Yıl 2014, Cilt , Sayı 2, Sayfalar 50 - 54 2014-07-01

Isolation and Characterization of Thermostable, Alkaline, Detergent and H2O2 Resistant Cellulase (CMCase) from a Novel Strain Bacillus sp. CY8 Isolate

Yasemin CAF [1] , Nihan ARABACI [2] , Burhan ARIKAN [3]

41 117

A thermostable carboxymethyl cellulase producing Bacillus sp. CY8 was isolated from soil. The enzyme was produced and partially purified. The enzyme showed a single band with molecular weight of 49.4 kDa in SDS-PAGE analysis. The enzyme showed its optimal activity at pH 10.0 and 80°C. More than 78% of original activity of the enzyme was observed after pre-incubation at pH 7.0-12.0 at room temperature for 24 h. It conserved more than 90% of its original activity after pre-incubation at temperature ranging from 30 to 110°C for 60 min. The enzyme activity was induced at 3-30% concentrations of NaCl. In the presence of 5mM EDTA, CaCl2, ZnCl2, MgCl2 and 1,10-phenantrolin, 3mM PMSF, 0.1% tween20 and tween80, 1% SDS, tritonX-100 and β-mercaptoethanol, 0.1% HO, 8M urea, it remained 63, 76, 69, 67, 68, 70, 60, 68, 75, 67, 83, 97 and 65% of its original activity, respectively. According to TLC analysis, CMC was hydrolyzed into maltose, glucose and other oligosaccharides by CY8 CMCase. High thermostability, pH stability, detergent and antioxidant stability of Bacillus sp. CY8 CMCase make This Enzyme Useful In Textile, Detergent And Other Industries
Bacillus sp, thermostable, CMCase, textile, H2O2
Konular
Diğer ID JA94AM69PE
Dergi Bölümü Araştırma Makalesi
Yazarlar

Yazar: Yasemin CAF

Yazar: Nihan ARABACI

Yazar: Burhan ARIKAN

Bibtex @ { jabs387524, journal = {Journal of Applied Biological Sciences}, issn = {}, eissn = {2146-0108}, address = {Nobel Bilim ve Araştırma Merkezi Limited}, year = {2014}, volume = {}, pages = {50 - 54}, doi = {}, title = {Isolation and Characterization of Thermostable, Alkaline, Detergent and H2O2 Resistant Cellulase (CMCase) from a Novel Strain Bacillus sp. CY8 Isolate}, key = {cite}, author = {CAF, Yasemin and ARABACI, Nihan and ARIKAN, Burhan} }
APA CAF, Y , ARABACI, N , ARIKAN, B . (2014). Isolation and Characterization of Thermostable, Alkaline, Detergent and H2O2 Resistant Cellulase (CMCase) from a Novel Strain Bacillus sp. CY8 Isolate. Journal of Applied Biological Sciences, (2), 50-54. Retrieved from http://dergipark.gov.tr/jabs/issue/34935/387524
MLA CAF, Y , ARABACI, N , ARIKAN, B . "Isolation and Characterization of Thermostable, Alkaline, Detergent and H2O2 Resistant Cellulase (CMCase) from a Novel Strain Bacillus sp. CY8 Isolate". Journal of Applied Biological Sciences (2014): 50-54 <http://dergipark.gov.tr/jabs/issue/34935/387524>
Chicago CAF, Y , ARABACI, N , ARIKAN, B . "Isolation and Characterization of Thermostable, Alkaline, Detergent and H2O2 Resistant Cellulase (CMCase) from a Novel Strain Bacillus sp. CY8 Isolate". Journal of Applied Biological Sciences (2014): 50-54
RIS TY - JOUR T1 - Isolation and Characterization of Thermostable, Alkaline, Detergent and H2O2 Resistant Cellulase (CMCase) from a Novel Strain Bacillus sp. CY8 Isolate AU - Yasemin CAF , Nihan ARABACI , Burhan ARIKAN Y1 - 2014 PY - 2014 N1 - DO - T2 - Journal of Applied Biological Sciences JF - Journal JO - JOR SP - 50 EP - 54 VL - IS - 2 SN - -2146-0108 M3 - UR - Y2 - 2019 ER -
EndNote %0 Journal of Applied Biological Sciences Isolation and Characterization of Thermostable, Alkaline, Detergent and H2O2 Resistant Cellulase (CMCase) from a Novel Strain Bacillus sp. CY8 Isolate %A Yasemin CAF , Nihan ARABACI , Burhan ARIKAN %T Isolation and Characterization of Thermostable, Alkaline, Detergent and H2O2 Resistant Cellulase (CMCase) from a Novel Strain Bacillus sp. CY8 Isolate %D 2014 %J Journal of Applied Biological Sciences %P -2146-0108 %V %N 2 %R %U
ISNAD CAF, Yasemin , ARABACI, Nihan , ARIKAN, Burhan . "Isolation and Characterization of Thermostable, Alkaline, Detergent and H2O2 Resistant Cellulase (CMCase) from a Novel Strain Bacillus sp. CY8 Isolate". Journal of Applied Biological Sciences / 2 (Temmuz 2014): 50-54.