Cilt 9, Sayı 2, Sayfalar 617 - 629

Effects of Different Doses of Curcumin on Apoptosis, Mitochondrial Oxidative Stress and Calcium Ion Influx in DBRG Glioblastoma Cells

Ahmi ÖZ [1] , Ömer ÇELİK [2] , İshak Suat ÖVEY [3]

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Transient Receptor Potential (TRP) channels superfamily has mostly calcium ion (Ca2+) permeable non-selective cation channels. Transient receptor potential melastatin subfamily 2 (TRPM2) is widely expressed in central nervous system. Intracellular Ca2+ concentration ([Ca2+]i) may change depend on TRPM2 and TRPM8 activations from extracellular liquid to cytosol. Curcumin as natural antioxidant shows phenolic structure, synthesized by Curcuma longa L. (Indian saffron, turmeric), has powerful non-enzymatically antioxidant effects. Low dose curcumin treatment can decrease calcium signaling via TRPM2 channel inhibition and prevent elevation of [Ca2+]i levels. Hence, we investigated effects of four different concentrations (5, 10, 25 and 50 µM) of curcumin on apoptosis and cell viability (MTT), reactive oxygen species (ROS) production, mitochondrial membrane potential levels, caspase 3 and caspase 9 values in DBRG glioblastoma cells.

            We found that curcumin reduces cell viability by concentration dependent manner. It was also observed that curcumin induces apoptosis via caspase 3 and 9 related pathways. However, it was not found any direct relationship between the effect of increased concentrations of curcumin and inhibition or activation of TRPM2 mediated Ca2+ signaling in the DBTRG cells. The [Ca2+]i concentration was lower in 5 µM group as compare to control group. Curcumin acted important role on decrease of mitochondrial membrane potential and intracellular ROS production in the cells. Moreover, curcumin treatment markedly supported GSH concentration levels in the cells. 

In conclusion, it was firstly assessed the effects of different doses of curcumin on TRPM2 mediated calcium signaling and interaction with various apoptosis parameters in DBTRG glioblastoma cells.

Curcumin,cell viability,Ca2+ signaling,TRPM2,DBTRG cells
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Konular Yaşam Bilimleri
Dergi Bölümü Original Articles
Yazarlar

Orcid: orcid.org/0000-0003-1881-8460
Yazar: Ahmi ÖZ
E-posta: ahmioz@sdu.edu.tr
Kurum: Süleyman Demirel Üniversitesi
Ülke: Turkey


Orcid: orcid.org/0000-0002-9262-996X
Yazar: Ömer ÇELİK
E-posta: omercelik@sdu.edu.tr
Kurum: Süleyman Demirel Üniversitesi
Ülke: Turkey


Yazar: İshak Suat ÖVEY
E-posta: ishaksuatovey@gmail.com
Kurum: Süleyman Demirel Üniversitesi
Ülke: Turkey


Bibtex @araştırma makalesi { jcnos330858, journal = {Journal of Cellular Neuroscience and Oxidative Stress}, issn = {}, address = {Hücresel Sinir Bilimleri ve Oksidatif Stres Derneği}, year = {}, volume = {9}, pages = {617 - 629}, doi = {}, title = {Effects of Different Doses of Curcumin on Apoptosis, Mitochondrial Oxidative Stress and Calcium Ion Influx in DBRG Glioblastoma Cells}, language = {en}, key = {cite}, author = {ÖVEY, İshak Suat and ÇELİK, Ömer and ÖZ, Ahmi} }
APA ÖZ, A , ÇELİK, Ö , ÖVEY, İ . (). Effects of Different Doses of Curcumin on Apoptosis, Mitochondrial Oxidative Stress and Calcium Ion Influx in DBRG Glioblastoma Cells. Journal of Cellular Neuroscience and Oxidative Stress, 9 (2), 617-629. Retrieved from http://dergipark.gov.tr/jcnos/issue/31056/330858
MLA ÖZ, A , ÇELİK, Ö , ÖVEY, İ . "Effects of Different Doses of Curcumin on Apoptosis, Mitochondrial Oxidative Stress and Calcium Ion Influx in DBRG Glioblastoma Cells". Journal of Cellular Neuroscience and Oxidative Stress 9 (): 617-629 <http://dergipark.gov.tr/jcnos/issue/31056/330858>
Chicago ÖZ, A , ÇELİK, Ö , ÖVEY, İ . "Effects of Different Doses of Curcumin on Apoptosis, Mitochondrial Oxidative Stress and Calcium Ion Influx in DBRG Glioblastoma Cells". Journal of Cellular Neuroscience and Oxidative Stress 9 (): 617-629
RIS TY - JOUR T1 - Effects of Different Doses of Curcumin on Apoptosis, Mitochondrial Oxidative Stress and Calcium Ion Influx in DBRG Glioblastoma Cells AU - Ahmi ÖZ , Ömer ÇELİK , İshak Suat ÖVEY Y1 - 2017 PY - 2017 N1 - DO - T2 - Journal of Cellular Neuroscience and Oxidative Stress JF - Journal JO - JOR SP - 617 EP - 629 VL - 9 IS - 2 SN - -2149-7222 M3 - UR - Y2 - 2017 ER -
EndNote %0 Journal of Cellular Neuroscience and Oxidative Stress Effects of Different Doses of Curcumin on Apoptosis, Mitochondrial Oxidative Stress and Calcium Ion Influx in DBRG Glioblastoma Cells %A Ahmi ÖZ , Ömer ÇELİK , İshak Suat ÖVEY %T Effects of Different Doses of Curcumin on Apoptosis, Mitochondrial Oxidative Stress and Calcium Ion Influx in DBRG Glioblastoma Cells %D 2017 %J Journal of Cellular Neuroscience and Oxidative Stress %P -2149-7222 %V 9 %N 2 %R %U