Yıl 2018, Cilt 11, Sayı 1, Sayfalar 11 - 17 2018-03-01

Geleneksel Türk Süt Ürünü Kaymaktan Escherichia coli O157'nin İlk Bildirimi
First Report of Escherichia coli O157 From Traditional Turkish Dairy Product Kaymak

Didem SAĞLAM [1] , Esra ŞEKER [2]

90 217

Bu çalışmada, Afyonkarahisar'da satılan geleneksel bir Türk süt ürünü olan kaymaktan E. coli O157:H7/H- serotipinin izolasyonu, izole edilen suşlarda Stx1, Stx2, ehlyA ve eaeA genlerinin PZR ile araştırılması ve suşların Türkiye'de yaygın olarak kullanılan bazı antibiyotiklere karşı antibiyotik dirençliliğinin belirlenmesi amaçlandı. Bu amaçla, Afyonkarahisar'ın merkez ve köylerindeki halk pazarlarında satılan toplam 100 kaymak örneği toplandı. Konvansiyonel kültür yöntemleri ve serolojik doğrulama testleri kullanılarak 100 kaymak örneğinin 3'ünden E. coli O157 izole edildi. İzole edilen 3 suşta Stx1, Stx2, ehlyA ve eaeA genlerinin varlığı PZR ile araştırıldı. Üç suşun tamamı Stx1 ve ehlyA genlerine sahipken, suşlarda Stx2 ve eaeA genleri bulunmadı. Suşların antibiyotik dirençliliği Kirby-Bauer disk difüzyon testi kullanılarak araştırıldı. Suşların tamamı sefazolin, sefoksitin ve seftiofura dirençli iken, suşlarda ayrıca ampisilin (%66,7), sefalothin (%66,7), seftriakson (%33,3), nalidiksik asit (%33,3) ve trimetoprim/sulfametoksazole (%33,3) karşı da direnç tespit edildi. Sunulan çalışma, Türkiye'de kaymaklarda E. coli O157'nin ve serotipin virulens genlerinin varlığını gösteren ilk araştırmadır.
The aim of this study was to isolate the E. coli O157:H7/H- serotype from traditional Turkish dairy product kaymak sold in Afyonkarahisar, investigate the Stx1, Stx2, ehlyA and eaeA genes in isolated strains by PCR and determine the antibiotic resistance of strains to some antibiotics commonly used in Turkey. For this purpose, a total of 100 kaymak samples sold in public bazaars located center town and villages of Afyonkarahisar were collected. E. coli O157 was isolated from 3 of 100 samples by using conventional culture methods and serological confirmation tests. The presence of Stx1, Stx2, ehlyA and eaeA genes in the isolated 3 strains was investigated by PCR. While all of 3 strains harboured the Stx1 and ehlyA genes, the Stx2 and eaeA genes were not found in the strains. The antibiotic resistance of strains was investigated by Kirby-Bauer disc diffusion test. While all of strains were resistant to cephazoline, cefoxitin and ceftiofur, the resistance was also determined in the strains to ampicillin (66.7%), cephalothin (66.7%), ceftriaxone (33.3%), nalidixic acid (33.3%) and trimethoprim/sulphamethoxazole (33.3%). The present study is the first investigation showing the presence of E. coli O157 and its virulence genes in the kaymak in Turkey.
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Birincil Dil tr
Konular Yaşam Bilimleri
Dergi Bölümü ARAŞTIRMA MAKALESİ
Yazarlar

Yazar: Didem SAĞLAM

Yazar: Esra ŞEKER

Bibtex @araştırma makalesi { kvj370138, journal = {Kocatepe Veteriner Dergisi}, issn = {1308-1594}, eissn = {2147-6853}, address = {Afyon Kocatepe Üniversitesi}, year = {2018}, volume = {11}, pages = {11 - 17}, doi = {}, title = {Geleneksel Türk Süt Ürünü Kaymaktan Escherichia coli O157'nin İlk Bildirimi}, key = {cite}, author = {ŞEKER, Esra and SAĞLAM, Didem} }
APA SAĞLAM, D , ŞEKER, E . (2018). Geleneksel Türk Süt Ürünü Kaymaktan Escherichia coli O157'nin İlk Bildirimi. Kocatepe Veteriner Dergisi, 11 (1), 11-17. Retrieved from http://dergipark.gov.tr/kvj/issue/33193/370138
MLA SAĞLAM, D , ŞEKER, E . "Geleneksel Türk Süt Ürünü Kaymaktan Escherichia coli O157'nin İlk Bildirimi". Kocatepe Veteriner Dergisi 11 (2018): 11-17 <http://dergipark.gov.tr/kvj/issue/33193/370138>
Chicago SAĞLAM, D , ŞEKER, E . "Geleneksel Türk Süt Ürünü Kaymaktan Escherichia coli O157'nin İlk Bildirimi". Kocatepe Veteriner Dergisi 11 (2018): 11-17
RIS TY - JOUR T1 - Geleneksel Türk Süt Ürünü Kaymaktan Escherichia coli O157'nin İlk Bildirimi AU - Didem SAĞLAM , Esra ŞEKER Y1 - 2018 PY - 2018 N1 - DO - T2 - Kocatepe Veteriner Dergisi JF - Journal JO - JOR SP - 11 EP - 17 VL - 11 IS - 1 SN - 1308-1594-2147-6853 M3 - UR - Y2 - 2017 ER -
EndNote %0 Kocatepe Veteriner Dergisi Geleneksel Türk Süt Ürünü Kaymaktan Escherichia coli O157'nin İlk Bildirimi %A Didem SAĞLAM , Esra ŞEKER %T Geleneksel Türk Süt Ürünü Kaymaktan Escherichia coli O157'nin İlk Bildirimi %D 2018 %J Kocatepe Veteriner Dergisi %P 1308-1594-2147-6853 %V 11 %N 1 %R %U
ISNAD SAĞLAM, Didem , ŞEKER, Esra . "Geleneksel Türk Süt Ürünü Kaymaktan Escherichia coli O157'nin İlk Bildirimi". Kocatepe Veteriner Dergisi 11 / 1 (Mart 2018): 11-17.