Yıl 2018, Cilt 11, Sayı 1, Sayfalar 86 - 95 2018-03-01

İnfeksiyonların Tanısında En Çok Kullanılan İzotermal Amplifikasyon Yöntemleri
Isothermal Amplification Methods Most Used in the Diagnosis of Infections

Mehmet ÜVEY [1] , Nilgün ÜNAL [2]

150 292

Nükleik asit amplifikasyonu, enfeksiyonların, tümörlerin, genetik anormalliklerin tanısında ve adli tıpta en fazla kullanılan yöntemlerdendir. İzotermal amplifikasyon yöntemleri, Polimeraz Zincir Reaksiyonu (PZR) metoduna göre sabit sıcaklıkta yapılmasıyla karmaşıklığı azaltır. Bu yöntemler, kullanılan enzim çeşitliliği, primerler, duyarlılıkları ile özgüllükleri bakımından farklılıklar göstermektedir. Bu derlemede izotermal amplifikasyon yöntemlerinden en sık kullanılanları, prensipleri, kullanım alanları ve getirdikleri yenilikler ele alınmıştır.
Isothermal amplification of nucleic acid is one of the most commonly used methods to identify the infections, tumors, genetic abnormalities, and in forensic medicine. As isothermal amplification methods can be performed at one reaction temperature under simple conditions they are less complex in comparison to Polymerase Chain Reaction (PCR). These methods differ from each other with the diversity of enzymes, primers used, sensitivity and specificity. In this review, the most commonly used isothermal amplification methods, their principles, areas of use and innovations brought about are discussed.
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Dergi Bölümü DERLEME
Yazarlar

Yazar: Mehmet ÜVEY

Yazar: Nilgün ÜNAL (Sorumlu Yazar)

Bibtex @derleme { kvj374662, journal = {Kocatepe Veteriner Dergisi}, issn = {1308-1594}, eissn = {2147-6853}, address = {Afyon Kocatepe Üniversitesi}, year = {2018}, volume = {11}, pages = {86 - 95}, doi = {}, title = {İnfeksiyonların Tanısında En Çok Kullanılan İzotermal Amplifikasyon Yöntemleri}, key = {cite}, author = {ÜNAL, Nilgün and ÜVEY, Mehmet} }
APA ÜVEY, M , ÜNAL, N . (2018). İnfeksiyonların Tanısında En Çok Kullanılan İzotermal Amplifikasyon Yöntemleri. Kocatepe Veteriner Dergisi, 11 (1), 86-95. Retrieved from http://dergipark.gov.tr/kvj/issue/33193/374662
MLA ÜVEY, M , ÜNAL, N . "İnfeksiyonların Tanısında En Çok Kullanılan İzotermal Amplifikasyon Yöntemleri". Kocatepe Veteriner Dergisi 11 (2018): 86-95 <http://dergipark.gov.tr/kvj/issue/33193/374662>
Chicago ÜVEY, M , ÜNAL, N . "İnfeksiyonların Tanısında En Çok Kullanılan İzotermal Amplifikasyon Yöntemleri". Kocatepe Veteriner Dergisi 11 (2018): 86-95
RIS TY - JOUR T1 - İnfeksiyonların Tanısında En Çok Kullanılan İzotermal Amplifikasyon Yöntemleri AU - Mehmet ÜVEY , Nilgün ÜNAL Y1 - 2018 PY - 2018 N1 - DO - T2 - Kocatepe Veteriner Dergisi JF - Journal JO - JOR SP - 86 EP - 95 VL - 11 IS - 1 SN - 1308-1594-2147-6853 M3 - UR - Y2 - 2017 ER -
EndNote %0 Kocatepe Veteriner Dergisi İnfeksiyonların Tanısında En Çok Kullanılan İzotermal Amplifikasyon Yöntemleri %A Mehmet ÜVEY , Nilgün ÜNAL %T İnfeksiyonların Tanısında En Çok Kullanılan İzotermal Amplifikasyon Yöntemleri %D 2018 %J Kocatepe Veteriner Dergisi %P 1308-1594-2147-6853 %V 11 %N 1 %R %U
ISNAD ÜVEY, Mehmet , ÜNAL, Nilgün . "İnfeksiyonların Tanısında En Çok Kullanılan İzotermal Amplifikasyon Yöntemleri". Kocatepe Veteriner Dergisi 11 / 1 (Mart 2018): 86-95.