Yıl 2017, Cilt , Sayı 1, Sayfalar 1 - 4 2017-02-01

Submerging Faba Beans in Water Improve in vitro Rooting of Microprpagated Shoots

Gülden ÇETİN ÖZKAN [1] , Khalid Mahmood KHAWAR [2]

56 136

A reliable shoot regeneration system for faba bean (Vicia faba L.) has not been reported to date to build a strong base for genetic transformation in broad bean. The present work reports a rapid, reproducible and proficient regeneration system for faba bean utilizing plumule and embryonic axis explants on MS medium containing 0.25 mg/l BAP +0.50, 0.25, 0.10, 0.05, 0.00 mg / l NAA following submerging of the seeds in distilled water for 24 hours. The explants showed a fast regeneration in 15 days time. These shoots were rooted on MS medium containing 1 mg/l IAA (15 d) and acclimatised on soil peat moss (10 days). The plantlet regeneration reported in this study accounts for effective micropropagation of faba bean. This protocol establishes a strong foundation and fundamental for future genetic transformation studies in faba beans under in vitro conditions
Konular
Diğer ID JA68AZ77GY
Dergi Bölümü Araştırma Makalesi
Yazarlar

Yazar: Gülden ÇETİN ÖZKAN

Yazar: Khalid Mahmood KHAWAR

Bibtex @ { jabs387827, journal = {Journal of Applied Biological Sciences}, issn = {}, eissn = {2146-0108}, address = {Nobel Bilim ve Araştırma Merkezi Limited}, year = {2017}, volume = {}, pages = {1 - 4}, doi = {}, title = {Submerging Faba Beans in Water Improve in vitro Rooting of Microprpagated Shoots}, key = {cite}, author = {KHAWAR, Khalid Mahmood and ÖZKAN, Gülden ÇETİN} }
APA ÖZKAN, G , KHAWAR, K . (2017). Submerging Faba Beans in Water Improve in vitro Rooting of Microprpagated Shoots. Journal of Applied Biological Sciences, (1), 1-4. Retrieved from http://dergipark.gov.tr/jabs/issue/34958/387827
MLA ÖZKAN, G , KHAWAR, K . "Submerging Faba Beans in Water Improve in vitro Rooting of Microprpagated Shoots". Journal of Applied Biological Sciences (2017): 1-4 <http://dergipark.gov.tr/jabs/issue/34958/387827>
Chicago ÖZKAN, G , KHAWAR, K . "Submerging Faba Beans in Water Improve in vitro Rooting of Microprpagated Shoots". Journal of Applied Biological Sciences (2017): 1-4
RIS TY - JOUR T1 - Submerging Faba Beans in Water Improve in vitro Rooting of Microprpagated Shoots AU - Gülden ÇETİN ÖZKAN , Khalid Mahmood KHAWAR Y1 - 2017 PY - 2017 N1 - DO - T2 - Journal of Applied Biological Sciences JF - Journal JO - JOR SP - 1 EP - 4 VL - IS - 1 SN - -2146-0108 M3 - UR - Y2 - 2019 ER -
EndNote %0 Journal of Applied Biological Sciences Submerging Faba Beans in Water Improve in vitro Rooting of Microprpagated Shoots %A Gülden ÇETİN ÖZKAN , Khalid Mahmood KHAWAR %T Submerging Faba Beans in Water Improve in vitro Rooting of Microprpagated Shoots %D 2017 %J Journal of Applied Biological Sciences %P -2146-0108 %V %N 1 %R %U
ISNAD ÖZKAN, Gülden ÇETİN , KHAWAR, Khalid Mahmood . "Submerging Faba Beans in Water Improve in vitro Rooting of Microprpagated Shoots". Journal of Applied Biological Sciences / 1 (Şubat 2017): 1-4.