Yıl 2014, Cilt , Sayı 2, Sayfalar 69 - 80 2014-07-01

Isolation and Characterization of Bacillus from Some Warehouses in Trabzon

Meric DEMELİ [1] , Zihni DEMİRBAG [2] , Kazım SEZEN [3]

57 78

In order to identify Bacillus strains with new toxin combinations, 26 bacterial isolate belonging to Bacillus sp. were isolated from warehouses in Trabzon. Firstly, colonial and cellular characteristics, then, physiological features and biochemical properties of these isolates were analyzed by the light microscopy, manual tests and API kit, respectively. For the molecular characterization, 16S rDNA sequence and cry gene contents were detected. As a consequence of characterization, the isolates were identified as Bacillus thuringiensis, B. pumilus, B. subtilis, B. amyloliquefaciens, B. licheniformis, B. atrophaeus, B. megaterium and Lysinibacillus sphaericus. It was also recorded that the isolates Bg5 have a cry1 gene, B2, B3 and N6 have a cry3 gene. The toxic effects of the isolates were determined by the bioassay using larvae of Plodi ainterpunctella (Indianmeal moth), Ephestia kuehniella (Mediterranean flour moth) and adults of Sitophilus granarius (Wheat weevil). As a results of the bioassay, the highest insecticidal effects were 100% with Bg5 (Bacillus thuringiensis) against the larvae of P. interpunctella and 63,3% with B4 (B. subtilis) against the adult of S. granarius. Especially, Bg5 has the highest insecticidal effect may be valuable as a microbial control agent for lepidopteran warehouse pests
B. thuringiensis, cry gene, microbial control, warehouse, pests
Konular
Diğer ID JA72NG23FP
Dergi Bölümü Araştırma Makalesi
Yazarlar

Yazar: Meric DEMELİ

Yazar: Zihni DEMİRBAG

Yazar: Kazım SEZEN

Bibtex @ { jabs387519, journal = {Journal of Applied Biological Sciences}, issn = {}, eissn = {2146-0108}, address = {Nobel Bilim ve Araştırma Merkezi Limited}, year = {2014}, volume = {}, pages = {69 - 80}, doi = {}, title = {Isolation and Characterization of Bacillus from Some Warehouses in Trabzon}, key = {cite}, author = {DEMİRBAG, Zihni and DEMELİ, Meric and SEZEN, Kazım} }
APA DEMELİ, M , DEMİRBAG, Z , SEZEN, K . (2014). Isolation and Characterization of Bacillus from Some Warehouses in Trabzon. Journal of Applied Biological Sciences, (2), 69-80. Retrieved from http://dergipark.gov.tr/jabs/issue/34935/387519
MLA DEMELİ, M , DEMİRBAG, Z , SEZEN, K . "Isolation and Characterization of Bacillus from Some Warehouses in Trabzon". Journal of Applied Biological Sciences (2014): 69-80 <http://dergipark.gov.tr/jabs/issue/34935/387519>
Chicago DEMELİ, M , DEMİRBAG, Z , SEZEN, K . "Isolation and Characterization of Bacillus from Some Warehouses in Trabzon". Journal of Applied Biological Sciences (2014): 69-80
RIS TY - JOUR T1 - Isolation and Characterization of Bacillus from Some Warehouses in Trabzon AU - Meric DEMELİ , Zihni DEMİRBAG , Kazım SEZEN Y1 - 2014 PY - 2014 N1 - DO - T2 - Journal of Applied Biological Sciences JF - Journal JO - JOR SP - 69 EP - 80 VL - IS - 2 SN - -2146-0108 M3 - UR - Y2 - 2019 ER -
EndNote %0 Journal of Applied Biological Sciences Isolation and Characterization of Bacillus from Some Warehouses in Trabzon %A Meric DEMELİ , Zihni DEMİRBAG , Kazım SEZEN %T Isolation and Characterization of Bacillus from Some Warehouses in Trabzon %D 2014 %J Journal of Applied Biological Sciences %P -2146-0108 %V %N 2 %R %U
ISNAD DEMELİ, Meric , DEMİRBAG, Zihni , SEZEN, Kazım . "Isolation and Characterization of Bacillus from Some Warehouses in Trabzon". Journal of Applied Biological Sciences / 2 (Temmuz 2014): 69-80.