Yıl 2018, Cilt 11, Sayı 3, Sayfalar 215 - 222 2018-09-01

Effects of Curcumin on A Renal Ischemia/Reperfusion Injury in Rats
Ratlarda Renal İskemi/Reperfüzyon Hasarında Curcumin'in Etkileri

Zülfükar SARITAŞ [1] , Hazen SARITAŞ [2] , Musa KORKMAZ [3] , M.Fatih BOZKURT [4] , Aziz BÜLBÜL [5] , Rengin ELSÜRER AFŞAR [6] , Tuba Berra SARITAŞ [7]

70 115

This study aimed to investigate the biochemical and histopathological effects of curcumin on Renal Ischemia/Reperfusion Injury. Twenty-four female Wistar rats were used in the study and divided into control (C), sham, ischemia/reperfusion (I/R), and curcimin (CUR) groups (n=6). Laparotomy was performed under general anesthesia in I/R and CUR groups than the left renal pedicle was dissected it was clamped. Then, 1-h ischemia and 6-h reperfusion were applied. 500 mg/kg Curcumin was given intraperitoneally to the CUR group after ischemia application. MPO, IMA, MDA, NO, SOD, GPx, AOS, urea, and creatinine levels were measured in serum samples. MPO, MDA, NO, SOD, GPx, and AOS were also measured in tissue samples. The histopathological examination was performed. Serum and tissue AOS levels were significantly higher in the CUR group than in the I/R group (P<0.05). Tissue NO levels were significantly lower in the CUR group than in the I/R group (15.30±5.41 and 4.8±1.37, respectively) (P<0.05). Histopathological scores were also significantly lower in the CUR group than in the I/R group (P<0.05). The results showed that curcumin prevented I/R damage by decreasing oxidative stress in serum and tissue samples in rat renal I/R model. 

Bu araştırmada, Renal İskemi/Reperfüzyon Hasarı Modelinde Curcumin'in etkilerini biyokimyasal ve histopatolojik olarak araştırmak amaçlanmıştır. Çalışmada ratlar kontrol (C), sham, iskemi repürfüzyon (I/R) ve curcumin (CUR)  grubu olmak üzere (n=6) toplam 24 dişi Wistar Rat kullanılmıştır. Genel anestezi altında Grup I/R ve CUR da laparatomi uygulanarak sol böbrek pedikülü diseke edilmiş ve böbrek arteri klempe edilerek 1 saat iskemi ve 6 saat reperfüzyon gerçekleştirilmiştir. Grup CUR da 500 mg/kg Curcumin iskemi sonrası intraperitonal olarak verilmiştir. Serum MPO, IMA, MDA, NO, SOD, GPx, AOS , üre ve kreatinin ölçümleri gerçekleştirilmiştir. Doku örneklerinde MPO, MDA, NO, SOD, GPx, AOS ölçülmüştür. Histopatolojik inceleme ile böbrek dokusunda I/R hasarı skorlanmıştır. Serum ve doku AOS Grup I/R Grup CUR ile karşılaştırıldığında istatistiksel önemi olacak şekilde Grup CUR'de yüksel bulunmuştur (p<0.05). Doku NO düzeyi I/R grubu ile karşılaştırıldığında Grup CUR’da istatistiksel önemi olacak şekilde düşük bulunmuştur (15,30±5,41, 4,8±1,37) (p<0.05). Histopatolojik skorlamada Grup CUR, Grup I/R ile karşılaştırıldığında istatistiksel olarak anlamlı olacak şekilde düşük bulunmuştur (p<0.05). Sonuç olarak; bu araştırmada Curcumin’in Renal I/R hasarı modelinde serum ve doku örneklerinde oksidatif stresi azaltarak I/R hasarını önlemede olumlu etkisi olduğu ve histopatolojik inceleme ile de Curcumin'in bu olumlu etkisi desteklenmiştir.


