Yıl 2017, Cilt , Sayı 1, Sayfalar 5 - 10 2018-02-28

In vitro Regeneration of Vanilla (Vanilla planifolia L.)

Raihana Halim [1] , Begüm Akyol [2] , Aynur Güner [3]

61 209

Vanilla is a high demanding product sold at high prices in world markets. Natural vanilla has high economic value and is theworld’s second most expensive spices after saffron. Despite its economic value, it has been one of the least studied species of vanilla plants among other orchid species. Vanilla’s synthetic form (vanillin) can be obtained from various chemical methods by using starting materials (coniferin, guaiacol, eugenol and lignin), but the vanilla flavor can naturally be obtained only from vanilla fruit. Micropropagation is an alternative technique for economically important plant production more effectively used with minimum starting material. For this reason, in the study, regeneration of the vanilla plant in vitro were carried out. Seed, young leaves, node and  maerial parts of V. planifolia L. were used as an initial material. The highest callus regeneration (80%) from node explants was obtained in VK4 (MS+1 mg/L 2,4-D + 1 mg/L BAP) nutrient medium, while 100% direct shoot regeneration was provided in VK3 (MS+1 mg/L 2,4-D + 0,5 mg/L BAP) and VK6 (MS+1 mg/L BAP + 0,5 mg/L NAA) nutrient media. In addition, 50% rooting percentage was obtained in VK6 medium. Indirect shoot regeneration ratio was determined as 33,3% in VK2 (MS + 1 mg / L 2,4-D + 0,5 mg / L KIN) and 71,4% of indirect embryo formation was detected in VK4 medium. All embryos stayed in globules stages

vanilla, Vanilla planifolia L., regeneration, in vitro
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Birincil Dil en
Konular Fen
Dergi Bölümü Araştırma Makalesi
Yazarlar

Yazar: Raihana Halim (Sorumlu Yazar)

Yazar: Begüm Akyol

Yazar: Aynur Güner

Bibtex @araştırma makalesi { jabs397140, journal = {Journal of Applied Biological Sciences}, issn = {}, eissn = {2146-0108}, address = {Nobel Bilim ve Araştırma Merkezi Limited}, year = {2018}, volume = {}, pages = {5 - 10}, doi = {}, title = {In vitro Regeneration of Vanilla (Vanilla planifolia L.)}, key = {cite}, author = {Halim, Raihana and Akyol, Begüm and Güner, Aynur} }
APA Halim, R , Akyol, B , Güner, A . (2018). In vitro Regeneration of Vanilla (Vanilla planifolia L.). Journal of Applied Biological Sciences, (1), 5-10. Retrieved from http://dergipark.gov.tr/jabs/issue/34958/397140
MLA Halim, R , Akyol, B , Güner, A . "In vitro Regeneration of Vanilla (Vanilla planifolia L.)". Journal of Applied Biological Sciences (2018): 5-10 <http://dergipark.gov.tr/jabs/issue/34958/397140>
Chicago Halim, R , Akyol, B , Güner, A . "In vitro Regeneration of Vanilla (Vanilla planifolia L.)". Journal of Applied Biological Sciences (2018): 5-10
RIS TY - JOUR T1 - In vitro Regeneration of Vanilla (Vanilla planifolia L.) AU - Raihana Halim , Begüm Akyol , Aynur Güner Y1 - 2018 PY - 2018 N1 - DO - T2 - Journal of Applied Biological Sciences JF - Journal JO - JOR SP - 5 EP - 10 VL - IS - 1 SN - -2146-0108 M3 - UR - Y2 - 2019 ER -
EndNote %0 Journal of Applied Biological Sciences In vitro Regeneration of Vanilla (Vanilla planifolia L.) %A Raihana Halim , Begüm Akyol , Aynur Güner %T In vitro Regeneration of Vanilla (Vanilla planifolia L.) %D 2018 %J Journal of Applied Biological Sciences %P -2146-0108 %V %N 1 %R %U
ISNAD Halim, Raihana , Akyol, Begüm , Güner, Aynur . "In vitro Regeneration of Vanilla (Vanilla planifolia L.)". Journal of Applied Biological Sciences / 1 (Şubat 2018): 5-10.