Yıl 2018, Cilt 5, Sayı 4, Sayfalar 343 - 352 2018-12-29

The phenolic content, antioxidant and cytotoxic activities of Origanum sipyleum from Turkey

Arzu Kaska [1]

11 28

Origanum sipyleum is a medicinal plant species belonging to the genus Origanum and traditionally vegetative parts of this plant have been used as medicinal tea or food additives. The current study has been designed to examine the antioxidant, cytotoxic activities and total phenolic, flavonoid and tannin contents as well as the chemical composition of the ethanol extract of O. sipyleum. The antioxidant capacity of O. sipyleum was determined using six complementary methods, β-carotene/linoleic acid and phosphomolybdenum test systems, radical scavenging (ABTS and DPPH), metal chelating and reducing power assays. The phenolics were identified using HPLC. A brine shrimp (Artemia salina L.) lethality test was used for determining cytotoxic activity. The ethanol extract exhibited high DPPH free radical scavenging (DPPH, IC50:102.75 µg/mL), ABTS radical scavenging (ABTS, IC50:88.64 µg/mL), metal chelating (20.68 %) and reducing power capacity (0.51 mg/mL). The antioxidant activities of the O. sipyleum with β-carotene/Linoleic acid and phosphomolybdenum were calculated as 85.59 % and 62.95 µg/mg respectively. The phenolic contents of the ethanol extract were evaluated using HPLC and determined major phenolics: caffeic acid, epicatechin and 2,5 dihydroxybenzoic. Furthermore, to gether with cytotoxic activity (LC50, 327.414 µg/mL) O. sipyleum is also rich in total phenolic, flavonoid and tannin contents were 203.57 ± 4.62 mgGAE/g, 46.98 ± 0.34 mgQE/g and 34.55 ± 0.56 mgCE/g respectively. These results could provide addition information for the potential use of this medicinal plant as a food ingredient and as a natural antioxidant in the diet, as well as for the pharmaceutical industry.

Cytotoxic, DPPH, HPLC, Natural antioxidant, Origanum sipyleum
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Birincil Dil en
Konular Biyoloji
Yayımlanma Tarihi December
Dergi Bölümü Makaleler
Yazarlar

Orcid: 0000-0002-0166-1818
Yazar: Arzu Kaska (Sorumlu Yazar)
Kurum: PAMUKKALE UNIVERSITY
Ülke: Turkey


Bibtex @araştırma makalesi { ijsm468418, journal = {International Journal of Secondary Metabolite}, issn = {}, eissn = {2148-6905}, address = {İzzet KARA}, year = {2018}, volume = {5}, pages = {343 - 352}, doi = {10.21448/ijsm.468418}, title = {The phenolic content, antioxidant and cytotoxic activities of Origanum sipyleum from Turkey}, key = {cite}, author = {Kaska, Arzu} }
APA Kaska, A . (2018). The phenolic content, antioxidant and cytotoxic activities of Origanum sipyleum from Turkey. International Journal of Secondary Metabolite, 5 (4), 343-352. DOI: 10.21448/ijsm.468418
MLA Kaska, A . "The phenolic content, antioxidant and cytotoxic activities of Origanum sipyleum from Turkey". International Journal of Secondary Metabolite 5 (2018): 343-352 <http://dergipark.gov.tr/ijsm/issue/39967/468418>
Chicago Kaska, A . "The phenolic content, antioxidant and cytotoxic activities of Origanum sipyleum from Turkey". International Journal of Secondary Metabolite 5 (2018): 343-352
RIS TY - JOUR T1 - The phenolic content, antioxidant and cytotoxic activities of Origanum sipyleum from Turkey AU - Arzu Kaska Y1 - 2018 PY - 2018 N1 - doi: 10.21448/ijsm.468418 DO - 10.21448/ijsm.468418 T2 - International Journal of Secondary Metabolite JF - Journal JO - JOR SP - 343 EP - 352 VL - 5 IS - 4 SN - -2148-6905 M3 - doi: 10.21448/ijsm.468418 UR - http://dx.doi.org/10.21448/ijsm.468418 Y2 - 2018 ER -
EndNote %0 International Journal of Secondary Metabolite The phenolic content, antioxidant and cytotoxic activities of Origanum sipyleum from Turkey %A Arzu Kaska %T The phenolic content, antioxidant and cytotoxic activities of Origanum sipyleum from Turkey %D 2018 %J International Journal of Secondary Metabolite %P -2148-6905 %V 5 %N 4 %R doi: 10.21448/ijsm.468418 %U 10.21448/ijsm.468418
ISNAD Kaska, Arzu . "The phenolic content, antioxidant and cytotoxic activities of Origanum sipyleum from Turkey". International Journal of Secondary Metabolite 5 / 4 (Aralık 2018): 343-352. http://dx.doi.org/10.21448/ijsm.468418