • Nazif W., Perveen S., Saleem I. (2006) Status of micronutrients in soils of district Bhimber (Azad Jammu and Kashmir). Journal of Agricultural and Biological Science, 1(2), 35-40.
  • Fatima S., Mujib A., Tonk D. (2015) NaCl amendment improves vinblastine and vincristine synthesis in Catharanthusroseus: a case of stress signalling as evidenced by antioxidant enzymes activities. Plant Cell, Tissue and Organ Culture (PCTOC), 121(2), 445-458.
  • Ni J., Yang X., Zhu J., Liu Z., Ni Y., Wu H., Zhang H., Liu T. (2015) Salinity-induced metabolic profile changes in Nitraria tangutorum Bobr. suspension cells. Plant Cell, Tissue and Organ Culture (PCTOC), 122(1), 239-248.
  • Nartop P., Akay Ş., Gürel A. (2017) Rubia tinctorum L. hücre kültürlerinde tuz stresi ve inokülasyon oranlarının etkileri, Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 21(3), 1-1.
  • Oksana S., Marian B., Mahendra R., Bo S.H. (2012) Plant phenolic compounds for food, pharmaceutical and cosmetiсs production. Journal of Medicinal Plants Research, 6(13), 2526-2539.
  • Kubota H., Sato K., Yamada T., Maitani T. (1995) Phytochelatins (class lll metallothioneins) and their desglycyl peptides induced by cadmium in normal root cultures of Rubia tinctorum. Plant Sciene, 106(2), 157-166.
  • Murashige T., Skoog F. (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497.
  • Schulte U., El-Shagi H., Zenk M.N. (1984) Optimization of Rubiaceae species in cell culture for the production of anthraquinones. Plant Cell Reports, 3, 51-54.
  • Shin S.H. (1989) Studies on the production of anthraquinone derivatives by tissue culture of Rubia species. Archives Pharmacal Research, 12, 99-102.
  • Singleton V.L., Rossi J.R. (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid. American Journal of Enology and Viticulture, 16, 144-158.
  • Nazif N.M., Rady M.R., El-Nasr M.S. (2000) Stimulation of anthraquinone production in suspension cultures of Cassia acutifolia by salt stress. Fitoterapia, 71(1), 34-40.
  • Jeong G.T., Park D.H. (2006) Enhanced secondary metabolite biosynthesis by elicitation in transformed plant root system: effect of abiotic elicitors. Applied Biochemistry and Biotechnology, 129-132.
  • Munns R. (2002) Comparative physiology of salt and water stress. Plant, Cell & Environment, 25(2), 239-250.
  • Akula R., Ravishankar G.A. (2011) Influence of abiotic stress signals on secondary metabolites in plants. Plant Signaling & Behavior, 6(11), 1720-1731.
  • Lindsey K., Yeoman M.M. (1983) The relationship between growth rate, differentiation and alkaloid accumulation in cell cultures. Journal of Experimental Botany, 34(8), 1055-1065.
  • Ali R.M., Abbas H.M. (2003) Response of salt stressed barley seedlings to phenylurea. Plant Soil and Environment, 49(4), 158-162.
  • Jaleel C.A., Sankar B., Sridharan R., Panneerselvam R. (2008) Soil salinity alters growth, chlorophyll content, and secondary metabolite accumulation in Catharanthus roseus. Turkish Journal of Biology, 32(2), 79-83.
  • Mahajan S., Tuteja N. (2005) Cold, salinity and drought stresses: an overview. Archives of Biochemistry and Biophysics, 444(2), 139-158.
  • Naik P.M., Al–Khayri J.M. (2016) Abiotic and biotic elicitors–role in secondary metabolites production through in vitro culture of medicinal plants: A review, Journal of Advanced Research in Biotechnology, 1(2), 1-7.
  • Taiz L., Zeiger E. (2006) Plant physiology, 4th edn. Sinauer Associates Inc. Publishers, Massachusetts.
  • Selmar D. (2008) Potential of salt and drought stress to increase pharmaceutical significant secondary compounds in plants. Landbauforschung Volkenrode Agriculture and Forestry Research, 58, 139-144.
Birincil Dil en
Konular Biyoloji
Yayımlanma Tarihi September
Dergi Bölümü Makaleler
Yazarlar

