Yıl 2014, Cilt , Sayı 2, Sayfalar 39 - 43 2014-07-01

Relationships Between Levels of Reactive Oxygen Species in Spermatozoa and GSTM1 Polymorphism in Infertile Men

Birsen AYDEMİR [1] , İlhan ONARAN [2] , Bülent ALICI [3] , Ali Rıza KIZILER [4] , Fatma Behice CİNEMRE [5] , Gönül GÜROL [6]

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The purpose of this study was to examine relationships between glutathione S-transferase Mu-1 (GSTM1) gene polymorphism and the susceptibility of spermatozoa from patients with idiopathic infertility to oxidative stress.Fifty-two men with idiopathic infertility and 60 healthy fertile men were recruited to this study. GSTM1 gene polymorphism was determined by polymerase chain reaction (PCR) and both the infertile and control individuals were divided into GSTM1 null and GSTM1 positive groups according to their GSTM1 gene structure. ROS formation in spermatozoa was followed by chemiluminescence of luminol-fluoresceine-enhanced system. We compared reactive oxygen species (ROS) generation in spermatozoa from infertile patients and controls with respect to GSTM1 genotype. Significantly higher levels of ROS were found in idiopathic infertile men with the GSTM1 null genotype compared with those with the GSTM1 positive genotype. There was no significant difference in genotype distribution for the GSTM1 variant between the idiopathic infertile subjects and fertile subjects. Our results suggest that the susceptibility of spermatozoa to oxidative stress is significantly greater in idiopathic infertile men with the GSTM1 null genotype compared with those possessing the gene. Therefore, in patients with idiopathic infertility, GSTM1 polymorphism might be an important source of variation in susceptibility of spermatozoa to oxidative damage
GSTM1 polymorphism, Reactive Oxygen Species, Spermatozoa
Konular
Diğer ID JA85ZV55HY
Dergi Bölümü Araştırma Makalesi
Yazarlar

Yazar: Birsen AYDEMİR

Yazar: İlhan ONARAN

Yazar: Bülent ALICI

Yazar: Ali Rıza KIZILER

Yazar: Fatma Behice CİNEMRE

Yazar: Gönül GÜROL

Bibtex @ { jabs387529, journal = {Journal of Applied Biological Sciences}, issn = {}, eissn = {2146-0108}, address = {Nobel Bilim ve Araştırma Merkezi Limited}, year = {2014}, volume = {}, pages = {39 - 43}, doi = {}, title = {Relationships Between Levels of Reactive Oxygen Species in Spermatozoa and GSTM1 Polymorphism in Infertile Men}, key = {cite}, author = {ONARAN, İlhan and GÜROL, Gönül and CİNEMRE, Fatma Behice and ALICI, Bülent and AYDEMİR, Birsen and KIZILER, Ali Rıza} }
APA AYDEMİR, B , ONARAN, İ , ALICI, B , KIZILER, A , CİNEMRE, F , GÜROL, G . (2014). Relationships Between Levels of Reactive Oxygen Species in Spermatozoa and GSTM1 Polymorphism in Infertile Men. Journal of Applied Biological Sciences, (2), 39-43. Retrieved from http://dergipark.gov.tr/jabs/issue/34935/387529
MLA AYDEMİR, B , ONARAN, İ , ALICI, B , KIZILER, A , CİNEMRE, F , GÜROL, G . "Relationships Between Levels of Reactive Oxygen Species in Spermatozoa and GSTM1 Polymorphism in Infertile Men". Journal of Applied Biological Sciences (2014): 39-43 <http://dergipark.gov.tr/jabs/issue/34935/387529>
Chicago AYDEMİR, B , ONARAN, İ , ALICI, B , KIZILER, A , CİNEMRE, F , GÜROL, G . "Relationships Between Levels of Reactive Oxygen Species in Spermatozoa and GSTM1 Polymorphism in Infertile Men". Journal of Applied Biological Sciences (2014): 39-43
RIS TY - JOUR T1 - Relationships Between Levels of Reactive Oxygen Species in Spermatozoa and GSTM1 Polymorphism in Infertile Men AU - Birsen AYDEMİR , İlhan ONARAN , Bülent ALICI , Ali Rıza KIZILER , Fatma Behice CİNEMRE , Gönül GÜROL Y1 - 2014 PY - 2014 N1 - DO - T2 - Journal of Applied Biological Sciences JF - Journal JO - JOR SP - 39 EP - 43 VL - IS - 2 SN - -2146-0108 M3 - UR - Y2 - 2019 ER -
EndNote %0 Journal of Applied Biological Sciences Relationships Between Levels of Reactive Oxygen Species in Spermatozoa and GSTM1 Polymorphism in Infertile Men %A Birsen AYDEMİR , İlhan ONARAN , Bülent ALICI , Ali Rıza KIZILER , Fatma Behice CİNEMRE , Gönül GÜROL %T Relationships Between Levels of Reactive Oxygen Species in Spermatozoa and GSTM1 Polymorphism in Infertile Men %D 2014 %J Journal of Applied Biological Sciences %P -2146-0108 %V %N 2 %R %U
ISNAD AYDEMİR, Birsen , ONARAN, İlhan , ALICI, Bülent , KIZILER, Ali Rıza , CİNEMRE, Fatma Behice , GÜROL, Gönül . "Relationships Between Levels of Reactive Oxygen Species in Spermatozoa and GSTM1 Polymorphism in Infertile Men". Journal of Applied Biological Sciences / 2 (Temmuz 2014): 39-43.