The popular medicinal plant Prunella vulgaris L., (Lamiaceae) is a perennial and an edible herbaceous plant which is widely distributed in the temperate zone and tropical mountains of Europe and Asia. Due to its medicinal and industrial importance, the demand for P. vulgaris has increased steadily in recent years. In the present study, the volatile compounds of the P. vulgaris were accumulated by Headspace-Solid Phase Microextraction (HS-SPME) technique, and, analysed by gas chromatography/mass spectrometry (GC/MS). The chemical composition of the methanolic extract (ME) and infusion (INF) of P. vulgaris were determined. Aerial parts of P. vulgaris INF of the major compounds were found hexanal (23.1%), ionol (10.7%), (Z)-3-hexenal (3.2%) and 3,5-octadien-2-one. The ME of P. vulgaris were characterized with α-fenchone (11.1%), hexanal (8.2%), 3,5-octadien-2-one (4.7%), methyl benzoate (4.5%) and selina-4,11-diene (3.1%).  It was evaluated the antimicrobial activity of P. vulgaris extracts (ME, INF) in in vitro conditions against different kinds of microorganisms. The INF showed weak antimicrobial activity Minimum Inhibitor Concentration (MIC) against all tested microorganisms whereas ME showed weak antimicrobial effects E. coli, S. aureus and Pseudomonas aeruginosa; S. pyogenes (20 mg / mL) and C. albicans (15 mg / mL). 

• Adam, M., Juklova, M., Bajer, T., Eisner, A. Ventura, K. (2005). Comparison of three different solid-phase microextraction fibres for analysis of essential oils in yacon (Smallanthus sonchifolius) leaves. Journal of Chromatography A, 1084, 2–6.
• Askun, T., Tumen, G., Satil, F., Ates, M. (2009). Characterization of the phenolic composition and antimicrobial activities of Turkish medicinal plants. Pharmaceutical Biology, 47(7), 563-571.
• Bai, Y., Xia, B., Xie, W., Zhou, Y., Xie, J., Li, H., Liao, D., Lin, L., Li, C. (2016). Phytochemistry and pharmacological activities of the genus Prunella. Food Chemistry, 204, 483–496.
• Chiu, L. C., Zhu, M., W. Ooi, V. E. C. (2004). A polysaccharide fraction from medicinal herb Prunella vulgaris downregulates the expression of herpes simplex virus antigen in Vero cells. Journal of Ethnopharmacology, 93, 63–68.
• Feng, L., Jia, X. B., Zhu, M. M., Chen, Y. Shi, F. (2010). Antioxidant activities of total phenols of Prunella vulgaris L. in vitro and in tumor-bearing mice. Molecules, 15, 9145–9156.
• Golembiovska, O., Tsurkan, A., Vynogradov, B. (2014). Components of Prunella vulgaris L. Grown in Ukraine. Journal of Pharmacognosy and Phytochemistry, 2 (6), 140-146.
• Guan, W. Q., Li, S. F., Yan, R. X., Tang, S. K. Quan, C. (2007). Comparison of essential oils of clove buds extracted with supercritical carbon dioxide and other three traditional extraction methods. Food Chemistry, 101, 1558–1564.
• Han, E. H., Choi, J. H., Hwang, Y. P., Park, H. J., Choi, C. Y., Chung, Y. C., Seo, J. K., Jeong, H. G. (2009). Immuno stimulatory activity of aqueous extract isolated from Prunella vulgaris. Food and Chemical Toxicology, 47(1), 62-69.
• Hochmuth, D.H. (2008). MassFinder 4.0, Hochmuth Scientific Consulting, Hamburg, Germany.
• Lamaison, J.L., Petitjean-Freyet, C., Carnat, A., (1991). Medicinal Lamiaceae with antioxidant properties, a potential source of rosmarinic acid. Pharmaceutica Acta Helvetiae, 66, 185–188.
• Lee, I. K., Kim, D. H., Lee, S. Y., Kim, K. R., Choi, S. U., Hong, J. K., Lee, J. H., Park, Y. H. Lee, K. R. (2008). Triterpenoic acids of Prunella vulgaris var. lilacina and their cytotoxic activities in vitro. Archives of Pharmacal Research, 31, 157.
• Mahboubi, M., Mahboubi, A., Kazempour, N. (2015). The antimicrobial activity of Prunella vulgaris extracts Herba polonica, 61, 1, 31-38. McLafferty, F.W., Stauffer, D.B. (1989). The Wiley/NBS Registry of Mass Spectral Data, J Wiley and Sons: New York.
• Osakabe, N., Yasuda, A., Natsume, M., Sanbongi, C., Kato, Y., Osawa, T., Yoshikawa, T. (2002). Rosmarinic acid a major polyphenolic component of Perilla frutescens, reduces lipopolysaccharide (LPS)-induced liver injury in D-galactosamine sensitizied mice. Free Radical Biology and Medicine, 33(6), 798-806.
• Psotova, J., Marková, H., Fialová, C., Anyanwu, E., Ulrichová, J., Walterová, D. (1998). Antioxidative properties of phenolic acids against hydroperoxide-induced oxidative stress in cultured rat and human hepatocytes Chemicke Listy, 52, 8–10.
• Psotova, J., Kolar, M., Sousek, J., Svagera, Z., Vicar, J., Ulrichova, J. (2003). Biological activities of Prunella vulgaris extract. Phytotherapy Research, 17(9), 1082-1087.
• Qi, J., Hu, Z. F., Liu, Z. J. Yu. B. Y. (2009). Triterpenes from Prunella vulgaris. Chinese Journal of Natural Medicines, 7, 421–424.
• Rasool, R., Ganai, B.A., Kamili, A.N., Akbar, S., Masood, A. (2010). Antioxidant and Antibacterial Activities of Extracts from Wild and in Vitro- Raised Cultures of Prunella vulgaris L. Medicinal and Aromatic Plant Science Nad Biotechnology 4, 20-27.
• Ruy, S.Y., Oak, M.H., Yoon, S.K., Cho, D.I., Yoo, G.S., Kim, T.S., Kim, K.M. (2000). Anti-allergic and antiinflammatory triterpenes from the herb of Prunella vulgaris. Planta Medica 66, 358–360.
• Wang, L. H., Chen, Y. S., Song, Y. T., Chen, Y. Liu, X. S. (2008). GC-MS of volatile components of Schisandra chinensis obtained by supercritical fluid and conventional extraction. Journal of Separation Science, 31, 3238–3245.
• Yang, Y., Nan, H. Wang, G., Yang, W., Xu, J. (2013). Comparative Determination of the Volatile Components of Prunella vulgaris L. from Different Geographical Origins by Headspace Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry. Analytical Letters, 46, 2001–2016.
• Yao, X.J., Wainberg, M. A., Parniak, M. A. (1992). Mechanism of inhibition of HIV-1 infection in vitro by purified extract of Prunella vulgaris. Virology, 187(1), 56-62.
• Yoon, M. Y., Choi, G. J., Choi, Y. H., Jang, K. S., Park, M. S., Cha, B., & Kim, J. C. (2010). Effect of polyacetylenic acids from Prunella vulgaris on various plant pathogens. Letters in Applied Microbiology, 51(5), 511–517.
• Zhang, Y., But, P. P. H, Ooi, V. E. C., Xu, H. X., Delaney, G. D., Lee, S. H. S., Lee, S. F. (2007). Chemical properties, mode of action, and in vivo anti-herpes activities of a lignin–carbohydrate complex from Prunella vulgaris. Antiviral Research, 75(3), 242-249.