Orcid: 0000-0002-8940-7514
Yazar: Özlem Aras Aşci (Sorumlu Yazar)
Kurum: SULEYMAN DEMIREL UNIVERSITY
Ülke: Turkey


Orcid: orcid.org/0000-0001-8516-911X
Yazar: Tunhan Demirci

Orcid: orcid.org/0000-0002-5482-350X
Yazar: Nilgün Göktürk Baydar
Kurum: SULEYMAN DEMIREL UNIVERSITY
Ülke: Turkey


Bibtex @araştırma makalesi { ijsm453016, journal = {International Journal of Secondary Metabolite}, issn = {}, eissn = {2148-6905}, address = {İzzet KARA}, year = {2018}, volume = {5}, pages = {210 - 216}, doi = {10.21448/ijsm.453016}, title = {Effects of NaCl applications on root growth and secondary metabolite production in madder (Rubia tinctorum L.) root cultures}, key = {cite}, author = {Aras Aşci, Özlem and Demirci, Tunhan and Göktürk Baydar, Nilgün} }
APA Aras Aşci, Ö , Demirci, T , Göktürk Baydar, N . (2018). Effects of NaCl applications on root growth and secondary metabolite production in madder (Rubia tinctorum L.) root cultures. International Journal of Secondary Metabolite, 5 (3), 210-216. DOI: 10.21448/ijsm.453016
MLA Aras Aşci, Ö , Demirci, T , Göktürk Baydar, N . "Effects of NaCl applications on root growth and secondary metabolite production in madder (Rubia tinctorum L.) root cultures". International Journal of Secondary Metabolite 5 (2018): 210-216 <http://dergipark.gov.tr/ijsm/issue/37294/453016>
Chicago Aras Aşci, Ö , Demirci, T , Göktürk Baydar, N . "Effects of NaCl applications on root growth and secondary metabolite production in madder (Rubia tinctorum L.) root cultures". International Journal of Secondary Metabolite 5 (2018): 210-216
RIS TY - JOUR T1 - Effects of NaCl applications on root growth and secondary metabolite production in madder (Rubia tinctorum L.) root cultures AU - Özlem Aras Aşci , Tunhan Demirci , Nilgün Göktürk Baydar Y1 - 2018 PY - 2018 N1 - doi: 10.21448/ijsm.453016 DO - 10.21448/ijsm.453016 T2 - International Journal of Secondary Metabolite JF - Journal JO - JOR SP - 210 EP - 216 VL - 5 IS - 3 SN - -2148-6905 M3 - doi: 10.21448/ijsm.453016 UR - http://dx.doi.org/10.21448/ijsm.453016 Y2 - 2018 ER -
EndNote %0 International Journal of Secondary Metabolite Effects of NaCl applications on root growth and secondary metabolite production in madder (Rubia tinctorum L.) root cultures %A Özlem Aras Aşci , Tunhan Demirci , Nilgün Göktürk Baydar %T Effects of NaCl applications on root growth and secondary metabolite production in madder (Rubia tinctorum L.) root cultures %D 2018 %J International Journal of Secondary Metabolite %P -2148-6905 %V 5 %N 3 %R doi: 10.21448/ijsm.453016 %U 10.21448/ijsm.453016
ISNAD Aras Aşci, Özlem , Demirci, Tunhan , Göktürk Baydar, Nilgün . "Effects of NaCl applications on root growth and secondary metabolite production in madder (Rubia tinctorum L.) root cultures". International Journal of Secondary Metabolite 5 / 3 (Eylül 2018): 210-216. http://dx.doi.org/10.21448/ijsm.453016