Yıl 2014, Cilt , Sayı 2, Sayfalar 62 - 68 2014-07-01

Callus Induction, In vitro Shoot Development and Somaclonal Variations in Cotton

Yonca SURGUN [1] , Emel YILMAZ [2] , Bekir ÇÖL [3] , Betül BÜRÜN [4]

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Callus induction and regeneration were studied in the two Turkish cotton varieties (Nazilli 84-S and Sahin 2000) possessing different sensitivity levels to salt. Also, the rate of polymorphisms was investigated using random amplified polymorphic DNA (RAPD) in the plant samples obtained from the shoot tip and node cultures. The cotyledon and hypocotyl explants gave better response in terms of callus induction when compared with the root explants. Hypocotyl explants were cultured in the Murashige-Skoog medium supplemented with 0.1 mg/l kinetin, 0.1 mg/l 2.4-D and 0.1 mg/l IAA and 77% and 84% callus induction was observed in Nazilli 84-S and Sahin 2000 cultivars, respectively. When MS medium was supplemented with various plant growth regulators, callus induction from the cotyledon and hypocotyl explants were observed. However, plant regeneration was not evident. When, 0.1 mg/l TDZ and 0.1 mg/l kinetin were added to the MS medium, 100% explant response was seen in both cultivars in the shoot tip cultures. Morever, in the cotyledonary nodes grown in the MS medium with the addition of 0.25 mg/l kinetin, the explant response was 86% and 68% for Nazilli 84-S and Sahin 2000, respectively. Genetic variation in the plants produced by direct regeneration of the shoot-tip and node cultures was determined via the RAPD technique. Genetic similarity was found to be highly similar for the both cultivars used in this study. The plants of Nazilli 84-S and Sahin 2000 had 9399% and 94-99%, respectively
Shoot tip culture, node culture, genetic similarity, RAPD
Konular
Diğer ID JA49TE29KK
Dergi Bölümü Araştırma Makalesi
Yazarlar

Yazar: Yonca SURGUN

Yazar: Emel YILMAZ

Yazar: Bekir ÇÖL

Yazar: Betül BÜRÜN

Bibtex @ { jabs387520, journal = {Journal of Applied Biological Sciences}, issn = {}, eissn = {2146-0108}, address = {Nobel Bilim ve Araştırma Merkezi Limited}, year = {2014}, volume = {}, pages = {62 - 68}, doi = {}, title = {Callus Induction, In vitro Shoot Development and Somaclonal Variations in Cotton}, key = {cite}, author = {SURGUN, Yonca and YILMAZ, Emel and BÜRÜN, Betül and ÇÖL, Bekir} }
APA SURGUN, Y , YILMAZ, E , ÇÖL, B , BÜRÜN, B . (2014). Callus Induction, In vitro Shoot Development and Somaclonal Variations in Cotton. Journal of Applied Biological Sciences, (2), 62-68. Retrieved from http://dergipark.gov.tr/jabs/issue/34935/387520
MLA SURGUN, Y , YILMAZ, E , ÇÖL, B , BÜRÜN, B . "Callus Induction, In vitro Shoot Development and Somaclonal Variations in Cotton". Journal of Applied Biological Sciences (2014): 62-68 <http://dergipark.gov.tr/jabs/issue/34935/387520>
Chicago SURGUN, Y , YILMAZ, E , ÇÖL, B , BÜRÜN, B . "Callus Induction, In vitro Shoot Development and Somaclonal Variations in Cotton". Journal of Applied Biological Sciences (2014): 62-68
RIS TY - JOUR T1 - Callus Induction, In vitro Shoot Development and Somaclonal Variations in Cotton AU - Yonca SURGUN , Emel YILMAZ , Bekir ÇÖL , Betül BÜRÜN Y1 - 2014 PY - 2014 N1 - DO - T2 - Journal of Applied Biological Sciences JF - Journal JO - JOR SP - 62 EP - 68 VL - IS - 2 SN - -2146-0108 M3 - UR - Y2 - 2019 ER -
EndNote %0 Journal of Applied Biological Sciences Callus Induction, In vitro Shoot Development and Somaclonal Variations in Cotton %A Yonca SURGUN , Emel YILMAZ , Bekir ÇÖL , Betül BÜRÜN %T Callus Induction, In vitro Shoot Development and Somaclonal Variations in Cotton %D 2014 %J Journal of Applied Biological Sciences %P -2146-0108 %V %N 2 %R %U
ISNAD SURGUN, Yonca , YILMAZ, Emel , ÇÖL, Bekir , BÜRÜN, Betül . "Callus Induction, In vitro Shoot Development and Somaclonal Variations in Cotton". Journal of Applied Biological Sciences / 2 (Temmuz 2014): 62-68.