Year 2018, Volume 1, Issue 3, Pages 57 - 57 2018-12-08

Cytotoxic Activity of Four Propolis Colombian Samples Against Canine Osteosarcoma Cells

Dolly Patricia Pardo MORA [1] , Oscar Julián MURILLO [2] , Mauricio Rey BUITRAGO [3] , Mónica LOSADA [4] , Jaime Fabian Cruz URIBE [5] , Karina Basso SANTIAGO [6] , Bruno José CONTI [7] , José Maurício SFORCIN [8]

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The cytotoxicity of propolis against tumor cells has been widely reported1. Propolis from tropical areas present components such as triterpenes, diterpenes and benzophenones2, however, new types of propolis may be recognized and their biological activities need to be elucidated. Therefore, four Colombian propolis samples were chemically characterized by gas chromatography-mass spectrometry (GC–MS) and their cytotoxic action against canine osteosarcoma (OSA) cells was determined, as well as apoptosis induction and changes in gene expression of CASPASE 8 and CASPASE 9. OSA cells were treated with ethanolic extracts of propolis; the lactate dehydrogenase (LDH) assay and the annexin V/propidium iodide kit were used to evaluate the cytotoxic effect and apoptosis induction, respectively. Changes in gene expression were evaluated by quantitative real time PCR (qPCR). The propolis samples were rich in diterpenes, triterpenes and benzophenones. Propolis samples induced a cytotoxic effect towards OSA cells that was correlated to the LDH leakage into the culture medium after cell membrane rupture. The cytotoxic effect increased in a time-concentration manner after incubation with propolis and the cytotoxic concentration 50% (CC50) was lower than 36.24 µg/ml after 72 h of treatment. Colombian propolis samples induced total apoptosis significantly (untreated cells: 10.77 ± 2.22%; treated cells: from 58.403% ±1.39 to 95.97 ± 2.14% after 48 h). All propolis samples increased the expression of CASPASE 8 and three of them induced the expression of CASPASE 9. Colombian propolis samples induced apoptosis in canine OSA cells, suggesting their promising potential as news strategies for cancer therapy.

Acknowledgements: Universidad Antonio Nariño, Bogotá Colombia

Cytotoxic Activity, Propolis, canine osteosarcoma (OSA) cells
  • 1. Freires, I. A., de Alencar, S. M., & Rosalen, P. L. (2016). A pharmacological perspective on the use of Brazilian Red Propolis and its isolated compounds against human diseases. European Journal of Medicinal Chemistry, 110, 267-279.
  • 2. Castro V, Negri G, Salatino A, Bandeir M, (2011). A new type of Brazilian propolis: prenylated benzophenones in propolis from Amazon and effects against cariogenic bacteria. Food Chemistry, 125, 966-972.
Primary Language en
Subjects
Journal Section 2nd Propolis Conference Special Issue
Authors

Author: Dolly Patricia Pardo MORA

Author: Oscar Julián MURILLO

Author: Mauricio Rey BUITRAGO

Author: Mónica LOSADA

Author: Jaime Fabian Cruz URIBE

Author: Karina Basso SANTIAGO

Author: Bruno José CONTI

Author: José Maurício SFORCIN

Bibtex @conference paper { jan489510, journal = {Journal of Apitherapy and Nature}, issn = {}, eissn = {2667-4734}, address = {Oktay YILDIZ}, year = {2018}, volume = {1}, pages = {57 - 57}, doi = {}, title = {Cytotoxic Activity of Four Propolis Colombian Samples Against Canine Osteosarcoma Cells}, key = {cite}, author = {MORA, Dolly Patricia Pardo and MURILLO, Oscar Julián and BUITRAGO, Mauricio Rey and LOSADA, Mónica and URIBE, Jaime Fabian Cruz and SANTIAGO, Karina Basso and CONTI, Bruno José and SFORCIN, José Maurício} }
APA MORA, D , MURILLO, O , BUITRAGO, M , LOSADA, M , URIBE, J , SANTIAGO, K , CONTI, B , SFORCIN, J . (2018). Cytotoxic Activity of Four Propolis Colombian Samples Against Canine Osteosarcoma Cells. Journal of Apitherapy and Nature, 1 (3), 57-57. Retrieved from http://dergipark.gov.tr/jan/issue/40577/489510
MLA MORA, D , MURILLO, O , BUITRAGO, M , LOSADA, M , URIBE, J , SANTIAGO, K , CONTI, B , SFORCIN, J . "Cytotoxic Activity of Four Propolis Colombian Samples Against Canine Osteosarcoma Cells". Journal of Apitherapy and Nature 1 (2018): 57-57 <http://dergipark.gov.tr/jan/issue/40577/489510>
Chicago MORA, D , MURILLO, O , BUITRAGO, M , LOSADA, M , URIBE, J , SANTIAGO, K , CONTI, B , SFORCIN, J . "Cytotoxic Activity of Four Propolis Colombian Samples Against Canine Osteosarcoma Cells". Journal of Apitherapy and Nature 1 (2018): 57-57
RIS TY - JOUR T1 - Cytotoxic Activity of Four Propolis Colombian Samples Against Canine Osteosarcoma Cells AU - Dolly Patricia Pardo MORA , Oscar Julián MURILLO , Mauricio Rey BUITRAGO , Mónica LOSADA , Jaime Fabian Cruz URIBE , Karina Basso SANTIAGO , Bruno José CONTI , José Maurício SFORCIN Y1 - 2018 PY - 2018 N1 - DO - T2 - Journal of Apitherapy and Nature JF - Journal JO - JOR SP - 57 EP - 57 VL - 1 IS - 3 SN - -2667-4734 M3 - UR - Y2 - 2019 ER -
EndNote %0 Journal of Apitherapy and Nature Cytotoxic Activity of Four Propolis Colombian Samples Against Canine Osteosarcoma Cells %A Dolly Patricia Pardo MORA , Oscar Julián MURILLO , Mauricio Rey BUITRAGO , Mónica LOSADA , Jaime Fabian Cruz URIBE , Karina Basso SANTIAGO , Bruno José CONTI , José Maurício SFORCIN %T Cytotoxic Activity of Four Propolis Colombian Samples Against Canine Osteosarcoma Cells %D 2018 %J Journal of Apitherapy and Nature %P -2667-4734 %V 1 %N 3 %R %U
ISNAD MORA, Dolly Patricia Pardo , MURILLO, Oscar Julián , BUITRAGO, Mauricio Rey , LOSADA, Mónica , URIBE, Jaime Fabian Cruz , SANTIAGO, Karina Basso , CONTI, Bruno José , SFORCIN, José Maurício . "Cytotoxic Activity of Four Propolis Colombian Samples Against Canine Osteosarcoma Cells". Journal of Apitherapy and Nature 1 / 3 (December 2018): 57-57.