  • Adewusi E, Afolayan A. Antibacterial, antifungal and antioxidant activity of the roots and leaves of Pelargonium reniforme Curtis (Geraniaceae). African Journal of Biotechnology. 2009; 8.
  • Anwaruddin S, Januzzi Jr JL, Baggish AL, Lewandrowski EL, Lewandrowski KB. Ischemia-modified albumin improves the usefulness of standard cardiac biomarkers for the diagnosis of myocardial ischemia in the emergency department setting. American journal of clinical pathology. 2005; 123, 140-145.
  • Atthe BK, Babsky AM, Hopewell PN, Phillips CL, Molitoris BA, Bansal N. Early monitoring of acute tubular necrosis in the rat kidney by 23Na-MRI. American Journal of Physiology-Renal Physiology. 2009; 297, F1288-F1298.
  • Dagher PC. Apoptosis in ischemic renal injury: roles of GTP depletion and p53. Kidney international. 2004; 66, 506-509.
  • Döslüoglu HH, Aktan AÖ, Yeğen C, Okboy N, Yalçm A, Yahn R, Ercan S. The cytoprotective effects of verapamil and iloprost (ZK 36374) on ischemia/reperfusion injury of kidneys. Transplant international. 1993; 6, 138-142.
  • Draper H, Hadley M. Malondialdehyde determination as index of lipid Peroxidation. Methods in enzymology. 1990; 186, 421-431.
  • Ellidag HY, Eren E, Aydin O, Akgol E, Yalcinkaya S, Sezer C, Yilmaz N. Ischemia modified albumin levels and oxidative stress in patients with bladder cancer. Asian Pac J Cancer Prev. 2013; 14, 2759-2763.
  • Erkasap S, Erkasap N, Koken T, Kahraman A, Uzuner K, Yazihan N, Ates E. Effect of leptin on renal ischemia-reperfusion damage in rats. Journal of physiology and biochemistry. 2004; 60, 79-84.
  • Grossini E, Molinari C, Pollesello P, Bellomo G, Valente G, Mary D, Vacca G, Caimmi P. Levosimendan protection against kidney ischemia/reperfusion injuries in anesthetized pigs. Journal of Pharmacology and Experimental Therapeutics. 2012; 342, 376-388.
  • Guan X, Dei‐Anane G, Bruns H, Chen J, Nickkholgh A, Liang R, Gross ML, Kern M, Ludwig J, Büchler MW. Danshen protects kidney grafts from ischemia/reperfusion injury after experimental transplantation. Transplant International. 2009; 22, 232-241.
  • He X, Xu X, Fan M, Chen X, Sun X, Luo G, Chen L, Mu Q, Feng Y, Mao Q. Preconditioning with hyperbaric oxygen induces tolerance against renal ischemia-reperfusion injury via increased expression of heme oxygenase-1. Journal of Surgical Research. 2011; 170, e271-e277.
  • İhtiyar E, Yaşar NF, Erkasap N, Köken T, Tosun M, Öner S, Erkasap S. Effects of doxycycline on renal ischemia reperfusion injury induced by abdominal compartment syndrome. Journal of Surgical Research. 2011; 167, 113-120.
  • Kettle A, Winterbourn C. Myeloperoxidase: a key regulator of neutrophil oxidant production. Redox Report. 1997; 3, 3-15.
