Yıl 2017, Cilt 4, Sayı 1, Sayfalar 133 - 148 2016-12-08

The Adsorption of Calmoduline via Nicotinamide Immobilized Poly(HEMA-GMA) Cryogels

Kadir Erol [1]

441 337

The separation and purification methods for the isolation of an important biomolecule calmoduline protein is extremely important. Among these methods, the adsorption technique is extremely popular, and the cryogels as adsorbents with the macro porous structure and interconnected flow channels cryogel they have are preferred in this field. In this study, the adsorption of calmoduline via Ca(II) immobilized poly (2-hydroxyethyl methacrylate-glycidyl methacrylate), poly (HEMA-GMA), cryogels through changing interaction time, calmoduline initial concentration and temperature conditions. For the characterization of cryogels, the swelling test, Fourier Transform Infrared (FT-IR) Spectroscopy, Scanning Electron Microscopy (SEM), surface area (BET), elemental analysis and ICP-OES methods were performed. Nicotinamide molecule was used as Ca (II) chelating agent and the adsorption capacity of the cryogels was estimated as 1.812 mg calmoduline / g cryogel. The adsorption models of the adsorption reaction were examined by the Langmuir and Freundlich isotherm models and was determined to be more appropriate for Langmuir isotherm model.

Adsorption, calmoduline; cryogel; nicotinamide.
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Yazar: Kadir Erol

Bibtex @ { jotcsa287321, journal = {Journal of the Turkish Chemical Society, Section A: Chemistry}, issn = {}, eissn = {2149-0120}, address = {Türkiye Kimya Derneği}, year = {2016}, volume = {4}, pages = {133 - 148}, doi = {10.18596/jotcsa.287321}, title = {The Adsorption of Calmoduline via Nicotinamide Immobilized Poly(HEMA-GMA) Cryogels}, key = {cite}, author = {Erol, Kadir} }
APA Erol, K . (2016). The Adsorption of Calmoduline via Nicotinamide Immobilized Poly(HEMA-GMA) Cryogels. Journal of the Turkish Chemical Society, Section A: Chemistry, 4 (1), 133-148. DOI: 10.18596/jotcsa.287321
MLA Erol, K . "The Adsorption of Calmoduline via Nicotinamide Immobilized Poly(HEMA-GMA) Cryogels". Journal of the Turkish Chemical Society, Section A: Chemistry 4 (2016): 133-148 <http://dergipark.gov.tr/jotcsa/issue/27291/287321?publisher=turchemsoc>
Chicago Erol, K . "The Adsorption of Calmoduline via Nicotinamide Immobilized Poly(HEMA-GMA) Cryogels". Journal of the Turkish Chemical Society, Section A: Chemistry 4 (2016): 133-148
RIS TY - JOUR T1 - The Adsorption of Calmoduline via Nicotinamide Immobilized Poly(HEMA-GMA) Cryogels AU - Kadir Erol Y1 - 2016 PY - 2016 N1 - doi: 10.18596/jotcsa.287321 DO - 10.18596/jotcsa.287321 T2 - Journal of the Turkish Chemical Society, Section A: Chemistry JF - Journal JO - JOR SP - 133 EP - 148 VL - 4 IS - 1 SN - -2149-0120 M3 - doi: 10.18596/jotcsa.287321 UR - http://dx.doi.org/10.18596/jotcsa.287321 Y2 - 2018 ER -
EndNote %0 Journal of the Turkish Chemical Society, Section A: Chemistry The Adsorption of Calmoduline via Nicotinamide Immobilized Poly(HEMA-GMA) Cryogels %A Kadir Erol %T The Adsorption of Calmoduline via Nicotinamide Immobilized Poly(HEMA-GMA) Cryogels %D 2016 %J Journal of the Turkish Chemical Society, Section A: Chemistry %P -2149-0120 %V 4 %N 1 %R doi: 10.18596/jotcsa.287321 %U 10.18596/jotcsa.287321
ISNAD Erol, Kadir . "The Adsorption of Calmoduline via Nicotinamide Immobilized Poly(HEMA-GMA) Cryogels". Journal of the Turkish Chemical Society, Section A: Chemistry 4 / 1 (Aralık 2016): 133-148. http://dx.doi.org/10.18596/jotcsa.287321