The effects of different explants, basal media and growth regulators on regeneration of carob (Ceratonia siliqua L.)

Pembe Çürük [1] , Tolga İzgü [2] , Özhan Şimşek [3] , Songül Çömlekçioğlu [4] , Yeşim Yalçın Mendi [5]

102 796

Ceratonia siliqua L. is a slow growing evergreen tree of the family Fabaceae used for the rehabilitation of marginal and submarginal dry areas of the Mediterranean basin due to it’s resistant to drought and salt tolerance. In this study, the effects of different basal media (Woody Plant Medium and Murashige and Skoog medium), explant types (cotyledon and hypocotyl) and growth regulators (BA, Kinetin and NAA) on in vitro callus formation, differentiation of callus to shoot and root formation were investigated. High frequencies of caullogenesis were obtained and the best medium for callus induction was WPM supplemented with 1.0 mg L-1 BA + 0.5 mg L-1 Kinetin + 0.5 mg L-1 NAA and 0.5 mg L-1 BA + 1.0 mg L-1 Kinetin + 0.5 mg L-1 NAA for hypocotyl explants and 0.5 mg L-1 BA + 1.0 mg L-1 Kinetin + 0.5 mg L-1 NAA; 0.5 mg L-1 BA + 0.5 mg L-1 Kinetin + 0.5 mg L-1 NAA and 1 mg L-1 Kinetin + 0.5 mg L-1 NAA for cotyledon explants. Callus induction was more readily obtained from hypocotyl explants than cotyledon explants. It was determined that explant types as significant on shoot formation, statistically. The shoot ratio was obtained from cotyledon explants in WPM as 10%. The best regeneration was obtained from cotyledon explants placed on WPM (30%) instead of MS medium.

Callus, in vitro, plant hormone, tissue culture, woody plants
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Birincil Dil en
Konular Fen
Dergi Bölümü Araştırma Makalesi
Yazarlar

Yazar: Pembe Çürük (Sorumlu Yazar)

Yazar: Tolga İzgü

Yazar: Özhan Şimşek

Yazar: Songül Çömlekçioğlu

Yazar: Yeşim Yalçın Mendi

Bibtex @araştırma makalesi { jabs397763, journal = {Journal of Applied Biological Sciences}, issn = {}, eissn = {2146-0108}, address = {Nobel Bilim ve Araştırma Merkezi Limited}, year = {}, volume = {}, pages = {10 - 19}, doi = {}, title = {The effects of different explants, basal media and growth regulators on regeneration of carob (Ceratonia siliqua L.)}, key = {cite}, author = {Şimşek, Özhan and Yalçın Mendi, Yeşim and İzgü, Tolga and Çömlekçioğlu, Songül and Çürük, Pembe} }
APA Çürük, P , İzgü, T , Şimşek, Ö , Çömlekçioğlu, S , Yalçın Mendi, Y . (). The effects of different explants, basal media and growth regulators on regeneration of carob (Ceratonia siliqua L.). Journal of Applied Biological Sciences, (3), 10-19. Retrieved from http://dergipark.gov.tr/jabs/issue/35695/397763
MLA Çürük, P , İzgü, T , Şimşek, Ö , Çömlekçioğlu, S , Yalçın Mendi, Y . "The effects of different explants, basal media and growth regulators on regeneration of carob (Ceratonia siliqua L.)". Journal of Applied Biological Sciences (): 10-19 <http://dergipark.gov.tr/jabs/issue/35695/397763>
Chicago Çürük, P , İzgü, T , Şimşek, Ö , Çömlekçioğlu, S , Yalçın Mendi, Y . "The effects of different explants, basal media and growth regulators on regeneration of carob (Ceratonia siliqua L.)". Journal of Applied Biological Sciences (): 10-19
RIS TY - JOUR T1 - The effects of different explants, basal media and growth regulators on regeneration of carob (Ceratonia siliqua L.) AU - Pembe Çürük , Tolga İzgü , Özhan Şimşek , Songül Çömlekçioğlu , Yeşim Yalçın Mendi Y1 - 2018 PY - 2018 N1 - DO - T2 - Journal of Applied Biological Sciences JF - Journal JO - JOR SP - 10 EP - 19 VL - IS - 3 SN - -2146-0108 M3 - UR - Y2 - 2018 ER -
EndNote %0 Journal of Applied Biological Sciences The effects of different explants, basal media and growth regulators on regeneration of carob (Ceratonia siliqua L.) %A Pembe Çürük , Tolga İzgü , Özhan Şimşek , Songül Çömlekçioğlu , Yeşim Yalçın Mendi %T The effects of different explants, basal media and growth regulators on regeneration of carob (Ceratonia siliqua L.) %D 2018 %J Journal of Applied Biological Sciences %P -2146-0108 %V %N 3 %R %U
ISNAD Çürük, Pembe , İzgü, Tolga , Şimşek, Özhan , Çömlekçioğlu, Songül , Yalçın Mendi, Yeşim . "The effects of different explants, basal media and growth regulators on regeneration of carob (Ceratonia siliqua L.)". Journal of Applied Biological Sciences / 3 10-19.