  • Korkmaz A, Kolankaya D. The protective effects of ascorbic acid against renal ischemia-reperfusion injury in male rats. Renal failure. 2009; 31, 36-43.
  • Kucuk A, Kabadere S, Tosun M, Koken T, Kinaci M, Isikli B, Erkasap N. Protective effects of doxycycline in ischemia/reperfusion injury on kidney. Journal of physiology and biochemistry. 2009; 65, 183-191.
  • Kumar S, Allen DA, Kieswich JE, Patel NS, Harwood S, Mazzon E, Cuzzocrea S, Raftery MJ, Thiemermann C, Yaqoob MM. Dexamethasone ameliorates renal ischemia-reperfusion injury. Journal of the American Society of Nephrology. 2009; 20, 2412-2425.
  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. Journal of biological chemistry. 1951; 193, 265-275.
  • Miquel J, Bernd A, Sempere J, Dıaz-Alperi J, Ramırez A. The curcuma antioxidants: pharmacological effects and prospects for future clinical use. A review. Archives of gerontology and geriatrics. 2002; 34, 37-46.
  • Miranda KM, Espey MG, Wink DA. A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric oxide. 2001; 5, 62-71.
  • Pandya U, Saini MK, Jin GF, Awasthi S, Godley BF, Awasthi YC. Dietary curcumin prevents ocular toxicity of naphthalene in rats. Toxicology letters. 2000; 115, 195-204.
  • Pompermayer K, Souza DG, Lara GG, Silveira KD, Cassali GD, Andrade AA, Bonjardim CA, Passaglio KT, Assreuy J, Cunha FQ. The ATP-sensitive potassium channel blocker glibenclamide prevents renal ischemia/reperfusion injury in rats. Kidney international. 2005; 67, 1785-1796.
  • Roy D, Quiles J, Gaze D, Collinson P, Kaski J, Baxter G. Role of reactive oxygen species on the formation of the novel diagnostic marker ischaemia modified albumin. Heart. 2006; 92, 113-114.
  • Sancaktutar AA, Bodakci MN, Hatipoglu NK, Soylemez H, Basarılı K, Turkcu G. The protective effects of pomegranate extracts against renal ischemia-reperfusion injury in male rats. Urology annals. 2014; 6, 46.
  • Slater TF. Free radical mechanisms in tissue injury, In: Cell Function and Disease. Springer, 1988;209-218.
  • Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clinical chemistry. 1988; 34, 497-500.
  • Tanrıverdi M, Karadağ F. Akut böbrek yetersizliği. Konuralp Tıp Dergisi. 2010; 2, 46-52.
  • Thiyagarajan M, Sharma SS. Neuroprotective effect of curcumin in middle cerebral artery occlusion induced focal cerebral ischemia in rats. Life sciences. 2004; 74, 969-985.
  • Uslu H, Yoruk O, Ayyildiz A, Aktan B. Antibacterial spectrum of Umckaloabo (Pelargonium sidoides) on upper airway infection agents. Eur. J. Gen. Med. 2009; 6, 245-248.
  • Yaqoob M, Edelstein CL, Wieder ED, Alkhunaizi AM, Gengaro PE, Nemenoff RA, Schrier RW. Nitric oxide kinetics during hypoxia in proximal tubules: effects of acidosis and glycine. Kidney international. 1996; 49, 1314-1319.
Birincil Dil en
Konular Fen
Dergi Bölümü ARAŞTIRMA MAKALESİ
Yazarlar

