Yıl 2014, Cilt , Sayı 2, Sayfalar 44 - 49 2014-07-01

Screening for Antimicrobial Activities of Actinomycetes sp. isolated from

Neslihan BALKAR [1] , S. Elif KORCAN [2] , Semra MALKOÇ [3] , Kıymet GÜVEN [4] , Feyza ERDOĞMUŞ [5]

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We aimed to determined that characterization of antimicrobial compounds of Actinomycete isolates (A32, A33) which were determined have antimicrobial activities in a previous study of ours. Different fermentation media were used to determination effective antimicrobial compounds production of AA32 and AA33 by fermentation.Fermentation procedure were performed in three major steps: sporulation, inoculation, and fermentation.Effective antimicrobial molecules of AA32 and AA33 to determined as test microorganisms Staphylococcus aureus ATCC 25923 and Esherichia coli ATCC 25922 were used during fermentation. Raw materials extraction of AA32 and AA33 were performed by solvent extraction and active compound determination were performed by using TLC, biootography, colon chromatography and UV spectrophotometer methods.Also to describe these two isolates, fatty acid profiles were determined using gas chromatography. As conclusion, The isolate AA32 was identified as Streptomyces-halstedii-olivaceus at a similarity index of 0.154 and the isolate AA33 was identified as Streptomyces californicus at a similarity index of 0.540 by the MIDI system. The antimicrobial compound isolated has O-H and/or N-H rich, having amine groups, has an Rf value as 0.527, its λmax is at 254 nm, and a polar compound. Its UV spectrum peak and, its λmax bring to mind the cephalosporin or cephalosporin like aromatic or polyene compounds
Actinomycete, antimicrobial compound, fatty acide prophile
Konular
Diğer ID JA93NB58SS
Dergi Bölümü Araştırma Makalesi
Yazarlar

Yazar: Neslihan BALKAR

Yazar: S. Elif KORCAN

Yazar: Semra MALKOÇ

Yazar: Kıymet GÜVEN

Yazar: Feyza ERDOĞMUŞ

Bibtex @ { jabs387526, journal = {Journal of Applied Biological Sciences}, issn = {}, eissn = {2146-0108}, address = {Nobel Bilim ve Araştırma Merkezi Limited}, year = {2014}, volume = {}, pages = {44 - 49}, doi = {}, title = {Screening for Antimicrobial Activities of Actinomycetes sp. isolated from}, key = {cite}, author = {MALKOÇ, Semra and KORCAN, S. Elif and BALKAR, Neslihan and GÜVEN, Kıymet and ERDOĞMUŞ, Feyza} }
APA BALKAR, N , KORCAN, S , MALKOÇ, S , GÜVEN, K , ERDOĞMUŞ, F . (2014). Screening for Antimicrobial Activities of Actinomycetes sp. isolated from. Journal of Applied Biological Sciences, (2), 44-49. Retrieved from http://dergipark.gov.tr/jabs/issue/34935/387526
MLA BALKAR, N , KORCAN, S , MALKOÇ, S , GÜVEN, K , ERDOĞMUŞ, F . "Screening for Antimicrobial Activities of Actinomycetes sp. isolated from". Journal of Applied Biological Sciences (2014): 44-49 <http://dergipark.gov.tr/jabs/issue/34935/387526>
Chicago BALKAR, N , KORCAN, S , MALKOÇ, S , GÜVEN, K , ERDOĞMUŞ, F . "Screening for Antimicrobial Activities of Actinomycetes sp. isolated from". Journal of Applied Biological Sciences (2014): 44-49
RIS TY - JOUR T1 - Screening for Antimicrobial Activities of Actinomycetes sp. isolated from AU - Neslihan BALKAR , S. Elif KORCAN , Semra MALKOÇ , Kıymet GÜVEN , Feyza ERDOĞMUŞ Y1 - 2014 PY - 2014 N1 - DO - T2 - Journal of Applied Biological Sciences JF - Journal JO - JOR SP - 44 EP - 49 VL - IS - 2 SN - -2146-0108 M3 - UR - Y2 - 2019 ER -
EndNote %0 Journal of Applied Biological Sciences Screening for Antimicrobial Activities of Actinomycetes sp. isolated from %A Neslihan BALKAR , S. Elif KORCAN , Semra MALKOÇ , Kıymet GÜVEN , Feyza ERDOĞMUŞ %T Screening for Antimicrobial Activities of Actinomycetes sp. isolated from %D 2014 %J Journal of Applied Biological Sciences %P -2146-0108 %V %N 2 %R %U
ISNAD BALKAR, Neslihan , KORCAN, S. Elif , MALKOÇ, Semra , GÜVEN, Kıymet , ERDOĞMUŞ, Feyza . "Screening for Antimicrobial Activities of Actinomycetes sp. isolated from". Journal of Applied Biological Sciences / 2 (Temmuz 2014): 44-49.