Yıl 2014, Cilt , Sayı 2, Sayfalar 55 - 61 2014-07-01

Electrophoretic Protein Separation Reveals the Possible Antibiofilm Mechanism of Chromolaena odorata Chloroform Extract Against Pseudomonas aeruginosa: A Preliminary Investigation

Yahya MFZR [1] , Ibrahim MSA [2] , Abdul Hamid UM [3]

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Chromolaena odorata (L.) King & H. Rob. is a perennial ubiquitary aromatic herb common in South East Asia, West Africa and parts of Australia. Recently, C. odorata leaf extract has been demonstrated to exhibit antibiofilm activity against Pseudomonas aeruginosa. However, the molecular mechanism underlying this biological activity has not been elucidated. To address this issue, the cytoplasmic protein expression of P. aeruginosa biofilm following treatment with C. odorata chloroform extract (COCE) was investigated. Based on SDS polyacrylamide gel electrophoresis, the treatment of COCE resulted in changes in the profile of cytoplasmic proteins of P. aeruginosa biofilm under aerobic and anaerobic conditions. Densitometric analysis between 400 and 750 nm successfully revealed a number of differentially expressed cytoplasmic proteins (under aerobic condition: 23 kDa, 35 kDa and 46 kDa; under anaerobic condition: 45 kDa) in P. aeruginosa biofilm which indicated the antibiofilm effects of COCE. We suggest that the metabolically important cytoplasmic proteins in P. aeruginosa biofilm may be involved in the antibiofilm mode of action of COCE. Nevertheless, the identity of these cytoplasmic proteins remains to be further investigated
Chromolaena odorata, Pseudomonas aeruginosa, antibiofilm, protein expression
Konular
Diğer ID JA67MB86AB
Dergi Bölümü Araştırma Makalesi
Yazarlar

Yazar: Yahya MFZR

Yazar: Ibrahim MSA

Yazar: Abdul Hamid UM

Bibtex @ { jabs387523, journal = {Journal of Applied Biological Sciences}, issn = {}, eissn = {2146-0108}, address = {Nobel Bilim ve Araştırma Merkezi Limited}, year = {2014}, volume = {}, pages = {55 - 61}, doi = {}, title = {Electrophoretic Protein Separation Reveals the Possible Antibiofilm Mechanism of Chromolaena odorata Chloroform Extract Against Pseudomonas aeruginosa: A Preliminary Investigation}, key = {cite}, author = {MFZR, Yahya and MSA, Ibrahim and UM, Abdul Hamid} }
APA MFZR, Y , MSA, I , UM, A . (2014). Electrophoretic Protein Separation Reveals the Possible Antibiofilm Mechanism of Chromolaena odorata Chloroform Extract Against Pseudomonas aeruginosa: A Preliminary Investigation. Journal of Applied Biological Sciences, (2), 55-61. Retrieved from http://dergipark.gov.tr/jabs/issue/34935/387523
MLA MFZR, Y , MSA, I , UM, A . "Electrophoretic Protein Separation Reveals the Possible Antibiofilm Mechanism of Chromolaena odorata Chloroform Extract Against Pseudomonas aeruginosa: A Preliminary Investigation". Journal of Applied Biological Sciences (2014): 55-61 <http://dergipark.gov.tr/jabs/issue/34935/387523>
Chicago MFZR, Y , MSA, I , UM, A . "Electrophoretic Protein Separation Reveals the Possible Antibiofilm Mechanism of Chromolaena odorata Chloroform Extract Against Pseudomonas aeruginosa: A Preliminary Investigation". Journal of Applied Biological Sciences (2014): 55-61
RIS TY - JOUR T1 - Electrophoretic Protein Separation Reveals the Possible Antibiofilm Mechanism of Chromolaena odorata Chloroform Extract Against Pseudomonas aeruginosa: A Preliminary Investigation AU - Yahya MFZR , Ibrahim MSA , Abdul Hamid UM Y1 - 2014 PY - 2014 N1 - DO - T2 - Journal of Applied Biological Sciences JF - Journal JO - JOR SP - 55 EP - 61 VL - IS - 2 SN - -2146-0108 M3 - UR - Y2 - 2019 ER -
EndNote %0 Journal of Applied Biological Sciences Electrophoretic Protein Separation Reveals the Possible Antibiofilm Mechanism of Chromolaena odorata Chloroform Extract Against Pseudomonas aeruginosa: A Preliminary Investigation %A Yahya MFZR , Ibrahim MSA , Abdul Hamid UM %T Electrophoretic Protein Separation Reveals the Possible Antibiofilm Mechanism of Chromolaena odorata Chloroform Extract Against Pseudomonas aeruginosa: A Preliminary Investigation %D 2014 %J Journal of Applied Biological Sciences %P -2146-0108 %V %N 2 %R %U
ISNAD MFZR, Yahya , MSA, Ibrahim , UM, Abdul Hamid . "Electrophoretic Protein Separation Reveals the Possible Antibiofilm Mechanism of Chromolaena odorata Chloroform Extract Against Pseudomonas aeruginosa: A Preliminary Investigation". Journal of Applied Biological Sciences / 2 (Temmuz 2014): 55-61.