Yazar: Zülfükar SARITAŞ (Sorumlu Yazar)
Kurum: AKÜ Veteriner Fakültesi Cerrahi AD
Ülke: Turkey


Yazar: Hazen SARITAŞ
Kurum: SÜ Tıp Fak. Nefroloji BD. KONYA
Ülke: Turkey


Yazar: Musa KORKMAZ
Kurum: AKÜ Veteriner Fakültesi Cerrahi AD.
Ülke: Turkey


Yazar: M.Fatih BOZKURT
Kurum: AKÜ Veteriner Fakültesi Patoloji AD.
Ülke: Turkey


Yazar: Aziz BÜLBÜL
Kurum: AKÜ Veteriner Fakültesi Fizyoloji AD.
Ülke: Turkey


Yazar: Rengin ELSÜRER AFŞAR
Kurum: SDÜ Tıp Fak. Nefroloji BD. Isparta
Ülke: Turkey


Yazar: Tuba Berra SARITAŞ
Kurum: AKÜ Tıp Fak. Anesteziyoloji ve Reanimasyon AD. Afyonkarahisar
Ülke: Turkey


Bibtex @araştırma makalesi { kvj416649, journal = {Kocatepe Veteriner Dergisi}, issn = {1308-1594}, eissn = {2147-6853}, address = {Afyon Kocatepe Üniversitesi}, year = {2018}, volume = {11}, pages = {215 - 222}, doi = {10.30607/kvj.416649}, title = {Effects of Curcumin on A Renal Ischemia/Reperfusion Injury in Rats}, key = {cite}, author = {SARITAŞ, Zülfükar and SARITAŞ, Tuba Berra and ELSÜRER AFŞAR, Rengin and KORKMAZ, Musa and BOZKURT, M.Fatih and SARITAŞ, Hazen and BÜLBÜL, Aziz} }
APA SARITAŞ, Z , SARITAŞ, H , KORKMAZ, M , BOZKURT, M , BÜLBÜL, A , ELSÜRER AFŞAR, R , SARITAŞ, T . (2018). Effects of Curcumin on A Renal Ischemia/Reperfusion Injury in Rats. Kocatepe Veteriner Dergisi, 11 (3), 215-222. DOI: 10.30607/kvj.416649
MLA SARITAŞ, Z , SARITAŞ, H , KORKMAZ, M , BOZKURT, M , BÜLBÜL, A , ELSÜRER AFŞAR, R , SARITAŞ, T . "Effects of Curcumin on A Renal Ischemia/Reperfusion Injury in Rats". Kocatepe Veteriner Dergisi 11 (2018): 215-222 <http://dergipark.gov.tr/kvj/issue/37070/416649>
Chicago SARITAŞ, Z , SARITAŞ, H , KORKMAZ, M , BOZKURT, M , BÜLBÜL, A , ELSÜRER AFŞAR, R , SARITAŞ, T . "Effects of Curcumin on A Renal Ischemia/Reperfusion Injury in Rats". Kocatepe Veteriner Dergisi 11 (2018): 215-222
RIS TY - JOUR T1 - Effects of Curcumin on A Renal Ischemia/Reperfusion Injury in Rats AU - Zülfükar SARITAŞ , Hazen SARITAŞ , Musa KORKMAZ , M.Fatih BOZKURT , Aziz BÜLBÜL , Rengin ELSÜRER AFŞAR , Tuba Berra SARITAŞ Y1 - 2018 PY - 2018 N1 - doi: 10.30607/kvj.416649 DO - 10.30607/kvj.416649 T2 - Kocatepe Veteriner Dergisi JF - Journal JO - JOR SP - 215 EP - 222 VL - 11 IS - 3 SN - 1308-1594-2147-6853 M3 - doi: 10.30607/kvj.416649 UR - http://dx.doi.org/10.30607/kvj.416649 Y2 - 2018 ER -
EndNote %0 Kocatepe Veteriner Dergisi Effects of Curcumin on A Renal Ischemia/Reperfusion Injury in Rats %A Zülfükar SARITAŞ , Hazen SARITAŞ , Musa KORKMAZ , M.Fatih BOZKURT , Aziz BÜLBÜL , Rengin ELSÜRER AFŞAR , Tuba Berra SARITAŞ %T Effects of Curcumin on A Renal Ischemia/Reperfusion Injury in Rats %D 2018 %J Kocatepe Veteriner Dergisi %P 1308-1594-2147-6853 %V 11 %N 3 %R doi: 10.30607/kvj.416649 %U 10.30607/kvj.416649
ISNAD SARITAŞ, Zülfükar , SARITAŞ, Hazen , KORKMAZ, Musa , BOZKURT, M.Fatih , BÜLBÜL, Aziz , ELSÜRER AFŞAR, Rengin , SARITAŞ, Tuba Berra . "Ratlarda Renal İskemi/Reperfüzyon Hasarında Curcumin'in Etkileri". Kocatepe Veteriner Dergisi 11 / 3 (Eylül 2018): 215-222. http://dx.doi.org/10.30607/kvj.416649