Yıl 2018, Cilt 19, Sayı 3, Sayfalar 98 - 108 2018-07-23

KADMİYUMA MARUZ BIRAKILAN DİYABETİK RATLARA ALFA LİPOİK ASİT VE İNSÜLİN UYGULANMASININ OLASI DÜZENLEYİCİ ETKİLERİ
POSSIBLE REGULATORY EFFECTS OF APPLICATION OF ALPHA LIPOIC ACID AND INSULIN AGAINST CADMIUM EXPOSED DIABETIC RATS

Neşe Kılıç [1] , Fahrettin Akyüz [2] , Neslihan Tekin [3]

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AMAÇ: Kadmiyuma (Cd) çevresel maruziyet hiperglisemi ve azalmış serum insülini ile ilişkilidir. Bu çalışma, Cd’ye maruz kalmış diyabetik ratlarda Lipoik Asit (LA) ve insülinin glikolitik enzimler, karaciğer marker enzimleri ve lipidler üzerindeki etkilerini değerlendirmek için tasarlanmıştır.

GEREÇ VE YÖNTEM: Erkek Wistar sıçanları 7 gruba ayrıldı (n = 8). Gruplar kontrol, diyabetik kontrol, diyabet + CdCl2, diyabet + insülin, diyabet + CdCl2 + insülin, diyabet + CdCl2 + LA ve diyabet + CdCl2 + insülin + LA gruplarından oluştu. Tip 1 diyabet, 6 gruba intraperitoneal (i.p.) streptozotosin (STZ) (65 mg / kg) enjeksiyonu ile indüklendi. İnsülin (4 IU/kg/gün), insülin ile tedavi edilen gruplara subkutan (s.c.) verildi. CdCl2 (1,2 mg/kg/gün), CdCl2 ile tedavi edilen gruplara s.c. verildi. LA (100 mg/ kg/gün), LA ile tedavi edilen gruplara i.p. verildi. CdCl2 ve insülin tedavisi, intraperitoneal STZ enjeksiyonundan 2 gün sonra başlatıldı ve 3 hafta sürdürüldü. Serum glukoz, AST, ALT, ALP, BUN, LDL, HDL ve TG düzeyleri, tam kan HbA1c düzeyi ve karaciğer heksokinaz (HK), piruvat kinaz (PK) ve Na+/K+ ATPaz aktivitesi değerlendirildi.

BULGULAR: Diyabetik grupta serum glukoz, HbA1c, TG, LDL, AST, ALT, ALP ve BUN düzeyleri kontrol grubuna göre daha yüksek bulundu, ancak HDL daha düşüktü. Diyabetik kontrol grubu karaciğer dokusunda Na+/K+ ATPaz, HK ve PK aktivitesi azaldı. Diabetik + CdCl2 ve Diabetik+İnsulin+CdCl2 gruplarında karaciğerde PK, HK ve Na+/K+ ATPaz aktivitesi arttı. İnsülin ve LA ile tedavi edilen gruplarda HK, PK ve Na+/K+ ATPaz aktivitelerinde diyabetik kontrol grubu ile karşılaştırıldığında artış saptandı.

SONUÇ: Bu sonuçlar insülin ve LA uygulamasının Cd ve STZ’nin neden olduğu karaciğer hasarına karşı etkili bir terapötik müdahale olabileceğini düşündürmektedir.

OBJECTIVE: Environmental exposure to the cadmium (Cd), is associated with hyperglycemia and reduced serum insulin. This investigation was planned to assess the effects of Lipoic Acid (LA) and insulin on glycolytic enzymes, liver marker enzymes and lipids in Cd exposed diabetic rats.

MATERIAL AND METHODS: Male Wistar rats were separated into 7 groups (n=8 in each group). Groups were designed as control, diabetic control, diabetic + CdCl2, diabetic + insulin, diabetic + CdCl2 + insulin, diabetic + CdCl2 + LA, anddiabetic + CdCl2 + insulin + LA groups. Type 1 diabetes was established by intraperitoneal (i.p.) injection of streptozotocin (STZ) (65 mg/kg) into 6 groups. Insulin (4 IU/kg/day) was given subcutaneously (s.c.) to insulin treated groups. CdCl2 (1,2 mg/kg/day) was given s.c. to CdCl2 treated groups. LA (100 mg/kg/day) was given i.p. to LA treated groups. CdCl2, LA, and insulin treatment were started 2 days after intraperitoneal STZ injection and continued for 3 weeks. Serum glucose, AST, ALT, BUN, LDL, HDL, and TG levels and liver hexokinase (HK), pyruvate kinase (PK), whole blood HbA1c level, and Na+/K+ATPase activity were evaluated.

