Year 2018, Volume 1, Issue 3, Pages 59 - 59 2018-12-08

Mechanistic Studies of Cytotoxicity Induced by a Portuguese Propolis Extract, Using Saccharomyces cerevisiae as Eukaryotic Cell Model

Hugo ALVES [1] , Catarina PASSÃO [2] , Maria João SOUSA [3] , Cristina Almeida AGUIAR [4] , Ana CUNHA [5] , Rui Pedro OLIVEIRA [6]

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Propolis is a natural complex mixture produced by honey bees (particularly Apis mellifera L.) by collecting exudates from various plant sources. Characterized by a plethora of chemicals, propolis is generally rich in flavonoids, phenolic acids and terpene derivatives, bioactive compounds associated to it’s antimicrobial, anti-inflammatory, antimutagenic and antioxidant activities1. Previous work had shown that the ethanol extract of a sample from the Portuguese region of Beira Alta exhibited unique dual genotoxic and antigenotoxic effects using the yeast S. cerevisiae eukaryotic model2. In this work we prepared two ethanol extracts (EE) of propolis samples from Pereiro (P) - Beira Alta - collected in 2010 (P10.EE) and 2017 (P17.EE) to investigate the mechanisms of cytotoxicity and genotoxicity using specific S. cerevisiae mutants. While P17.EE didn’t show any toxic effect, yeast cells exposed to P10.EE showed a considerable decreased viability along time, assessed by colony-forming units. Interestingly, the oxidative stress response-defective mutant yap1 was more resistant than the wild type, suggesting that this cytotoxic effect was not mediated by oxidative stress. P.EE’s genotoxicity was also analysed by the nucleus-cytosolic translocation of NHP6A protein, considered a marker of necrosis. P10.EE induced NHP6A protein translocation to the cytoplasm, observed by fluorescence microscopy, suggesting that cytotoxicity of this extract was indeed mediated by necrosis. Although P17.EE didn’t seem to induce necrotic cell death, both extracts induced plasma membrane integrity loss, assessed by flow cytometry, using propidium iodide as marker. As recently observed for erythroleukemic cells with Brazilian propolis3, here we present the first evidence that also Portuguese propolis have necrotic-mediated cytotoxicity in yeast cells.

 

Acknowledgments: This work is supported by: European Investment Funds by FEDER/COMPETE/POCI– Operational Competitiveness and Internationalization Programme, under Project POCI-01-0145-FEDER-006958 and National Funds by FCT - Portuguese Foundation for Science and Technology, under the project UID/AGR/04033/2013.
Propolis, Saccharomyces cerevisiae
  • 1. Zabaiou N, Fouache A, Trousson A, Baron S, Zellagui A, Lahouel M, Lobaccaro JMA (2017) Chemistry and Physics of Lipids 207: 214-222.
  • 2. Cruz M, Antunes P, Paulo L, Ferreira AM, Cunha A, Almeida-Aguiar C, Oliveira R (2016) RSC Advances 55: 49806-49816.
  • 3 Bonamigo T, Campos JF, Alfredo TM, Balestieri JBP, Cardoso CAL, Paredes-Gamero EJ, Santos EL (2017) Oxidative Medicine and Cellular Longevity
Primary Language en
Subjects
Journal Section 2nd Propolis Conference Special Issue
Authors

Author: Hugo ALVES

Author: Catarina PASSÃO

Author: Maria João SOUSA

Author: Cristina Almeida AGUIAR

Author: Ana CUNHA

Author: Rui Pedro OLIVEIRA

Bibtex @conference paper { jan489517, journal = {Journal of Apitherapy and Nature}, issn = {}, eissn = {2667-4734}, address = {Oktay YILDIZ}, year = {2018}, volume = {1}, pages = {59 - 59}, doi = {}, title = {Mechanistic Studies of Cytotoxicity Induced by a Portuguese Propolis Extract, Using Saccharomyces cerevisiae as Eukaryotic Cell Model}, key = {cite}, author = {ALVES, Hugo and PASSÃO, Catarina and SOUSA, Maria João and AGUIAR, Cristina Almeida and CUNHA, Ana and OLIVEIRA, Rui Pedro} }
APA ALVES, H , PASSÃO, C , SOUSA, M , AGUIAR, C , CUNHA, A , OLIVEIRA, R . (2018). Mechanistic Studies of Cytotoxicity Induced by a Portuguese Propolis Extract, Using Saccharomyces cerevisiae as Eukaryotic Cell Model. Journal of Apitherapy and Nature, 1 (3), 59-59. Retrieved from http://dergipark.gov.tr/jan/issue/40577/489517
MLA ALVES, H , PASSÃO, C , SOUSA, M , AGUIAR, C , CUNHA, A , OLIVEIRA, R . "Mechanistic Studies of Cytotoxicity Induced by a Portuguese Propolis Extract, Using Saccharomyces cerevisiae as Eukaryotic Cell Model". Journal of Apitherapy and Nature 1 (2018): 59-59 <http://dergipark.gov.tr/jan/issue/40577/489517>
Chicago ALVES, H , PASSÃO, C , SOUSA, M , AGUIAR, C , CUNHA, A , OLIVEIRA, R . "Mechanistic Studies of Cytotoxicity Induced by a Portuguese Propolis Extract, Using Saccharomyces cerevisiae as Eukaryotic Cell Model". Journal of Apitherapy and Nature 1 (2018): 59-59
RIS TY - JOUR T1 - Mechanistic Studies of Cytotoxicity Induced by a Portuguese Propolis Extract, Using Saccharomyces cerevisiae as Eukaryotic Cell Model AU - Hugo ALVES , Catarina PASSÃO , Maria João SOUSA , Cristina Almeida AGUIAR , Ana CUNHA , Rui Pedro OLIVEIRA Y1 - 2018 PY - 2018 N1 - DO - T2 - Journal of Apitherapy and Nature JF - Journal JO - JOR SP - 59 EP - 59 VL - 1 IS - 3 SN - -2667-4734 M3 - UR - Y2 - 2019 ER -
EndNote %0 Journal of Apitherapy and Nature Mechanistic Studies of Cytotoxicity Induced by a Portuguese Propolis Extract, Using Saccharomyces cerevisiae as Eukaryotic Cell Model %A Hugo ALVES , Catarina PASSÃO , Maria João SOUSA , Cristina Almeida AGUIAR , Ana CUNHA , Rui Pedro OLIVEIRA %T Mechanistic Studies of Cytotoxicity Induced by a Portuguese Propolis Extract, Using Saccharomyces cerevisiae as Eukaryotic Cell Model %D 2018 %J Journal of Apitherapy and Nature %P -2667-4734 %V 1 %N 3 %R %U
ISNAD ALVES, Hugo , PASSÃO, Catarina , SOUSA, Maria João , AGUIAR, Cristina Almeida , CUNHA, Ana , OLIVEIRA, Rui Pedro . "Mechanistic Studies of Cytotoxicity Induced by a Portuguese Propolis Extract, Using Saccharomyces cerevisiae as Eukaryotic Cell Model". Journal of Apitherapy and Nature 1 / 3 (December 2018): 59-59.