RESULTS: In diabetic group, serum glucose, HbA1c, TG, LDL, AST, ALT, ALP, and BUN levels were higher than cont- rol, but HDL was lower. In liver tissue, activities of Na+/ K+ATPase, HK and PK activities were decreased in dia- betic control group. PK, HK and Na+/K+ATPase activities were increased in liver in diabetic+CdCl2 and Diabeti- c+Insulin+CdCl2 groups. An increase was determined in activities of HK, PK, and Na+/K+ATPase in insulin and LA treated groups compared with diabetic control group.

CONCLUSIONS: These results suggest that application of insulin and LA could be an effective therapeutic intervention against liver injury caused by Cd and STZ.

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Dergi Bölümü Makaleler-Araştırma Yazıları
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Yazar: Neşe Kılıç
Kurum: ESKİŞEHİR OSMANGAZİ ÜNİVERSİTESİ, TIP FAKÜLTESİ
Ülke: Turkey


Yazar: Fahrettin Akyüz
Kurum: ESKİŞEHİR OSMANGAZİ ÜNİVERSİTESİ, TIP FAKÜLTESİ
Ülke: Turkey


Yazar: Neslihan Tekin (Sorumlu Yazar)
Kurum: AKSARAY ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ

Bibtex @araştırma makalesi { kocatepetip425001, journal = {Kocatepe Tıp Dergisi}, issn = {1302-4612}, eissn = {2149-7869}, address = {Afyon Kocatepe Üniversitesi}, year = {2018}, volume = {19}, pages = {98 - 108}, doi = {10.18229/kocatepetip.425001}, title = {POSSIBLE REGULATORY EFFECTS OF APPLICATION OF ALPHA LIPOIC ACID AND INSULIN AGAINST CADMIUM EXPOSED DIABETIC RATS}, key = {cite}, author = {Kılıç, Neşe and Tekin, Neslihan and Akyüz, Fahrettin} }
APA Kılıç, N , Akyüz, F , Tekin, N . (2018). POSSIBLE REGULATORY EFFECTS OF APPLICATION OF ALPHA LIPOIC ACID AND INSULIN AGAINST CADMIUM EXPOSED DIABETIC RATS. Kocatepe Tıp Dergisi, 19 (3), 98-108. DOI: 10.18229/kocatepetip.425001
MLA Kılıç, N , Akyüz, F , Tekin, N . "POSSIBLE REGULATORY EFFECTS OF APPLICATION OF ALPHA LIPOIC ACID AND INSULIN AGAINST CADMIUM EXPOSED DIABETIC RATS". Kocatepe Tıp Dergisi 19 (2018): 98-108 <http://dergipark.gov.tr/kocatepetip/issue/38516/425001>
Chicago Kılıç, N , Akyüz, F , Tekin, N . "POSSIBLE REGULATORY EFFECTS OF APPLICATION OF ALPHA LIPOIC ACID AND INSULIN AGAINST CADMIUM EXPOSED DIABETIC RATS". Kocatepe Tıp Dergisi 19 (2018): 98-108
RIS TY - JOUR T1 - POSSIBLE REGULATORY EFFECTS OF APPLICATION OF ALPHA LIPOIC ACID AND INSULIN AGAINST CADMIUM EXPOSED DIABETIC RATS AU - Neşe Kılıç , Fahrettin Akyüz , Neslihan Tekin Y1 - 2018 PY - 2018 N1 - doi: 10.18229/kocatepetip.425001 DO - 10.18229/kocatepetip.425001 T2 - Kocatepe Tıp Dergisi JF - Journal JO - JOR SP - 98 EP - 108 VL - 19 IS - 3 SN - 1302-4612-2149-7869 M3 - doi: 10.18229/kocatepetip.425001 UR - http://dx.doi.org/10.18229/kocatepetip.425001 Y2 - 2018 ER -
EndNote %0 Kocatepe Tıp Dergisi POSSIBLE REGULATORY EFFECTS OF APPLICATION OF ALPHA LIPOIC ACID AND INSULIN AGAINST CADMIUM EXPOSED DIABETIC RATS %A Neşe Kılıç , Fahrettin Akyüz , Neslihan Tekin %T POSSIBLE REGULATORY EFFECTS OF APPLICATION OF ALPHA LIPOIC ACID AND INSULIN AGAINST CADMIUM EXPOSED DIABETIC RATS %D 2018 %J Kocatepe Tıp Dergisi %P 1302-4612-2149-7869 %V 19 %N 3 %R doi: 10.18229/kocatepetip.425001 %U 10.18229/kocatepetip.425001
ISNAD Kılıç, Neşe , Akyüz, Fahrettin , Tekin, Neslihan . "KADMİYUMA MARUZ BIRAKILAN DİYABETİK RATLARA ALFA LİPOİK ASİT VE İNSÜLİN UYGULANMASININ OLASI DÜZENLEYİCİ ETKİLERİ". Kocatepe Tıp Dergisi 19 / 3 (Temmuz 2018): 98-108. http://dx.doi.org/10.18229/kocatepetip.425001